Effect of 45nm silver nanoparticles (AgNPs) upon the smooth muscle of rat trachea: Role of nitric oxide

González, Carmen; Salazar-García, Samuel; Palestino, Gabriela; Martinez-Cuevas, Pedro Pablo; Ramirez-Lee, Manuel Alejandro; Jurado-Manzano, Brenda B.; Rosas-Hernández, Héctor; Gaytán-Pacheco, Noemí; Martel, Guadalupe; Espinosa‐Tanguma, Ricardo; Biris, Alexandru S.; Ali, Syed F.

doi:10.1016/j.toxlet.2011.09.024

Cited by 23 publications

(13 citation statements)

References 31 publications

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“…Thus, our data suggested that 2.6 and 18 nm AgNPs regulated NOS protein levels by different pathways: 2.6 nm AgNPs regulates at the transcriptional level, whereas 18 nm AgNPs regulates at the translational level. Similarly, Niu et al [ 38 ] described 1–10 nm CeO2-induced increase in iNOS mRNA level in human cardiomyocytes, and González et al [ 39 ] showed that 45 nm AgNPs induced increase in iNOS protein level in the rat tracheal smooth muscle. Moreover, Xue et al [ 40 ] compared the induction of protein and mRNA iNOS level depending on the type and size of NP in primary microglia cells.…”

Section: Discussionmentioning

confidence: 96%

Role of Oxidative and Nitro‐Oxidative Damage in Silver Nanoparticles Cytotoxic Effect against Human Pancreatic Ductal Adenocarcinoma Cells

Barcińska

Wierzbicka

Zauszkiewicz-Pawlak

et al. 2018

Oxidative Medicine and Cellular Longevity

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Pancreatic ductal adenocarcinoma is one of the most aggressive human malignancies, where the 5-year survival rate is less than 4% worldwide. Successful treatment of pancreatic cancer is a challenge for today's oncology. Several studies showed that increased levels of oxidative stress may cause cancer cells damage and death. Therefore, we hypothesized that oxidative as well as nitro-oxidative stress is one of the mechanisms inducing pancreatic cancer programmed cell death. We decided to use silver nanoparticles (AgNPs) (2.6 and 18 nm) as a key factor triggering the reactive oxygen species (ROS) and reactive nitrogen species (RNS) in pancreatic ductal adenocarcinoma cells (PANC-1). Previously, we have found that AgNPs induced PANC-1 cells death. Furthermore, it is known that AgNPs may induce an accumulation of ROS and alteration of antioxidant systems in different type of tumors, and they are indicated as promising agents for cancer therapy. Then, the aim of our study was to evaluate the implication of oxidative and nitro-oxidative stress in this cytotoxic effect of AgNPs against PANC-1 cells. We determined AgNP-induced increase of ROS level in PANC-1 cells and pancreatic noncancer cell (hTERT-HPNE) for comparison purposes. We found that the increase was lower in noncancer cells. Reduction of mitochondrial membrane potential and changes in the cell cycle were also observed. Additionally, we determined the increase in RNS level: nitric oxide (NO) and nitric dioxide (NO2) in PANC-1 cells, together with increase in family of nitric oxide synthases (iNOS, eNOS, and nNOS) at protein and mRNA level. Disturbance of antioxidant enzymes: superoxide dismutase (SOD1, SOD2, and SOD3), glutathione peroxidase (GPX-4) and catalase (CAT) were proved at protein and mRNA level. Moreover, we showed cells ultrastructural changes, characteristic for oxidative damage. Summarizing, oxidative and nitro-oxidative stress and mitochondrial disruption are implicated in AgNPs-mediated death in human pancreatic ductal adenocarcinoma cells.

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Section: Discussionmentioning

confidence: 96%

Role of Oxidative and Nitro‐Oxidative Damage in Silver Nanoparticles Cytotoxic Effect against Human Pancreatic Ductal Adenocarcinoma Cells

Barcińska

Wierzbicka

Zauszkiewicz-Pawlak

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…Different morphological structures of the NP promote neuron injuries, alter the activity of some glial cells and the depletion of myelin fibre due to the elevated activity of heat shock proteins (HSPs) [11]. The action of acetylcholine was affected due to increased nitric acid production when rats were treated with Ag‐NP of size 45 nm, which also induces hyperactivity of the tracheal smooth muscles [15]. It was observed that the PC‐12 cell line (cells obtained from a pheochromocytoma of the rat adrenal medulla) showed the deterioration of membrane structure and cell shrinkage [16] when treated with Ag‐NP.…”

Section: Effects Of Np On Animalsmentioning

confidence: 99%

Promising opportunities and potential risk of nanoparticle on the society

Dash

Kundu

2020

IET nanobiotechnol.

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The ever-promising opportunities and the uses of NP in our life are increasing but their present and future potential risks on the animals, plants and microorganisms are not well discussed elsewhere. In this review, the authors have systematically discussed the toxic effect of the uses of NP on animals, plants and microorganisms including human health. They have also discussed about the bioaccumulation of these NP in the food chain. Finally, they have provided some possible suggestions for the uses of NP to reduce the detrimental effect on the environment.

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“…Silver nanoparticles also observed to have destructive effects on pigs [38]. Even in rats it was found to have induction of the hyperactivity of the tracheal smooth muscle by the action of acetylcholine with a production of NO (nitric oxide) @25nm [39]. After the investigation of the various organs post-exposure to silver nanoparticles, there traces were observed in spleen red pulp, lungs, and kidneys in mouse [40].…”

Section: Mammalsmentioning

confidence: 99%

Consequences of Nanoparticle - Insecticides on Aquatic and Terrestrial Non - Target Organisms: A Review

Riat¹

2018

IZAB

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Insects create great nuisance in different manners and spread various diseases. Mosquitoes are arthropods of medical and veterinary importance as they are the vectors of deadly diseases like malaria, dengue and Zika virus. Many different methods are adopted at various parts of the world to control mosquitoes ae well as other insect pest. But challenges are faced with the adoption of such measures, which leads to resistance of target organism and harmful effects to non-target organisms. Bio-pesticides as well as nanotechnology is considered a potential eco-friendly approach in mosquito control research. Plant-mediated nanotechnology is current most and effective method to control the population of mosquitoes. Furthermore, it is analyzed from the various evidences of ill-effects of nanocomposites to the non-targets organisms including mammals, humans, and aquatic insects.

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Effect of 45nm silver nanoparticles (AgNPs) upon the smooth muscle of rat trachea: Role of nitric oxide

Cited by 23 publications

References 31 publications

Role of Oxidative and Nitro‐Oxidative Damage in Silver Nanoparticles Cytotoxic Effect against Human Pancreatic Ductal Adenocarcinoma Cells

Role of Oxidative and Nitro‐Oxidative Damage in Silver Nanoparticles Cytotoxic Effect against Human Pancreatic Ductal Adenocarcinoma Cells

Promising opportunities and potential risk of nanoparticle on the society

Consequences of Nanoparticle - Insecticides on Aquatic and Terrestrial Non - Target Organisms: A Review

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