Effect of a derivatized dextran on human osteoblast growth and phenotype expression

Berrada, Saadia; Amédée, Joëlle; Avramoglou, Thierry; Jozefonvicz, J.; Harmand, Marie‐Françoise

doi:10.1163/156856294x00329

Cited by 16 publications

(11 citation statements)

References 30 publications

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“…4C) and Runx2 (Fig. 4D) mRNA levels compared to control cells (S) ( P < 0.05), which supports previous findings with synthetic, HS‐like molecules (Berrada et al, 1994). However, these increases were abolished when HS was added in combination with FGF‐2.…”

Section: Resultssupporting

confidence: 89%

“…HS‐like molecules have previously been shown to increase and decrease cell proliferation in human osteoblasts (Berrada et al, 1994) and in MC3T3‐E1 cells, respectively (Blanquaert et al, 1999). This distinction between the different HS species in regulating proliferation may arise due to HS specificities present on the different proteoglycans.…”

Section: Discussionmentioning

confidence: 98%

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Heparan sulfate regulates the anabolic activity of MC3T3‐E1 preosteoblast cells by induction of Runx2

Jackson

Murali

Wijnen

et al. 2006

Journal Cellular Physiology

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The transcription factor Runx2 can be controlled by a number of upstream regulators involved in intracellular signaling, including the activation ERK1/2 signaling by fibroblast growth factor-2 (FGF-2). FGFs interact with their cell surface receptors (FGFRs) through an obligate cross-binding interaction with heparan sulfate proteoglycan (HSPG) co-receptors; exogenous HS sugar chains have been shown to potently modulate changes in cell phenotype depending on the stage of tissue differentiation when the HS is harvested, suggesting that HS chain structure and function varies depending on the stage of cell maturity. This study examined the potential of bone-derived heparan sulfate (HS), harvested from differentiating osteoblasts, for the enhancement of preosteoblast growth and differentiation. HS was harvested from conditioned media, cell surface and matrix compartments of postconfluent (differentiating) MC3T3-E1 osteoblasts and dosed back onto preconfluent MC3T3-E1 cells. We show that HS can increase the expression Runx2, ALP, and OPN in preosteoblast cells, suggesting the potential for exogenous HS to shift cells from proliferative to differentiative phenotypes. In line with their structural differences, only HS released into the media was found to co-stimulate the mitogenic effect of FGF-2, whilst exogenous application of all the HSs together with FGF-2 served to increase the expression of OPN. Only the application of cell surface-derived HS triggered a synergistic increase in FGFR1 expression together with FGF-2, although all three HS preparations could trigger transient increases in PI3K, ERK1/2, and stat3 phosphorylation levels. These findings demonstrate that the compartmentally distinct HS species expressed by differentiating MC3T3-E1 cells act in complex ways to coordinate the extracellular conditions that lead to osteoblast differentiation, with the cell surface species coordinating the FGF response. J. Cell. Physiol. 210: 38-50, 2007. ß 2006 The orderly progression of bone stem cells through their precursor stages into mature phenotypes requires a systematic cascade of tissue-specific transcription factors to coordinate the process. Of these transcription factors, Runx2, a member of the runt-related family of transcription factors, has been shown to be essential for both skeletal patterning during embryogenesis and the progression of osteoblast differentiation (Ducy et al

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Section: Resultssupporting

confidence: 89%

Section: Discussionmentioning

confidence: 98%

Heparan sulfate regulates the anabolic activity of MC3T3‐E1 preosteoblast cells by induction of Runx2

Jackson

Murali

Wijnen

et al. 2006

Journal Cellular Physiology

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“…Dextran derivatives substituted with carboxymethyl benzylamide sulfonate (CMDBS) have been shown to mimic heparin/HS by providing a somewhat similar protection and stabilization capability for growth factors as imparted by HS; albeit with less affinity. Otherwise known as 'regenerating agents' (RGTAs), these HS‐like molecules have been shown to stimulate tissue repair in skin, bone, muscle and the cornea [38–44]. In all cases, these HSlike molecules are thought to exert their effect by increasing both cell proliferation and differentiation within the wound site, presumably by enhancing growth factor‐mediated signals.…”

Section: Accelerated Bone Repair With Growth Factors Hs and Hs‐like mentioning

confidence: 99%

Concepts of scaffold‐based tissue engineering—the rationale to use solid free‐form fabrication techniques

Hutmacher

Cool

2007

J Cellular Molecular Medi

229

174

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A paradigm shift is taking place in orthopaedic and reconstructive surgery from using medical devices and tissue grafts to a tissue engineering approach that uses biodegradable scaffolds combined with cells or biological molecules to repair and/or regenerate tissues. One of the potential benefits offered by solid free-form fabrication technology (SFF) is the ability to create scaffolds with highly reproducible architecture and compositional variation across the entire scaffold, due to its tightly controlled computer-driven fabrication. In this review, we define scaffold properties and attempt to provide some broad criteria and constraints for scaffold design in bone engineering.We also discuss the application-specific modifications driven by surgeon's requirements in vitro and/or in vivo. Next, we review the current use of SFF techniques in scaffold fabrication in the context of their clinical use in bone regeneration. Lastly, we comment on future developments in our groups, such as the functionalization of novel composite scaffolds with combinations of growth factors; and more specifically the promising area of heparan sulphate polysaccaride immobilization within the bone tissue engineering arena.

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“…Previous tissue culture studies have shown that E9, another form of derivatized dextran, can increase osteoblast proliferation in a dose-responsive manner, inhibiting proliferation at high doses, and can also increase the expression of ALP, collagen I, OC and ON (Berrada et al, 1994). Therefore, it is assumed that HS-like molecules exert their effect by increasing both cell proliferation and differentiation within the wound site, presumably by enhancing growth factor-mediated signals.…”

Section: Accelerated Fracture Repair With Hs and Hs-like Moleculesmentioning

confidence: 99%

Coordinated fibroblast growth factor and heparan sulfate regulation of osteogenesis

Jackson

Nurcombe

Cool

2006

Gene

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Effect of a derivatized dextran on human osteoblast growth and phenotype expression

Cited by 16 publications

References 30 publications

Heparan sulfate regulates the anabolic activity of MC3T3‐E1 preosteoblast cells by induction of Runx2

Heparan sulfate regulates the anabolic activity of MC3T3‐E1 preosteoblast cells by induction of Runx2

Concepts of scaffold‐based tissue engineering—the rationale to use solid free‐form fabrication techniques

Coordinated fibroblast growth factor and heparan sulfate regulation of osteogenesis

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