Effect of 2-mercaptoethanol on some metabolic indices of ageing of CBA/Ca inbred mice

Pénzes, L; Noble, R. C.; Beregi, E; Imre, S.; Izsák, J; Regius, O

doi:10.1016/0047-6374(88)90021-8

Cited by 13 publications

(5 citation statements)

References 34 publications

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“…Microgram quantities of β‐ME have demonstrated to have a number of possibly beneficial effects on experimental animals. It has been found in several studies that β‐ME improves the life span and other aspects of life performance in laboratory animals (Penzes et al . 1988).…”

Section: Discussionmentioning

confidence: 99%

Biochemical Characterization and Thermostable Capacity of Parvalbumins: The Major Fish-Food Allergens

Arif¹,

Jabeen

Hasnain

2007

J Food Biochemistry

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The major allergens in fish‐food are parvalbumins (PVs). Muscle PVs of four fish species of genus Channa: Channa gachua, Channa punctatus, Channa striatus and Channa marulius were purified, characterized and used to differentiate (heated) fish specimens. Two to three proteins have been detected as PV‐isoforms by immunological probing through Western blotting. They were purified to homogeneity on diethyl amino ethyl‐cellulose. Modified systems of nondenaturing and denaturing polyacrylamide gel electrophoresis were used to resolve low molecular weight proteins, distinctly. Isoelectric points (pIs) were determined on native isoelectric focusing gels, which lie in the pI range of 4.55–5.10. The electrophoretic profiles remained species‐specific even after heating the PVs for 3 h at 90C and would be of immense help in discerning cooked fish specimens. Further, the addition of 5 mM β‐mercaptoethanol in the PVs has showed protection against heat denaturation of the protein and resulted in more compatible protein patterns. This study also demonstrates the relevance of the thermostable capacity of PVs in the differentiation of heat‐processed fish through electrophoretic analysis of heated PVs. Also, they represent a promising and simple biochemical tool in discerning closely related species.

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Section: Discussionmentioning

confidence: 99%

Biochemical Characterization and Thermostable Capacity of Parvalbumins: The Major Fish-Food Allergens

Arif¹,

Jabeen

Hasnain

2007

J Food Biochemistry

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“…And lastly, 3 independent laboratories described alteration of spontaneous occurring cancers via undefined etiology agents by daily continuous exposure to 2-Me [23,24,64,151]. In the first report [23], 16 week old (C57BL × C3H/Anf) F 1 male mice were fed a diet either free of or containing 0.25% (w/w) 2-Me (this calculates to a daily consumption of ca.…”

Section: Resultsmentioning

confidence: 99%

“…It is important to note that neither of these latter processes were prevented, they were simply delayed. The second study, with a different strain of mice, CBA/Ca also found that diseases associated with old-age were slowed [24,151]. The mice consumed considerably less (8 ugm daily) from their water starting at 5 months of age and continued until they were terminated at 20 months (610 days).…”

Section: Resultsmentioning

confidence: 99%

Anticancer activity and chemoprevention of xenobiotic organosulfurs in preclinical model systems

Click¹

2013

Oncol Discov

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There seems to be little doubt that xenobiotic and plant derived organosulfur compounds have enormous benefits for in vitro cellular functions and for a multitude of diseases, including cancer. Since there are numerous reviews on anticancer activities of plant organosulfurs, the focus herein will be on alterations associated with xenobiotic organosulfurs. Benefits of 2-mercaptoethanol (2-Me), N-Acetyl-cysteine, cysteamine, thioproline, piroxicam, disulfiram, amifostine, sulindac, celecoxib, oltipraz and their derivates on transplanted homologous tumors and on autochthonous cancers with a viral-, radiation-, chemical carcinogen-, and undefined-etiology are assessed. Because all organosulfurs were not tested for activity in each of the etiology categories, comparative evaluations are restricted. In general, all ‘appeared’ to lower the incidence of cancer irrespective of etiology; however, since most of these values were determined at ages much younger than at a natural-end-of-life-age, differences most likely, instead, reflect a delayed initiation and/or a slowed progression of tumorigenesis. The poorest, long-term benefits of early intervention protocols occurred for viral- and chemical carcinogen-induced cancers. In addition, once tumorigenesis was beyond the initiation stage, outcomes of organosulfur therapies were extremely poor, indicating that they will not be of significant value as stand alone treatments. More importantly, except for the lifetime prevention of spontaneous and radiation-induced mammary tumors by daily dietary 2-Me, similar life long prevention of tumorigenesis was not achieved with other xenobiotics or any of nature’s plant organosulfurs. These results raise an interesting question: Is the variability in incidence found for different organosulfurs associated with (a) their structure, (b) the length of the untreated latency period, (c) treatment duration/dose, and/or (d) the etiology-inducing agent?

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“…Supplementation with 2-Me was well-tolerated as it did not alter consumption of food or water and did not result in weight loss/gain compared with age-matched controls-these data are not included since it did not differ from that reported previously for many other strains. [18][19][20][21]31,40 Treatment with 2-Me was started at weaning (35-40 d of age) and continued for an animal's entire life. Average daily consumption of 2-Me fluctuated over the lifetime from 60-80 ugm/gm body wt.…”

Section: Bxsb-yaamentioning

confidence: 99%

“…Research on xenobiotic sulfur compounds with simple structures has generally been focused on in vitro benefits for a multitude of cell-types and processes (> 1,000 articles in PubMed). Subsequently, as might be expected, xenobiotics (primarily 2-Me and cysteamine) when either injected into or fed to animals were found to modify a multitude of disease processes (REC in preparation), including spontaneous tumor development [18][19][20] and virally-, 21,22 carcinogenic- [23][24][25][26][27][28] and radiation-induced cancers. [29][30][31] The present report describes alteration of spontaneous and virally-induced tumor development by the extensively investigated immune enhancing, xenobiotic organosulfur, 2-Me and compares its anticancer efficacy to that of other organosulfurs.…”

Section: Introductionmentioning

confidence: 99%

Dietary supplemented 2-mercaptoethanol prevents spontaneous and delays virally-induced murine mammary tumorigenesis

Click

2013

Cancer Biology & Therapy

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Effect of 2-mercaptoethanol on some metabolic indices of ageing of CBA/Ca inbred mice

Cited by 13 publications

References 34 publications

Biochemical Characterization and Thermostable Capacity of Parvalbumins: The Major Fish-Food Allergens

Biochemical Characterization and Thermostable Capacity of Parvalbumins: The Major Fish-Food Allergens

Anticancer activity and chemoprevention of xenobiotic organosulfurs in preclinical model systems

Dietary supplemented 2-mercaptoethanol prevents spontaneous and delays virally-induced murine mammary tumorigenesis

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