Effect of 2-deoxy-d-Glucose on Aminoacids Metabolism in Rats’ Cerebral Cortex Slices

Abstract

We studied the effect of different concentrations of 2-deoxy-D-glucose on the L-[U-14C]leucine, L-[1-14C]leucine and [1-14C]glycine metabolism in slices of cerebral cortex of 10-day-old rats. 2-deoxy-D-glucose since 0.5 mM concentration has inhibited significantly the protein synthesis from L-[U-14C]leucine and from [1-14C]glycine in relation to the medium containing only Krebs Ringer bicarbonate. Potassium 8.0 mM in incubation medium did not stimulate the protein synthesis compared to the medium containing 2.7 mM, and at 50 mM diminishes more than 2.5 times the protein synthesis compared to the other concentration. Only at the concentration of 5.0 mM, 2-deoxy-D-glucose inhibited the CO2 production and lipid synthesis from L-[U-14C] leucine. This compound did not inhibit either CO2 production, or lipid synthesis from [1-14C]glycine. Lactate at 10 mM and glucose 5.0 mM did not revert the inhibitory effect of 2-deoxy-D-glucose on the protein synthesis from L-[U-14C]leucine. 2-deoxy-D-glucose at 2.0 mM did not show any effect either on CO2 production, or on lipid synthesis from L-[U-14C]lactate 10 mM and glucose 5.0 mM.

Propylthiouracil-induced hypothyroidism during lactation alters leucine and mannose metabolism in rat cerebellar slices

“…The flask contents were homogenized and transferred to tubes. 28 Lipid and protein synthesis After centrifugation, the precipitate was washed 3 times with (1 mL) 10% TCA, and lipids were extracted with chloroform/methanol (2:1). The chloroform/methanol phase was evaporated in vials and radioactivity was measured.…”

Can Reducing Sugar Retard Kindling?

“…For example, reduced glycolytic flux might lead to compensatory mitochondrial biogenesis and an increase in energy reserve (10) initiated in astrocytes, which are the predominant locus of glycolysis in the brain. Also, potentially relevant to 2‐DG antiepileptogenic actions, Muller et al recently reported that 2‐DG inhibits protein synthesis in immature rodent brain (11). Finally, 2‐DG may have a different profile of activity in conventional seizure models than does the KD (12), suggesting that it is unlikely to truly replace the KD.…”