Effect of 2,4-dichlorophenoxyacetic acid on callus induction and plant regeneration in anther culture of wheat ( Triticum aestivum L.)

Zheng, M. Y.; Konzak, C. F.

doi:10.1007/s002990050712

Cited by 67 publications

(42 citation statements)

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“…All of the leaves were removed by severing leaf blades at their bases. The morphological features of tillers containing microspores at the mid-to late-uninucleate stage can easily be established for each genotype via microscopic examination of microspores in acetocarmine stain or distilled water (Zheng and Konzak 1999).…”

Section: Collection Of Tillersmentioning

confidence: 99%

“…Historically, haploids/doubled haploids have been predominantly produced by anther cultures (Chuang et al 1978;Guha and Maheshwari 1964;Hu and Yang 1986;Zheng and Konzak 1999;Zhou et al 1991;Ziauddin et al 1990). Only in recent years has substantial progress been made in wheat doubled haploid production through the culturing of isolated microspores (Gustafson et al 1995;Hu et al l995;Hu and Kasha 1997;Mejza et al 1993;Touraev et al 1996b;Tuvesson and Öhlund 1993).…”

Section: Introductionmentioning

confidence: 99%

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Culture of freshly isolated wheat (Triticum aestivum L.) microspores treated with inducer chemicals

Zheng¹,

Liu²,

Weng³

et al. 2001

Plant Cell Rep

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Microspores were isolated from wheat (Triticum aestivum L.) spikes by means of a micro-blender immediately following their removal from the donor plants. Isolated microspores were then subjected to a chemical treatment consisting of 0.18 mM 2-hydroxynicotinic acid, minerals and 9% maltose in the dark at 25°C for 38-52 h. Following purification via filtration and gradient centrifugation on 21% maltose, the microspores were cultured in the presence of excised ovaries in liquid medium at 27°C. Embryoid yield, percentage of green plants, and the frequency of spontaneously doubled haploids ranged from 360 to 4,914 embryoids per spike, 15% to 95%, and 39% to 78%, respectively. Other compounds that were effective in maintaining viability and triggering microspore embryogenesis were benzotriazole-5-carboxylic acid and violuric acid monohydrate. This system has proven to be highly efficient for producing doubled haploids over a range of genotypes.

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Section: Collection Of Tillersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Culture of freshly isolated wheat (Triticum aestivum L.) microspores treated with inducer chemicals

Zheng¹,

Liu²,

Weng³

et al. 2001

Plant Cell Rep

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“…2,4-D has been employed in most anther/microspore culture systems either as an auxin (to elicit rapid cell proliferation and callus formation) or as a co-trigger for microspore differentiation in combination with other pre-treatments (Xie et al 1995;Hoekstra et al 1996;Zheng & Konzak 1999;Rodrigues et al 2004;Shariatpanahi et al 2006b). …”

mentioning

confidence: 99%

“…The incubation of wheat anthers with 2.0 or 4.0 mg/l 2,4-D in an induction medium for 10 or 15 days was reported to be sufficient for the initiation of callus development (Zheng & Konzak 1999). However, the reduction or removal of 2,4-D in the medium beyond the initiation phase was essential for plant regeneration from calli.…”

mentioning

confidence: 99%

“…It is known that the first cell division during callus induction from anthers occurs within 2 days after inoculation for which 2,4-D plays a key role (Reynolds & Kitto 1992). However, for the transition from callus and/or embryo development to plant regeneration, the reduction or removal of 2,4-D is needed since new gene products are required (Zheng & Konzak 1999). The same effect of 2,4-D as an auxin was reported in rice isolated microspore culture (Xie et al 1995) in which 2,4-D was needed for induction of microspore-derived calli.…”

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confidence: 99%

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Effect of 2,4-D as a novel inducer of embryogenesis in microspores of Brassica napus L.

Ardebili¹,

Shariatpanahi²,

Amiri³

et al. 2011

CZECH J GENET PLANT

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Abstract:The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) applied at high concentrations for a short time was investigated as a novel stress for induction of microspore embryogenesis for the first time. Brassica napus L. cvs. Topas and Hyola 420 were used as model plants for testing this hypothesis. Microspores were subjected to 2,4-D at 4 concentrations (15, 25, 35 and 45 mg/l) for 15-45 min while the classical heat shock was used as the control treatment. Among 2,4-D treatments in Topas, the highest yield of torpedo-stage embryos was achieved at 15 mg/l 2,4-D for 30 min while more normal plantlets were produced when 2,4-D (25 mg/l for 30 min) was applied to the microspores. In Hyola 420 the results showed a lower number of embryos and normal plantlets at all concentrations of 2,4-D. Although Hyola 420 was almost equally embryogenic as Topas after heat shock treatment, large differences between genotypes (concerning embryogenic response) occurred after 2,4-D treatment. However, the mean number of embryos and regenerants was higher in heat shock as compared to 2,4-D induced stress (one magnitude of order). According to the results obtained, 2,4-D can be introduced as a new stress for induction of embryogenesis in microspores similarly like in zygotic and somatic cells. This novel stress is very important for plant species whose microspores are extremely sensitive to classical stresses.

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Development of a new wheat germplasm with high anther culture ability by using a combination of gamma-ray irradiation and anther culture

Zhao

Liu

Wang

et al. 2014

J. Sci. Food Agric.

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Effect of 2,4-dichlorophenoxyacetic acid on callus induction and plant regeneration in anther culture of wheat ( Triticum aestivum L.)

Cited by 67 publications

References 0 publications

Culture of freshly isolated wheat (Triticum aestivum L.) microspores treated with inducer chemicals

Culture of freshly isolated wheat (Triticum aestivum L.) microspores treated with inducer chemicals

Effect of 2,4-D as a novel inducer of embryogenesis in microspores of Brassica napus L.

Development of a new wheat germplasm with high anther culture ability by using a combination of gamma-ray irradiation and anther culture

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