Effect of a growth hormone infusion on plasma insulin-like growth factor-I in Meishan and Large White pigs

Louveau, Isabelle; Bonneau, Michel

doi:10.1051/rnd:19960307

Cited by 49 publications

(31 citation statements)

References 18 publications

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“…The plasma concentration of haptoglobin was measured using a colorimetric method and haptoglobin assay kit based on binding of haptoglobin to hemoglobin (Tridelta Ltd, Maynooth, Co. Kildare, Ireland). Plasma IGF-I concentration was measured using a double-antibody RIA (Louveau and Bonneau, 1996) after an acid-ethanol extraction. CBG, haptoglobin and IGF-I concentrations were measured in basal blood samples only.…”

Section: Animalsmentioning

confidence: 99%

The cortisol response to ACTH in pigs, heritability and influence of corticosteroid-binding globulin

Larzul

Terenina

Foury

et al. 2015

Animal

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In the search for biological basis of robustness, this study aimed (i) at the determination of the heritability of the cortisol response to ACTH in juvenile pigs, using restricted maximum likelihood methodology applied to a multiple trait animal model, and (ii) at the study of the relationships between basal and stimulated cortisol levels with corticosteroid-binding globulin (CBG), IGF-I and haptoglobin, all important players in glucose metabolism and production traits. At 6 weeks of age, 298 intact male and female piglets from 30 litters (30 dams and 30 boars) were injected with 250 µg ACTH(1-24) (Synacthen). Blood was taken before ACTH injection to measure basal levels of cortisol, glucose, CBG, IGF-I and haptoglobin, and 60 min later to measure stimulated cortisol levels and glucose. Cortisol increased 2.8-fold after ACTH injection, with a high correlation between basal and stimulated levels (phenotypic correlation, r p = 0.539; genetic correlation, r g = 0.938). Post-ACTH cortisol levels were highly heritable ( h 2 = 0.684) and could therefore be used for genetic selection of animals with a more reactive hypothalamic-pituitary-adrenocortical axis. CBG binding capacity correlated with cortisol levels measured in basal conditions in males only. No correlation was found between CBG binding capacity and post-ACTH cortisol levels. Basal IGF-I concentration was positively correlated with BW at birth and weaning, and showed a high correlation with CBG binding capacity with a strong sexual dimorphism, the correlation being much higher in males than in females. Basal haptoglobin concentrations were negatively correlated with CBG binding capacity and IGF-I concentrations. Complex relationships were also found between circulating glucose levels and these different variables that have been shown to be related to glucose resistance in humans. These data are therefore valuable for the genetic selection of animals to explore the consequences on production and robustness traits, but also point at pigs as a relevant model to explore the underlying mechanisms of the metabolic syndrome including the contribution of genetic factors.

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Section: Animalsmentioning

confidence: 99%

The cortisol response to ACTH in pigs, heritability and influence of corticosteroid-binding globulin

Larzul

Terenina

Foury

et al. 2015

Animal

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“…Plasma IGF-1 concentrations were quantified in duplicate, using a commercial kit (IRMA IGF-1 A15729 R ; Immunotech, Marseille, France), after extraction of the samples with ethanol/HCl (as validated by Louveau and Bonneau, 1996). Sensitivity was 2 ng/ml, intra-assay CV was 2.2% (n 5 26) and inter-assay CV was 3.5% (n 5 12).…”

Section: General Designmentioning

confidence: 99%

Insulin-stimulating diets during the weaning-to-estrus interval do not improve fetal and placental development and uniformity in high-prolific multiparous sows

