Effect of 48-h Food Deprivation on the Expressions of Myosin Heavy-Chain Isoforms and Fiber Type-Related Factors in Rats

Mizunoya, Wataru; Sawano, Shoko; Iwamoto, Yuka; Sato, Yusuke; Tatsumi, Ryuichi; Ikeuchi, Yoshihide

doi:10.3177/jnsv.59.289

Cited by 6 publications

(3 citation statements)

References 56 publications

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“…Thus, initial conditions may have been similar to the soybean oil-fed group. Previously, we reported the effects of 48-h food deprivation on muscle fiber type properties [ 50 ].. In that study, rats of the same strain, age, breeder, and commercial diet (CRF-1) were used and MyHC composition of the control group was very similar to this experiment in both EDL and soleus muscle.…”

Section: Discussionmentioning

confidence: 95%

Dietary Fat Influences the Expression of Contractile and Metabolic Genes in Rat Skeletal Muscle

et al. 2013

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Dietary fat plays a major role in obesity, lipid metabolism, and cardiovascular diseases. To determine whether the intake of different types of dietary fats affect the muscle fiber types that govern the metabolic and contractile properties of the skeletal muscle, we fed male Wistar rats with a 15% fat diet derived from different fat sources. Diets composed of soybean oil (n-6 polyunsaturated fatty acids (PUFA)-rich), fish oil (n-3 PUFA-rich), or lard (low in PUFAs) were administered to the rats for 4 weeks. Myosin heavy chain (MyHC) isoforms were used as biomarkers to delineate the skeletal muscle fiber types. Compared with soybean oil intake, fish oil intake showed significantly lower levels of the fast-type MyHC2B and higher levels of the intermediate-type MyHC2X composition in the extensor digitorum longus (EDL) muscle, which is a fast-type dominant muscle. Concomitantly, MyHC2X mRNA levels in fish oil-fed rats were significantly higher than those observed in the soybean oil-fed rats. The MyHC isoform composition in the lard-fed rats was an intermediate between that of the fish oil and soybean oil-fed rats. Mitochondrial uncoupling protein 3, pyruvate dehydrogenase kinase 4, and porin mRNA showed significantly upregulated levels in the EDL of fish oil-fed rats compared to those observed in soybean oil-fed and lard-fed rats, implying an activation of oxidative metabolism. In contrast, no changes in the composition of MyHC isoforms was observed in the soleus muscle, which is a slow-type dominant muscle. Fatty acid composition in the serum and the muscle was significantly influenced by the type of dietary fat consumed. In conclusion, dietary fat affects the expression of genes related to the contractile and metabolic properties in the fast-type dominant skeletal muscle, where the activation of oxidative metabolism is more pronounced after fish oil intake than that after soybean oil intake.

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Section: Discussionmentioning

confidence: 95%

Dietary Fat Influences the Expression of Contractile and Metabolic Genes in Rat Skeletal Muscle

et al. 2013

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“…Fasting, however, should have little effect on the expression of type I MyHC isoforms ( Mizunoya et al , 2013 ). Instead, the decreased expression by October ice bears indicates that summer atrophy was also at least partially caused by reduced activity.…”

Section: Discussionmentioning

confidence: 99%

Polar bears experience skeletal muscle atrophy in response to food deprivation and reduced activity in winter and summer

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et al. 2017

Conservation Physiology

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“…In addition the glycolytic fibers were selectively decreased in the cross sectional area lead to a proportional increase in the area of oxidative fibers in bovine muscle (Greenwood et al, 2009). Muscle phenotype also changed according to the dietary challenges, It has been reported that dietary deprivation for 48 hours upregulates the expression of fast myosin heavy chain 2b mRNA with no change in fiber type composition for EDL and soleus muscles in rat (Mizunoya et al, 2013b) However 4 weeks fatty diet administration induced reduction in the fast MHC2b and improved the oxidative metabolism in the EDL of rat (Matsakas et al, 2013;Mizunoya et al, 2013a). Previously, it was shown that fasting induces muscle proteolysis via glucocorticoid activation (Wing and Goldberg, 1993) as well as causing an increase in the ATP-dependent proteolysis and upregulation of ubiquitin conjugates and polyubiquitins in rat skeletal muscle (Medina et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

The effect of caloric restriction on the forelimb skeletal muscle fibers of the hypertrophic myostatin null mice

et al. 2017

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Skeletal muscle mass loss has a broad impact on body performance and physical activity. Muscle wasting occurs due to genetic mutation as in muscular dystrophy, age-related muscle loss (sarcopenia) as well as in chronic wasting disorders as in cancer cachexia. Food restriction reduces muscle mass underpinned by increased muscle protein break down. However the influence of dietary restriction on the morphometry and phenotype of forelimb muscles in a genetically modified myostatin null mice are not fully characterized. The effect of a five week dietary limitation on five anatomically and structurally different forelimb muscles was examined. C57/BL6 wild type (Mstn) and myostatin null (Mstn) mice were either given a standard rodent normal daily diet ad libitum (ND) or 60% food restriction (FR) for a 5 week period. M. triceps brachii Caput laterale (T.lateral), M. triceps brachii Caput longum (T.long), M. triceps brachii Caput mediale (T.medial), M. extensor carpi ulnaris (ECU) and M. flexor carpi ulnaris (FCU) were dissected, weighted and processed for immunohistochemistry. Muscle mass, fibers cross sectional areas (CSA) and myosin heavy chain types IIB, IIX, IIA and type I were analyzed. We provide evidence that caloric restriction results in muscle specific weight reduction with the fast myofibers being more prone to atrophy. We show that slow fibers are less liable to dietary restriction induced muscle atrophy. The effect of dietary restriction was more pronounced in Mstn muscles to implicate the oxidative fibers compared to Mstn. Furthermore, peripherally located myofibers are more susceptible to dietary induced reduction compared to deep fibers. We additionally report that dietary restriction alters the glycolytic phenotype of the Mstn into the oxidative form in a muscle dependent manner. In summary our study shows that calorie restriction alters muscle fiber profile of forelimb muscles of Myostatin null mice.

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Effect of 48-h Food Deprivation on the Expressions of Myosin Heavy-Chain Isoforms and Fiber Type-Related Factors in Rats

Cited by 6 publications

References 56 publications

Dietary Fat Influences the Expression of Contractile and Metabolic Genes in Rat Skeletal Muscle

Dietary Fat Influences the Expression of Contractile and Metabolic Genes in Rat Skeletal Muscle

Polar bears experience skeletal muscle atrophy in response to food deprivation and reduced activity in winter and summer

The effect of caloric restriction on the forelimb skeletal muscle fibers of the hypertrophic myostatin null mice

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