Effect of 5-aza-2′-deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos

Zhao, Xiao‐Fan; Ren, Jingjing; Du, Weihua; Huang, Hao; Wang, Dong; Yan, Liying; Tong, Qin; Zhu, Huabin

doi:10.1016/j.fertnstert.2012.04.014

Cited by 23 publications

(14 citation statements)

References 34 publications

(58 reference statements)

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“…In contrast, vitrification has been found to reduce gene methylation in mouse oocytes [113], embryos [112], and fetuses [117]. However, other studies have demonstrated that vitrification significantly increases gene methylation in mouse oocytes [116] as well as bovine two cell embryos and the resulting in vitro-derived blastocysts [111]. Regarding the effects of vitrification on acetylation patterns, several studies have indicated that vitrification significantly alters acetylation patterns in oocytes [92,114,116,118,120].…”

Section: Molecular Effects Of Cryopreservationmentioning

confidence: 99%

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Moussa

Shen

Zhang

et al. 2014

Sci. China Life Sci.

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Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades, emphasizing their importance in various assisted reproductive technologies. Pregnancies and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro fertilization. Considerable progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos, including preservation of the reproductive potential of patients who may become infertile, establishment of cryopreserved oocyte banks, and transport of oocytes and embryos internationally. However, the success rates are still far lower than those obtained with fresh oocytes and embryos, and there are still obstacles that need to be overcome. In this review, we address the major obstacles in the development of effective cryopreservation techniques. Such knowledge may help to eliminate these hurdles by revealing which aspects need improvement. Furthermore, this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species. cryopreservation, mammals, oocytes, embryos, cryoinjuries Citation:Moussa M, Shu J, Zhang XH, Zeng FY. Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives. Sci China Life Sci, 2014, 57: 903 -914,

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Section: Molecular Effects Of Cryopreservationmentioning

confidence: 99%

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Moussa

Shen

Zhang

et al. 2014

Sci. China Life Sci.

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“…sFlt-1 binds VEGF and thereby reduces its free circulating level. Expression of sFlt-1 is upregulated in cytotrophoblasts during hypoxia and is suggested to induce endothelial damage (Nagamatsu et al, 2004; Zhao et al, 2012). sFlt-1 expression is increased in pre-eclampsia and evidence suggests that it causes the development of the disease (Shibata et al, 2005; Zhao et al, 2012).…”

Section: Alterations In Polyadenylation In Endocrine Diseasementioning

confidence: 99%

Alterations in Polyadenylation and Its Implications for Endocrine Disease

Rehfeld¹,

Plass²,

Krogh³

et al. 2013

Front. Endocrinol.

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Introduction: Polyadenylation is the process in which the pre-mRNA is cleaved at the poly(A) site and a poly(A) tail is added – a process necessary for normal mRNA formation. Genes with multiple poly(A) sites can undergo alternative polyadenylation (APA), producing distinct mRNA isoforms with different 3′ untranslated regions (3′ UTRs) and in some cases different coding regions. Two thirds of all human genes undergo APA. The efficiency of the polyadenylation process regulates gene expression and APA plays an important part in post-transcriptional regulation, as the 3′ UTR contains various cis-elements associated with post-transcriptional regulation, such as target sites for micro-RNAs and RNA-binding proteins.Implications of alterations in polyadenylation for endocrine disease: Alterations in polyadenylation have been found to be causative of neonatal diabetes and IPEX (immune dysfunction, polyendocrinopathy, enteropathy, X-linked) and to be associated with type I and II diabetes, pre-eclampsia, fragile X-associated premature ovarian insufficiency, ectopic Cushing syndrome, and many cancer diseases, including several types of endocrine tumor diseases.Perspectives: Recent developments in high-throughput sequencing have made it possible to characterize polyadenylation genome-wide. Antisense elements inhibiting or enhancing specific poly(A) site usage can induce desired alterations in polyadenylation, and thus hold the promise of new therapeutic approaches.Summary: This review gives a detailed description of alterations in polyadenylation in endocrine disease, an overview of the current literature on polyadenylation and summarizes the clinical implications of the current state of research in this field.

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“…In this issue of Fertility and Sterility, Zhao et al (1) have found that H19 putative imprinted control region (ICR) level of methylation significantly increased in vitrified two-cell bovine embryos and the generated blastocysts. They have also observed that incubation of vitrified-thawed 2-cell embryos with the DNA methyltransferase inhibitor 5-aza-2 0 -deoxycytidine (5-aza-dC) significantly reduced H19 ICR methylation and increased H19 expression by reverse transcription-polymerase chain reaction in the blastocysts derived from the vitrified embryos compared with those in fresh embryos.…”

mentioning

confidence: 99%

“…However, during the early stages of embryogenesis, both genomes undergo remethylation until the blastocyst stage (1, 2). Although the possible effect(s) of ART/IVF and cryopreservation on DNA methylation has been the focus of numerous publications, the possible effects remain equivocal (1,3). Such a putative effect is of utmost importance owing to the translatable extrapolation on safety concerns for ART/IVF-generated neonates.…”

mentioning

confidence: 99%

“…As Zhao et al (1) correctly cite, the H19 putative imprinted control region regulates embryogenesis and fetoplacental differentiation through expression of IGF2 and may be sensitive to COS and in vitro gamete and embryo manipulation, possibly leading to diseases of dominant inheritance in ART/IVF neonates. The authors explain that since de novo methylation usually occurs at the 8-to 16-cell stage in bovine embryos, they cultured the vitrified-thawed 2-cell embryos with 5-aza-dC, since such exposure before the 8-cell stage embryo may inhibit premature de novo methylation without interrupting new methylation patterns.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Epigenetics and vitrification of embryos: one step forward and many raised equivocal questions

Blumenfeld¹

2012

Fertility and Sterility

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Effect of 5-aza-2′-deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos

Cited by 23 publications

References 34 publications

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Alterations in Polyadenylation and Its Implications for Endocrine Disease

Epigenetics and vitrification of embryos: one step forward and many raised equivocal questions

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