Effect of 60 and 90 days of isotretinoin treatment on the structure of the small intestine mucosa in young male Wistar rats

Abstract

Isotretinoin is a substance used in cases of severe acne and acne resistant to other treatments. This skin disease affects patients of all ages and can interfere with social life, especially in adolescents. The drug acts by suppressing sebaceous gland activity and creating an inhospitable environment for Propionibacterium acne. The integrity of the small intestine is important for correct nutrition and patient treatment. We intended to assess the small intestine structure after treatment with 5 mg/kg isotretinoin solution and after a period without the drug, which could be considered a rest period. Young male Wistar rats (n=24) were separated into 4 groups (n=6): C: water; D0: soybean oil; D5a: 5 mg/kg; D5b: 5 mg/kg for 60 days followed by 30 days of rest period. Soybean oil was used to dilute the drug and it was offered daily by gavage. The animals were euthanized and the duodenum, jejunum and ileum were collected for analysis with light and scanning electron microscopy. The treatment stimulated tissue proliferation in the jejunum and ileum but had no significant effect in the duodenum. The results also showed a modification in goblet cell frequency in the duodenum and ileum. A further finding was that some modifications disappeared during the rest period. The protocol showed that the small intestine was somewhat altered by the treatment yet no lasting damage was caused.

Preparation a core‐shell lipid/polymer nanoparticle containing Isotretinoin drug with pH sensitive property: A response surface methodology study

“…Therefore, its release before reaching intestine must be prevented. 21 Given that pH of the gastrointestinal tract is considerably different, the pH-sensitive carriers can offer a promising tool for controlled drug release in the oral administration of Isotretinoin. 22 Polymeric nanoparticles and lipid/polymeric hybrid nanomaterials can be useful for this purpose.…”