Effect of a high fat diet on ovary morphology, in vitro development, in vitro fertilisation rate and oocyte quality in mice

Sohrabi, Maryam; Roushandeh, Amaneh Mohammadi; Alizadeh, Zohreh; Vahidinia, Aliasghar; Vahabian, Mehrangiz; Hosseini, Mehdi

doi:10.11622/smedj.2015085

Cited by 45 publications

(42 citation statements)

References 27 publications

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“…Evidence indicates that the detrimental effects of obesity on female reproduction likely originate from compromised oocyte quality (31)(32)(33)(34)(35). Specifically, meiotic defects and oxidative stress have been detected in oocytes from obese mice (7,36).…”

Section: Sirt7 Overexpression Ameliorates Maternal Obesity-induced Mementioning

confidence: 99%

SIRT7 functions in redox homeostasis and cytoskeletal organization during oocyte maturation

Gao

et al. 2018

FASEB j.

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SIRT7, a member of the sirtuin family, with coenzyme NAD catalyzes protein deacetylation and has been implicated in multiple biologic processes; however, its function in mammalian oocytes remains to be explored. Here, we report disrupted meiotic maturation upon specific knockdown of SIRT7 in mouse oocytes. In particular, disorganized spindle/chromosomes and the loss of the cortical actin cap are readily observed in SIRT7-depleted oocytes, generating aneuploid eggs. Furthermore, we found that SIRT7 depletion markedly elevated reactive oxygen species levels in oocytes, thereby compromising the developmental competence of early embryos. Of note, SIRT7 protein level is significantly decreased in oocytes from obese mice, and the forced expression of exogenous SIRT7 ameliorates maternal obesity-associated meiotic defects and oxidative stress in oocytes. In summary, our data suggest that SIRT7 is an essential factor in the determination of oocyte quality and may mediate the effects of obesity on female reproduction.-Gao, M., Li, X., He, Y., Han, L., Qiu, D., Ling, L., Liu, H., Liu, J., Gu, L. SIRT7 functions in redox homeostasis and cytoskeletal organization during oocyte maturation.

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Section: Sirt7 Overexpression Ameliorates Maternal Obesity-induced Mementioning

confidence: 99%

SIRT7 functions in redox homeostasis and cytoskeletal organization during oocyte maturation

Gao

et al. 2018

FASEB j.

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“…Obese women take longer to conceive, even if cycling regularly, and have a higher risk of miscarriage, preeclampsia, and congenital defects in the offspring . Evidence indicates that the detrimental effects of obesity on female reproduction likely originate from the compromised oocyte quality . In particular, increased pregnancy failure rate in obese women is reversed if donor oocytes are used instead of autologous oocytes through an in vitro fertilization (IVF) procedure …”

Section: Introductionmentioning

confidence: 99%

Melatonin protects against maternal obesity‐associated oxidative stress and meiotic defects in oocytes via the SIRT3‐SOD2‐dependent pathway

Han

Wang

et al. 2017

Journal of Pineal Research

145

124

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Maternal obesity in humans is associated with poor outcomes across the reproductive spectrum. Emerging evidence indicates that these defects are likely attributed to factors within the oocyte. Although various molecules and pathways may contribute to impaired oocyte quality, prevention of fertility issues associated with maternal obesity is a challenge. Using mice fed a high-fat diet (HFD) as an obesity model, we document spindle disorganization, chromosome misalignment, and elevated reactive oxygen species (ROS) levels in oocytes from obese mice. Oral administration of melatonin to HFD mice not only reduces ROS generation, but also prevents spindle/chromosome anomalies in oocytes, consequently promoting the developmental potential of early embryos. Consistent with this finding, we find that melatonin supplement during in vitro maturation also markedly attenuates oxidative stress and meiotic defects in HFD oocytes. Finally, by performing morpholino knockdown and acetylation-mimetic mutant overexpression assays, we reveal that melatonin ameliorates maternal obesity-induced defective phenotypes in oocytes through the SIRT3-SOD2-dependent mechanism. In sum, our data uncover the marked beneficial effects of melatonin on oocyte quality from obese females; this opens a new area for optimizing culture system as well as fertility management.

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“…In addition, obesity, has also been linked to dangerous effects on spermatogenesis, which can affect the fertilization rate. 3,4 Testosterone, the primary male sex hormone produced by Leydig cells, it plays a significant role in the development of male reproductive tissues and spermatogenesis.…”

Section: Introductionmentioning

confidence: 99%

Protective Role of Eurycoma longifolia Jack Root Extract Against High-Fat Diet Induced Testicular Damage in Sprague-Dawley Rats

Al-Ani¹,

Ku-Zaifah²,

Al-Joufi³

et al. 2019

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Objective: The aims of this study were to investigate the adverse effects of the high-fat-diet (HFD) on the testosterone level and testicular structure of male rats as well as to examine whether Eurycoma Longifolia (EL) is able to ameliorate these effects. Methods: Twenty-four male Sprague-Dawley (SD) rats were divided into four groups each containing 6 rats. Group ND was given only normal diet, group NDEL was given normal diet and EL extracts (15 mg/ kg) dissolved in distilled water, group HFD was given only high-fat-diet and group HFDEL was given high-fat-diet and EL extracts (15 mg/kg). EL was administered orally for 12 weeks. The animal's testosterone level was measured at week 0, 6 and 12. The rats were sacrificed at the end of 12 th weeks and the testes samples were processed for histological examination Results: The testosterone level was significantly increased (p < 0.05) in the in the treated rats with EL (NDEL and HFDEL) compared with ND and HFD groups. Treatment with HFD revealed a marked degeneration of the seminiferous tubule epithelium and disruption of interstitial cells of the testis thereby interfering with spermatogenesis. Treatment of HFD rats with El reduced the adverse effects of HFD and improved the morphological structure of the seminiferous tubules. Conclusion: These findings revealed that EL has ameliorative effects against the testicular damage caused by high-fat-diet.

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Effect of a high fat diet on ovary morphology, in vitro development, in vitro fertilisation rate and oocyte quality in mice

Abstract: An HFD reduces folliculogenesis in the primordial and Graafian stages, in vitro maturation and in vitro fertilisation rates, as well as oocyte quality in mice.

Cited by 45 publications

References 27 publications

SIRT7 functions in redox homeostasis and cytoskeletal organization during oocyte maturation

SIRT7 functions in redox homeostasis and cytoskeletal organization during oocyte maturation

Melatonin protects against maternal obesity‐associated oxidative stress and meiotic defects in oocytes via the SIRT3‐SOD2‐dependent pathway

Protective Role of Eurycoma longifolia Jack Root Extract Against High-Fat Diet Induced Testicular Damage in Sprague-Dawley Rats

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