Effect of a Defined Continuous-Flow Derived Bacterial Culture and Dietary Lactose on Salmonella typhimurium Colonization in Broiler Chickens

“…The cecal acidification observed in these experiments was also reported by other investigators and is to the production of organic acids resulting from carbohydrate fermentation by the intestinal microflora of chickens (8,9,22,23,28,32). ACM alone or in combination with lactose reduced cecal pH during treatment after challenge with S. Typhimurium and S. Enteritidis.…”

Section: Discussionsupporting

confidence: 85%

“…in the intestine of chickens (2,4,8,9,18,19,22,23,28,32). Its effect is due to the fact that lactose is not fully digested by chickens because of their lactase deficiency, with the carbohydrate reaching the cecum in practically intact form, and being fermented there by the cecal microflora which produces antagonistic substances against Salmonella spp.…”

Section: Resultsmentioning

confidence: 99%

Use of anaerobic cecal microflora, lactose and acetic acid for the protection of broiler chicks against experimental infection with Salmonella Typhimurium and Salmonella enteritidis

Filho¹,

Silva²,

Ribeiro³

et al. 2000

Braz. J. Microbiol.

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The effects of treatment with anaerobic cecal microflora (ACM) and/or lactose and/or acetic acid on systemic and digestive tract of broiler chicks infection with Salmonella Typhimurium and S. Enteritidis were studied. ACM was used without previous bacterial identification. Treatment with ACM contributed to the resistance of broiler chicks to infection with Salmonella spp. The infections were more persistent in the cecum, rectum and crops in decreasing order of intensity. The infections were also self-limiting since treated and control lots presented similar infection rates at the end of the experiments. Alone or in combination with lactose, ACM reduced the colonization of the digestive tract of broiler chicks by S. Typhimurium and S. Enteritidis. The effect of the combination of ACM with lactose or acetic acid was not potentiated in terms of reduction of fecal excretion of Salmonella spp. Treatment with ACM reduced the amount of S. Typhimurium and S. Enteritidis in the feces. Alone or in combination with lactose, ACM reduced the cecal pH in treated birds. S. Enteritidis was much more invasive than S. Typhimurium and the use of ACM alone was more effective on the reduction of systemic infection. An explanation for the process of prevention of intestinal colonization with Salmonella spp. probably resides in the interrelationship of physiological, microbiological and immunological phenomena, as well as the variation in cecal pH.

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“…The earlier culture-based studies generally isolated cecal bacteria from the contents of the ceca, and several of the competitive exclusion preparations studied over the years were also based on bacteria from the content of the gut (7,29,31). One preparation (45,46), however, is based on scrapings of the mucosal wall of the cecum.…”

Section: Discussionmentioning

confidence: 99%

16S rRNA-Based Analysis of Microbiota from the Cecum of Broiler Chickens

Zhu

Zhong

Pandya

et al. 2002

Appl Environ Microbiol

397

324

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The microbiota of the intestinal tract of chickens plays an important role in inhibiting the establishment of intestinal pathogens. Earlier culturing and microscopic examinations indicated that only a fraction of the bacteria in the cecum of chickens could be grown in the laboratory. Therefore, a survey of cecal bacteria was done by retrieval of 16S rRNA gene sequences from DNA isolated from the cecal content and the cecal mucosa. The ribosomal gene sequences were amplified with universal primers and cloned or subjected to temporal temperature gradient gel electrophoresis (TTGE). Partial 16S rRNA gene sequences were determined from the clones and from the major bands in TTGE gels. A total of 1,656 partial 16S rRNA gene sequences were obtained and compared to sequences in the GenBank. The comparison indicated that 243 different sequences were present in the samples. Overall, sequences representing 50 phylogenetic groups or subgroups of bacteria were found, but approximately 89% of the sequences represented just four phylogenetic groups (Clostridium leptum, Sporomusa sp., Clostridium coccoides, and enterics). Sequences of members of the Bacteroides group, the Bifidobacterium infantis subgroup, and of Pseudomonas sp. each accounted for less than 2% of the total. Sequences related to those from the Escherichia sp. subgroup and from Lactobacillus, Pseudomonas, and Bifidobacterium spp. were generally between 98 and 100% identical to sequences already deposited in the GenBank. Sequences most closely related to those of the other bacteria were generally 97% or less identical to those in the databases and therefore might be from currently unknown species. TTGE and random cloning indicated that certain phylogenetic subgroups were common to all birds analyzed, but sequence data from random cloning also provided evidence for qualitative and quantitative differences among the cecal microbiota of individual birds reared under very similar conditions.

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Effect of a Defined Continuous-Flow Derived Bacterial Culture and Dietary Lactose on Salmonella typhimurium Colonization in Broiler Chickens

Cited by 70 publications

References 17 publications

Isolation and Characterization of a New T-Even Bacteriophage, CEV1, and Determination of Its Potential To Reduce Escherichia coli O157:H7 Levels in Sheep

Isolation and Characterization of a New T-Even Bacteriophage, CEV1, and Determination of Its Potential To Reduce Escherichia coli O157:H7 Levels in Sheep

Use of anaerobic cecal microflora, lactose and acetic acid for the protection of broiler chicks against experimental infection with Salmonella Typhimurium and Salmonella enteritidis

16S rRNA-Based Analysis of Microbiota from the Cecum of Broiler Chickens

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