Effect of 2,4-Dichlorophenoxyacetic Acid on Proteolytic Activity of Red Kidney Bean Plants

Rebstock, Theodore L.; Hamner, C. L.; Ball, Charles D.; Sell, Harold M.

doi:10.1104/pp.27.3.639

Cited by 8 publications

(4 citation statements)

References 1 publication

(1 reference statement)

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“…Present techniques can be expected to give only relative values. Nevertheless, effects of 2,4-D on proteolytic activity in extracts of leaves and stems in our experiments are essentially the same as those of Rebstock et al (11) whose study differed from ours in species treated, technique of exposure to 2,4-D, method of drying the tissue, and technique of measuring proteolytic activity.…”

Section: Discussionsupporting

confidence: 89%

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Changes in Nitrogen Fractions and Proteolytic Enzymes of Soybean Plants Treated with 2,4-Dichlorophenoxyacetic Acid.

Freiberg¹,

Clark²

1955

Plant Physiol.

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Section: Discussionsupporting

confidence: 89%

“…Rebstock et al (11), working with red kidney bean seedlings after application of 2,4-D to the primary leaf, found greater proteolytio activity in 2,4-D treated than in control stems, but lower proteolytic activity in treated than in control leaves.…”

mentioning

confidence: 98%

Changes in Nitrogen Fractions and Proteolytic Enzymes of Soybean Plants Treated with 2,4-Dichlorophenoxyacetic Acid.

Freiberg¹,

Clark²

1955

Plant Physiol.

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“…Rebstock et al (6) and Freiburg (4) found that 2,4-D caused increases in proteinase and polypeptidase activities which lends some support to these findings. Carbohydrate and sugar reserves in bean plants are readily depleted following 2,4-D treatment, as reported by several workers (7, S, 9) and the synthesis of aspartic and glutamic acids is increased as indi-cate(l by this work.…”

supporting

confidence: 65%

Studies in Plant Metabolism. VI. Effect of 2,4-D on the Metabolism of Aspartic Acid and Glutamic Acid in the Bean Plant.

Akers

Fang²

1956

Plant Physiol.

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In studying the effect of 2,4-D treatment on the chemical composition of bean plants, Sell et al (7) reported that the stem of 2,4-D treated plants contained almost twice as much crude protein as do stems of control plants. Later, Weller et al (9) found that 2,4-D treatment caused a slight decrease in crude leaf protein while the crude root protein remained approximately the same. The amino acid composition in the crude protein from 2,4-D treated stems were different from those of the control plants. A small difference was noted in the leaf and root proteins. These data suggested to the authors that a change had occurred in the character of the protein. Using potato tubers, Payne et al (5) found that 2,4-D caused an increase in free glutamic acid and a decrease in eleven amino acids including aspartic acid. The decrease was explained as an increased catabolism of the free amino acids. In a preliminary investigation on the effects of 2,4-D 2,4,5-T and indoleacetic acid treatment, we have found that the amounts of total aspartic and glutamic acids decreased in both the stems and roots of 2,4-D and 2,4,5-T treated plants, with the greatest change occurring in the stems. Two possible explanations may be given: 1) the 2,4-D treatment might inhibit the synthesis of these amino acids or 2) this treatment nmight increase the rate of oxidation of the amino acids.In order to clarify this point, the present investigation, using carbon 14 as a tracer to study the rates of synthesis of both glutamic acid and aspartic acid in 2,4-D treated and control plants was figure 1. This system consisted of a bell jar large enough to contain one set of four plants, a column of calcium chloride to absorb the excess moisture, a generator to produce radioactive CO2 from BaC1403 after the system was sealed, a Geiger-Muller tube to detect the radioactivity, and a pump to circulate the atmosphere.One day after treatment, the first set of bean plants was placed in the closed system and exposed to an atmosphere containing C1402 for a period of 12 hours in light and 12 hours in darkness. Two 40-watt fluorescent lamps which have a light intensity of 200 fc were set 6 inches from the bell jar on opposite sides. The radioactivity readings of the atmosphere were taken periodically in order to check the amount of radioactivity absorbed by the plants.The second and third sets of bean plants were exposed to C1402 in a similar manner, three days and seven days, respectively, after the treatment with 2,4-D. The amounts of BaC1403 used for these three experiments were 4.3 mg, 6.3 mg, and 5.1 mg respectively (20.6 pxc/mg).After exposure to radioactive C02, the entire plants were harvested and dried overnight at 550 C in a vacuum oven. The samples were then weighed, ground and stored in an evacuated desiccator.RADIOACTIVITY M1EASUREMENTS: Weiglhed aliquots of each sample were oxidized by dry combustion (2). The carbon dioxide was quantitatively absorbed in carbonate-free sodium hydroxide solution and precipitated as barium carbonate. The radioactivit...

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“…Sell et al, 1949), possibly at the expense of the levels in leaves and roots (Weller et al, 1950). However, studies on Phaseolus vulgaris L. (Rebstock et al,, 1952) and on soybean (Freiberg & Clark, 1955) have shown that the increase in protein of stems is paralleled by an increased level of proteolytic enzymes and similarly that, in leaves, the concentrations of protein and digestive enzyme decrease together.…”

Section: Effects On Protein and Nucleic Acid Metabolismmentioning

confidence: 99%

The Mode of Action of Foliage‐applied Translocated Herbicides With Particular Reference to the Phenoxy‐acid Compounds

Robertson

Kirkwood

1969

Weed Research

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Effect of 2,4-Dichlorophenoxyacetic Acid on Proteolytic Activity of Red Kidney Bean Plants

Cited by 8 publications

References 1 publication

Changes in Nitrogen Fractions and Proteolytic Enzymes of Soybean Plants Treated with 2,4-Dichlorophenoxyacetic Acid.

Changes in Nitrogen Fractions and Proteolytic Enzymes of Soybean Plants Treated with 2,4-Dichlorophenoxyacetic Acid.

Studies in Plant Metabolism. VI. Effect of 2,4-D on the Metabolism of Aspartic Acid and Glutamic Acid in the Bean Plant.

The Mode of Action of Foliage‐applied Translocated Herbicides With Particular Reference to the Phenoxy‐acid Compounds

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