Effect of a fibrin(ogen)-derived vasoactive peptide on polymorphonuclear leukocyte emigration

Saldeen, K.; Christie, Natasha; Nelson, Warrick; Mova, H.Z.

doi:10.1016/0049-3848(85)90035-0

Cited by 14 publications

(6 citation statements)

References 8 publications

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“…Thus, it is expected that inflammatory cells would be recruited to sites of clot formation by chemotactic agents produced by the clot itself. Indeed, fibrin‐derived peptides have been shown to act as chemotactic agents in vitro 30–32 and in vivo ,31 and neutrophils have been shown to have specific receptors for clot constituents 33, 34. Fibrin peptides have also been shown to stimulate other leukocyte functions, such as degranulation 15.…”

Section: Discussionmentioning

confidence: 99%

Optimal Packing Characteristics of Rolled, Continuous Stationary‐Phase Columns

Ladisch

Yang

et al. 2002

Biotechnology Progress

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Rolled, continuous stationary phases were constructed by tightly rolling and inserting a whole textile fabric into a chromatography column. This work reports the column performance, in terms of plate height, void fraction, and resolution, of 10 cellulose-based fabrics. The relation between fabric structural properties of yarn diameter, fabric count, fabric compressibility, and column performance are quantitated. General requirements, including reproducibility of packing, for choosing fabrics to make a good SEC column are identified. This research showed that the packed columns have an optimal mass of fabric that minimizes plate height and maximizes resolution, in a manner that is consistent with chromatography theory. Mass of material packed is then an important column parameter to consider when optimizing columns for the rapid desalting of proteins. Proteins were completely separated from salt and glucose in less than 8 min at a pressure drop less than 500 psi on the rolled, continuous stationary-phase columns. These results, together with stability and reproducibility, suggest potential industrial applications for cellulose-based rolled, continuous stationary-phase columns where speed is a key parameter in the production process.

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Section: Discussionmentioning

confidence: 99%

Optimal Packing Characteristics of Rolled, Continuous Stationary‐Phase Columns

Ladisch

Yang

et al. 2002

Biotechnology Progress

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“…However, the apparent proinflammatory effects of fibrin(ogen) may involve indirect interactions. For example, some fibrin degradation products (81)(82)(83)(84)(85) and fibrinopeptide B (86) are strongly chemotactic for PMN, monocytes and fibroblasts. In addition, lower molecular weight degradation products of fibrin(ogen) are known to promote granulocyte infiltration and vascular permeability (82,87).…”

Section: Discussionmentioning

confidence: 99%

Fibrin(ogen) mediates acute inflammatory responses to biomaterials.

Tang

Eaton

1993

The Journal of Experimental Medicine

423

360

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SummaryAlthough "biocompatible" polymeric elastomers are generally nontoxic, nonimmunogenic, and chemically inert, implants made of these materials may trigger acute and chronic inflammatory responses. Early interactions between implants and inflammatory cells are probably mediated by a layer of host proteins on the material surface. To evaluate the importance of this protein layer, we studied acute inflammatory responses of mice to samples of polyester terephthalate film (PET) that were implanted intraperitoneally for short periods. Material preincubated with albumin is "passivated;' accumulating very few adherent neutrophils or macrophages, whereas uncoated or plasma-coated PET attracts large numbers ofphagocytes. Neither IgG adsorption nor surface complement activation is necessary for this acute inflammation; phagocyte accumulation on uncoated implants is normal in hypogammaglobulinemic mice and in severely hypocomplementemic mice. Rather, spontaneous adsorption of fibrinogen appears to be critical : (a) PET coated with serum or hypofibrinogenemic plasma attracts as few phagocytes as does albumin-coated material; (b) in contrast, PET preincubated with serum or hypofibrinogenemic plasma containing physiologic amounts of fibrinogen elicits "normal" phagocyte recruitment; (c) most importantly, hypofibrinogenemic mice do not mount an inflammatory response to implanted PET unless the material is coated with fibrinogen or the animals are injected with fibrinogen before implantation. Thus, spontaneous adsorption of fibrinogen appears to initiate the acute inflammatory response to an implanted polymer, suggesting an interesting nexus between two major iatrogenic effects of biomaterials : clotting and inflammation .

