Effect of a Combination of Water Clover (Marsilea Crenata) Ethanol Extract and Moringa Leaf (Moringa oleifera) Ethanol Extract on Follicle and Oocyte Diameter of Goat: In Vitro Study

Abstract

Water clover (Marsilea crenata) and moringa leaves (Moringa oleifera) are types of herbal plants that are widely used in various alternative medicines. Pharmacologically, both have active compounds such as flavonoids which have cellular activities such as antioxidants and estrogenic. This study aims to evaluate the effect of a combination of water clover ethanol extract and Moringa leaf ethanol extract with different treatment groups with the same concentration on follicle and oocyte growth based on follicle diameter and oocyte diameter after six days of culture. This experimental study used 24 antral follicles (2.5-3.2 mm) which were isolated, and randomly divided into four treatment groups namely K (control) without supplementation of water clover ethanol extract and moringa leaf ethanol extract, group D1 with water clover ethanol extract, group D2 with supplementation of moringa leaf ethanol extract, and group D3 with supplementation of a combination of water clover ethanol extract and moringa leaf