Effect of 2-Bromoethanesulfonic Acid on In vitro Fermentation Characteristics and Methanogen Population

Reducing in vitro rumen methanogenesis for two contrasting diets using a series of inclusion rates of different additives

“…The extent of reduction in methane output with increasing concentration of BES was more pronounced for GS+B than for ryegrass. This is in agreement with Lee et al (2009), who found a similar interaction for in vitro rumen methane output between feed type (timothy hay vs timothy hay+concentrate, 40 : 60) and the level of BES supplementation (0, 1 and 5 mM). In the present study, the greater reduction in methane output for GS+B with the addition of BES was not due to differences in the extent of fermentation as measured by aDMd or tVFA/DMi, but to the direction of fermentation with a numerically lower A : P ratio (data not presented) for GS+B than for ryegrass.…”

“…Goel et al (2009) reported the reduction of in vitro methane output using 5 mM BCM with hay as substrate, compared with between 1 and 5 mM BCM required for ryegrass and GS+B in the present study. In the case of BES, 1 mM significantly reduced methane output in timothy hay and timothy hay+concentrate (40 : 60) diets (Lee et al 2009), which was similar for GS+B in the present study, but not for ryegrass, which required 5-10 mM BES to significantly reduce methane output. There were no comparative studies in the literature for PMDI.…”

Effects of supplementing a mixed diet with echium (<i>Echium plantagineum</i>) oil on methanogenesis in a rumen simulation system

“…Methane production was only detected on day 3 of the experimental period for the BES treatment (data not shown), as earlier observed by Karnati et al (2009) with 250 µM of BES, and suggesting an adaptation of the methanogenic population to the halogenated compound. Sodium 2-bromoethanesulfonate reduced methane production by 92.8%, compared to the control, a similar finding (95%) being described for the same supplementation level in vitro (Lee et al, 2009).…”

Effects of 3-nitrooxypropanol on methane production using the rumen simulation technique (Rusitec)

“…Typically, the use of NOP (Haisan et al, 2014;Martínez-Fernández et al, 2014;Romero-Perez et al, 2014, 2015 and other CH 4 inhibitors like BCM (McCrabb et al, 1997), BES (Lee et al, 2009;O'Brien et al, 2014) and chloroform (Knight et al, 2011) has reduced the rumen molar proportion of acetate and increased the molar proportion of propionate with concomitant reduction in the acetate to propionate ratio. When CH 4 production is inhibited, an increase in propionate production is expected because propionate is considered the most important hydrogen sink in the rumen after CH 4 (McAllister and Newbold, 2008).…”