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“…FDPs reportedly stimulate vascular permeability, 82 endothelial cell retraction, 83,84 monocyte/macrophage IL-1 and IL-6 production, 85,86 and leukocyte chemotaxis. [87][88][89] FDPs can be generated via plasmin-mediated proteolysis of fibrin, and mice with reduced or no plasmin have been generated by gene-targeted deletion of plasminogen activators or plasminogen, respectively. 90,91 Such plasmin-deficient mice display increased pathology in glomerulonephritis 92 and arthritis 51,93 models, suggesting that plasmingenerated FDPs may well function in inflammation.…”

Section: Discussionmentioning

confidence: 99%

Roles for thrombin and fibrin(ogen) in cytokine/chemokine production and macrophage adhesion in vivo

Szaba

Smiley

2002

Blood

286

224

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Extravascular coagulation leading to fibrin deposition accompanies many immune and inflammatory responses. Although recognized by pathologists for decades, and probably pathologic under certain conditions, the physiologic functions of extravascular coagulation remain to be fully defined. This study demonstrates that thrombin can activate macrophage adhesion and prompt interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) production in vivo. Peritoneal macrophages were elicited with thioglycollate (TG) and then activated in situ, either by intraperitoneal injection of lipopolysaccharide (LPS) or by injection of antigen into mice bearing antigen-primed T cells. Others previously established that such treatments stimulate macrophage adhesion to the mesothelial lining of the peritoneal cavity. The present study demonstrates that thrombin functions in this process, as macrophage adhesion was suppressed by Refludan, a highly specific thrombin antagonist, and induced by direct peritoneal administration of purified thrombin. Although recent studies established that protease activated receptor 1 (PAR-1) mediates some of thrombin's proinflammatory activities macrophage adhesion occurred normally in PAR-1-deficient mice. However, adhesion was suppressed in fibrin(ogen)-deficient mice, suggesting that fibrin formation stimulates macrophage adhesion in vivo. This study also suggests that fibrin regulates chemokine/cytokine production in vivo, as direct injection of thrombin stimulated peritoneal accumulation of IL-6 and MCP-1 in a fibrin(ogen)-dependent manner. Given that prior studies have clearly established inflammatory roles for PAR-1, thrombin probably has pleiotropic functions during inflammation, stimulating vasodilation and mast cell degranulation via PAR-1, and activating cytokine/chemokine production and macrophage adhesion via fibrin(ogen). IntroductionVasodilation and increased vascular permeability are among the earliest signs of inflammation. These events stimulate the extravasation of inactive coagulant precursors, which become activated upon exposure to extravascular tissues. The ensuing coagulation cascade culminates with the generation of thrombin, a protease that cleaves extravasated fibrinogen, prompting its polymerization and deposition as fibrin. Accordingly, localized extravascular fibrin deposition accompanies many type 1 T helper cell (Th1)-associated responses, including autoimmune neuropathologies, 1-4 glomerulonephritis, 5,6 rheumatoid arthritis, 7-9 Crohn's disease, 10,11 allograft rejection, 12,13 delayed-type hypersensitivity, 14-19 and viral infections. 20,21 For some time, it has been appreciated that such Th1-associated coagulation has physiologic consequences, as the swelling that accompanies delayed-type hypersensitivity responses is suppressed in anticoagulated or fibrinogen-deficient subjects. [14][15][16][17][18][19] However, the full significance of immune-associated extravascular coagulation remains to be defined.Recent studies suggest that thrombin is a physiologic m...

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Effect of a fibrin(ogen)-derived vasoactive peptide on polymorphonuclear leukocyte emigration

Cited by 14 publications

References 8 publications

Optimal Packing Characteristics of Rolled, Continuous Stationary‐Phase Columns

Optimal Packing Characteristics of Rolled, Continuous Stationary‐Phase Columns

Fibrin(ogen) mediates acute inflammatory responses to biomaterials.

Roles for thrombin and fibrin(ogen) in cytokine/chemokine production and macrophage adhesion in vivo

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