Effect of 9-β-
            <scp>d</scp>
            -Arabinofuranosyladenine on Polymer Synthesis in a Polyauxotrophic Strain of
            <i>Escherichia coli</i>

Leung, H B; Doering, Alice McGovern; Cohen, Seymour S.

doi:10.1128/jb.92.3.558-564.1966

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…This data is further supported by the lower cell number of ETEC after the co-culture. Similarly, di-peptide gln-gln involved in ETEC acid resistance [ 79 ] and arabinosylhypoxanthine involved in the purine metabolism, E. coli cellular growth, and virulence in mixed culture [ 80 , 81 ] were seen in higher abundance in the co-culture group. This may reflect an initial defensive response of ETEC to CP9 in the co-culture.…”

Section: Discussionmentioning

confidence: 99%

Potential Probiotic Bacillus subtilis Isolated from a Novel Niche Exhibits Broad Range Antibacterial Activity and Causes Virulence and Metabolic Dysregulation in Enterotoxic E. coli

et al. 2021

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Microbial life in extreme environments, such as deserts and deep oceans, is thought to have evolved to overcome constraints of nutrient availability, temperature, and suboptimal hygiene environments. Isolation of probiotic bacteria from such niche may provide a competitive edge over traditional probiotics. Here, we tested the survival, safety, and antimicrobial effect of a recently isolated and potential novel strain of Bacillus subtilis (CP9) from desert camel in vitro. Antimicrobial assays were performed via radial diffusion, agar spot, and co-culture assays. Cytotoxic analysis was performed using pig intestinal epithelial cells (IPEC-J2). Real time-PCR was performed for studying the effect on ETEC virulence genes and metabolomic analysis was performed using LC-MS. The results showed that CP9 cells were viable in varied bile salts and in low pH environments. CP9 showed no apparent cytotoxicity in IPEC-J2 cells. CP9 displayed significant bactericidal effect against Enterotoxic E. coli (ETEC), Salmonella Typhimurium, and Methicillin-resistant Staphylococcus aureus (MRSA) in a contact inhibitory fashion. CP9 reduced the expression of ETEC virulent genes during a 5 h co-culture. Additionally, a unique emergent metabolic signature in co-culture samples was observed by LC-MS analysis. Our findings indicate that CP9 exhibits a strong antibacterial property and reveals potential mechanisms behind.

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Section: Discussionmentioning

confidence: 99%

Potential Probiotic Bacillus subtilis Isolated from a Novel Niche Exhibits Broad Range Antibacterial Activity and Causes Virulence and Metabolic Dysregulation in Enterotoxic E. coli

et al. 2021

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“…The isolation of the polyauxotrophic E. coli strain 15 TAUAd, designated strain TAUAd, is described in the accompanying paper (4). The supplementation for growth of a mineral medium containing glucose with thymine, arginine, uracil, adenosine or adenine, and histidine is described in that paper, as are the growth and viability of the organism in various complete and deficient media.…”

Section: Methodsmentioning

confidence: 99%

“…Methods used for the study of the incorporation of thymine-2-C'4, arginine-guanido-C'4, and uracil-2-C" into strain TAUAd, as well as the sources of those compounds and of ara-A, were described (4). Streptomycin sulfate was obtained from Nutritional Biochemicals Corp., Cleveland, Ohio.…”

