Effect of A-factor on the growth of asporogenous mutants ofStreptomyces griseus, not producing this factor

Khokhlov, A. S.; Anisova, L. N.; Tovarova, I I; Kleiner, E. M.; Коваленко, И. В.; Krasilnikova, O. I.; Kornitskaya, E. Ya.; Pliner, S. A.

doi:10.1002/jobm.3630130803

Cited by 78 publications

(12 citation statements)

References 10 publications

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“…Members of class II sporulate only when grown in juxtaposition to the wild-type strain or bald mutants of class III or IV. Superficially, the strains in class II resemble afsA mutants, described by Hara and Beppu (7) and Khokhlov et al (17) as bald, A-factornonproducing isolates of S. griseus that could be cross-fed to sporulate by strains capable of producing A-factor. Unlike the streptomycin-sensitive afsA mutants described by Hara and Beppu, however, our class II mutants appear to haveretained the parental level of resistance to streptomycin (unpublished observation).…”

Section: Discussionmentioning

confidence: 86%

“…(i) The phenotype of our class III mutants, from which the initial cloning recipient was selected, is unlike the phenotype of A-factor nonproducers (7,17). Whereas the latter can be cross-fed to sporulate by the wild-type strain and other A-factor-producing isolates, our class III bald mutants are unresponsive to the wild-type strain.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have suggested a regulatory link between the production of secondary metabolites, such as antibiotics, and the ability to sporulate (3,6,7,11,17,25). Some strains of Streptomyces griseus that fail to produce the antibiotic streptomycin simultaneously lose the ability to sporulate and to produce the low-molecular-weight positive regulator of secondary metabolism, A-factor (7,17).…”

Section: Piret Personal Communication)mentioning

confidence: 99%

“…Some strains of Streptomyces griseus that fail to produce the antibiotic streptomycin simultaneously lose the ability to sporulate and to produce the low-molecular-weight positive regulator of secondary metabolism, A-factor (7,17). A fragment of DNA that restores A-factor production, antibiotic synthesis, and sporulation to A-factor-nonproducing mutants of S. bikiniensis (afsA) has been cloned and characterized (11,12).…”

Section: Piret Personal Communication)mentioning

confidence: 99%

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Cloning of DNA involved in sporulation of Streptomyces griseus

Babcock

Kendrick

1988

J Bacteriol

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Twenty-two bald mutants of Streptomyces griseus were isolated and classified into four phenotypic groups, two of which showed conditional sporulation. A 3-kilobase fragment of DNA was cloned in a high-copy-number vector and detected by its ability to restore sporulation to one class of conditionally bald mutants. Analysis of subclones demonstrated that the sporulation property was contained within a 2.5-kilobase fragment. Hybridization studies and restriction analysis indicated that this DNA fragment was present in several Streptomyces species and was distinct from DNA that has been shown to complement afsA mutants of S. bikiniensis and bldA mutants of S. coelicolor.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Piret Personal Communication)mentioning

confidence: 99%

Section: Piret Personal Communication)mentioning

confidence: 99%

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Cloning of DNA involved in sporulation of Streptomyces griseus

Babcock

Kendrick

1988

J Bacteriol

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“…A-factor, 2-S-isocapryloyl-3-S-hydroxymethyl-y-butyrolactone, found in Streptomyces griseus and Streptomyces bikiniensis (Khokhlov et al, 1973), is a potent autoregulating factor essential for both streptomycin biosynthesis and spore formation in these organisms (Khokhlov et al, 1973;Khokhlov, 1980; Hara & Beppu, 1982a). Furthermore, recent work from this laboratory has revealed that A-factor is also involved in streptomycin resistance in these organisms through its inducible effect on the synthesis of streptomycin-6-phosphotransferase (Hara & Beppu, 1982 b).…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis of A-factor Synthesis in Streptomyces coelicolor A3(2) and Streptomyces griseus

et al. 1983

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A-factor is a potent pleiotropic effector produced by Streptomyces griseus and is essential for streptomycin production and spore formation in this organism. Its production is widely distributed among various actinomycetes including Streptomyces coelicolor A3(2). Genetic analysis of A-factor production was carried out with S . coelicolor A3(2), and two closely linked loci for A-factor mutations (afsA and B) were identified between cysD and leuB on the chromosomal linkage map. In contrast, genetic crosses of A-factor-negative mutants of S. griseus, using a protoplast fusion technique, failed to give a fixed locus for A-factor gene(s) and suggested involvement of an extrachromosomal or transposable genetic element in A-factor synthesis in this organism. INTRODUCTIONA-factor, 2-S-isocapryloyl-3-S-hydroxymethyl-y-butyrolactone, found in Streptomyces griseus and Streptomyces bikiniensis (Khokhlov et al., 1973), is a potent autoregulating factor essential for both streptomycin biosynthesis and spore formation in these organisms (Khokhlov et al., 1973;Khokhlov, 1980; Hara & Beppu, 1982a). Furthermore, recent work from this laboratory has revealed that A-factor is also involved in streptomycin resistance in these organisms through its inducible effect on the synthesis of streptomycin-6-phosphotransferase (Hara & Beppu, 1982 b). Mutants lacking A-factor simultaneously lose streptomycin production capability, the ability to form spores and a large degree of streptomycin resistance. Exogenous supplementation of A-factor to the culture of these mutants restores all these phenotypes. A-factor-negative mutants of S . griseus and S. bikiniensis were easily obtained at high frequency by treatment with acridine dyes or by incubation at high temperature, which suggested involvement of an extrachromosomal genetic determinant in the biosynthesis of A-factor in these organisms.A-factor productivity has been found in various species of actinomycetes including Streptomyces coelicolor A3(2) (Efremenkova et al., 1979; Hara & Beppu, 1982a), but in contrast to S . griseus and S. bikiniensis, no A-factor-negative mutants of S. coelicolor A3(2) were obtained by the curing treatments. In this report, we describe genetic analyses of A-factor genes of S . coelicolor A3(2) and S . griseus, using conjugation and protoplast fusion techniques, respectively. The results clearly indicate that A-factor gene(s) of S. coelicofor A3(2) are located at a fixed position on the chromosomal linkage map, while in S . griseus, they appear to be extrachromosomal.t Present address: Fermentation Technology Laboratories, Meiji

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