Wientjes

Soede

Laurenssen

et al. 2013

Animal

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Piglet birth weight and litter uniformity are important for piglet survival. Insulin-stimulating sow diets before mating may improve subsequent piglet birth weights and litter uniformity, but the physiological mechanisms involved are not clear. This study evaluated effects of different levels of insulin-stimulating feed components (dextrose plus starch; fed twice daily) during the weaningto-estrus interval (WEI) on plasma insulin and IGF-1 concentrations, and on follicle development and subsequent luteal, fetal and placental development and uniformity at days 42 to 43 of pregnancy. During WEI, multiparous sows were isocalorically fed diets supplemented with 375 g/day dextrose plus 375 g/day corn starch (INS-H), with 172 g/day dextrose plus 172 g/day corn starch and 144 g/day animal fat (INS-L), or with 263 g/day animal fat (CON). Jugular vein catheters were inserted through the ear vein at 1.5 days before weaning to asses plasma insulin and IGF-1 concentrations. After estrus, all sows received a standard gestation diet until slaughter at days 42 to 43 of pregnancy. The dextrose plus starch-diets enhanced the postprandial insulin response in a dose-dependent manner (e.g. at day 2 insulin area under the curve was 4516 mU/444 min for CON, 8197 mU/444 min for INS-L and 10 894 mU/444 min for INS-H; s.e.m. 5 694; P , 0.001), but did not affect plasma IGF-1 concentrations during the first 3 days of WEI. Follicle development and subsequent luteal, fetal and placental development and uniformity were not affected by the dietary treatments, nor related to plasma insulin and IGF-1 concentrations during WEI. Pre-weaning plasma insulin and IGF-1 concentrations were negatively related to sow body condition loss during lactation, but were not related to subsequent reproduction characteristics. This study shows that dietary dextrose plus starch are effective in stimulating insulin secretion (both postprandial peak and long-term concentration), but not IGF-1 secretion during the first 3 days after weaning in multiparous sows. The extreme insulin-stimulating diets during WEI did, however, not improve follicle development, or subsequent development and uniformity of fetuses and placentas in these high-prolific sows (27.0 6 0.6 ovulations; 18.6 6 0.6 vital fetuses).

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“…Plasma insulin and IGF-I concentrations were determined in duplicates using validated RIA [15,16]. The samples were run in a single assay.…”

Section: Hormone Assaysmentioning

confidence: 99%

“…Plasma IGF-I concentrations were determined after an acid-ethanol extraction according to Louveau and Bonneau [16]. The intra-assay CV was 11% at 48.7 ng·mL -1 , and the average sensitivity of the assay, defined as 90% of total binding, was 0.31 ng·mL -1 .…”

Section: Hormone Assaysmentioning

confidence: 99%

Effects of a bovine colostrum-supplemented diet on some gut parameters in weaned piglets

et al. 2006

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-The present study investigated the effects of a bovine colostrum-supplemented diet on gut post-weaning adaptation and health in piglets. Thirty-six 21-d-old piglets were allocated to one of the three following dietary treatments: sow-reared (SR), weaned on a control starter diet (WCtrl) or on a starter diet supplemented with bovine colostrum (WCol) until slaughter at 28 d or 35 d of age. Gastric pH and intestinal bacteriological, structural and functional parameters were determined. Compared to WCtrl, the gastric pH was lower (P < 0.05) and the duodenal lactobacilli:coliform ratio was higher (P = 0.05) in WCol piglets. The relative small intestine weight was 18% (P < 0.05) higher in WCol piglets than in SR piglets. Duodenal villous height was lower (P < 0.01) in WCtrl than in SR piglets, whereas the value for WCol piglets was intermediate. The weaning-increased crypt cell proliferation was not affected by bovine colostrum supplementation. The mucosal ribosomal capacity was higher (P < 0.05) in W than in SR piglets. In conclusion, a diet supplemented with colostrum induced, although not always significantly, variations of gut parameters, suggesting that globally, colostrum may limit weaning-induced gut structural and microbial alterations. The observed effects occurred early and were maintained throughout the post-weaning adaptive phase. bovine colostrum / intestine / microflora / mucosa growth / weaning

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Effect of a growth hormone infusion on plasma insulin-like growth factor-I in Meishan and Large White pigs

Cited by 49 publications

References 18 publications

The cortisol response to ACTH in pigs, heritability and influence of corticosteroid-binding globulin

The cortisol response to ACTH in pigs, heritability and influence of corticosteroid-binding globulin

Insulin-stimulating diets during the weaning-to-estrus interval do not improve fetal and placental development and uniformity in high-prolific multiparous sows

Effects of a bovine colostrum-supplemented diet on some gut parameters in weaned piglets

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