Section: Nh2mentioning

confidence: 99%

Polymer Synthesis in Killed Bacteria: Lethality of 2′,3′-Dideoxyadenosine

Doering¹,

Jansen²,

Cohen³

1966

Journal of Bacteriology

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Philadelphia), MEEK1 JANSEN, AND SEYMOUR S. COHEN. Polymer synthesis in killed bacteria: lethality of 2',3'-dideoxyadenosine. J. Bacteriol. 92:565-574. 1966.-We studied the metabolic capabilities of cells that had lost the ability to multiply under a variety of lethal treatments. Cultures of a polyauxotrophic mutant of Escherichia coli strain 15 requiring thymine, arginine, uracil, and adenine for growth were killed to a few per cent survivors by several different methods. These treatments included streptomycin, thymineless death, D-arabinosyladenine (ara-A), and 2',3'-dideoxyadenosine (DDA). The killed cells were washed and supplied with complete media, and were compared with control cells with respect to the ability to incorporate thymine, arginine, and uracil. Cells killed with streptomycin in the absence of thymine were only partially inhibited in deoxyribonucleic acid (DNA) synthesis; they were markedly inhibited in synthesis of ribonucleic acid (RNA) and protein. Cells that had suffered thymineless death were essentially uninhibited in DNA synthesis, partially so in RNA synthesis, but extensively inhibited in protein synthesis. Killing by ara-A did not prevent DNA synthesis, but markedly inhibited RNA and protein synthesis. The lethality of DDA was studied in the presence of exogenous adenosine; lethality was partially prevented by deoxyadenosine. Dideoxyadenosine was similar to ara-A and thymineless death in killing in a pattern in which RNA and protein synthesis continued while DNA synthesis was inhibited. Cells killed by DDA, however, were markedly inhibited in subsequent thymine incorporation, unlike cells killed by the other methods. In addition, at this time, the DDA-killed cells were more inhibited in incorporation of arginine than of uracil. DDA also potentiated thymineless death; when cells were killed rapidly by the combined treatment, only the ability to synthesize DNA was lost irreversibly. This agent (DDA) may permit the detailed study in E. coli of the relation of DNA synthesis to numerous phenomena, such as genetic recombination, sequential transcription, and the number and distribution of chromosome breaks.

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Antiproliferative Effects of 9-P-D-Arabinofuranosyladenine in a Mammalian Cell Line Devoid of Adenosine Deaminase Activity

1977

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The effect of ara-A on cellular growth, DNA synthesis, and RNA synthesis, and RNA synthesis was measured in an established cell line (B-mix K-44/6) devoid of adenosine deaminase activity. Cells adapted to growth in a medium supplemented with horse serum provided an environment totally lacking adenosine deaminase activity whereas cultivation of cells in a medium supplemented with calf serum provided a system capable of deaminating ara-A to ara-H (half-life = 14 hours). Under deaminase-free conditions early log phase cells underwent 1.5 population doublings during 28 hours compared with 0.25 doublings in the presence of 37 micronM ara-A. When cells were grown in medium supplemented with calf serum the additionof 37 to 225 micronM ara-A resulted in a cessation of mitosis for periods of 5 to 30 hours respectively. Following this quiescent period growth resumed at the original rate. With 600 micronM ara-A mitosis was reversibly inhibited up to 35 hours after drug addition. The effects of ara-A on RNA and DNA synthesis were monitored by continuously or pulse labeling B-mix K-44/6 cells with [3H]-uridine or [3H]thymidine. Ara-A did not influence RNA synthesis as judged by labeled uridine incorporation. Under deaminase-free conditions, 5.4 micronM ara-A inhibited labeled thymidine incorporation by 50%. In the presence of the enzyme, approximately twice the ara-A concentration was required for the same inhibition; furthermore the initial inhibition was followed by a partial recovery in the rate of thymidine incorporation. Examination of thymidine incorporation. Examination of thymidine nucleotide pools during ara-A treatment revealed to changes in the labeling of dTMP, dTDP, and dTTP. Thus inhibition of [3H]thymidine incorporation by ara-A accurately reflected inhibition of DNA synthesis. We conclude that, in spite of an initial inhibition of DNA synthesis and mitosis by ara-A, B-mix K-44/6 cells recover from the inhibitory effects if the drug is removed either by a change in the culture medium or by metabolism to ara-H.

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Effect of 9-β- d -Arabinofuranosyladenine on Polymer Synthesis in a Polyauxotrophic Strain of Escherichia coli

Cited by 3 publications

References 11 publications

Potential Probiotic Bacillus subtilis Isolated from a Novel Niche Exhibits Broad Range Antibacterial Activity and Causes Virulence and Metabolic Dysregulation in Enterotoxic E. coli

Potential Probiotic Bacillus subtilis Isolated from a Novel Niche Exhibits Broad Range Antibacterial Activity and Causes Virulence and Metabolic Dysregulation in Enterotoxic E. coli

Polymer Synthesis in Killed Bacteria: Lethality of 2′,3′-Dideoxyadenosine

Antiproliferative Effects of 9-P-D-Arabinofuranosyladenine in a Mammalian Cell Line Devoid of Adenosine Deaminase Activity

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