Effect of 2,4-D, hydric stress and light on&lt;i&gt; indica&lt;/i&gt; rice (&lt;i&gt;Oryza sativa&lt;/i&gt;) somatic embryogenesis

Meneses, Allan; Flores, Dora; Muñoz, Miguel Luis López-Guadalupe; Arrieta, Griselda; Espinoza, Ana M.

doi:10.15517/rbt.v53i3-4.14598

Cited by 27 publications

(27 citation statements)

References 13 publications

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“…Gelrite was found to enhance callus induction and in vitro regeneration in Swarna (89.9%) and Mahsuri (93.4%) rice varieties [52]. Although use of agar as gelling agent to promote in vitro regeneration of rice has been reported [59][60][61]57], but Saharan et al [62] and Mensens et al [63] have reported higher regeneration frequency in indica rice varieties on media solidified with gelrite. Lin and Zhang [56] also have reported that 0.3% phytagel give higher callus induction.…”

Section: Resultsmentioning

confidence: 99%

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

Sah¹,

Kaur²

2014

J Rice Res

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A new and rapid protocol for production of embryogenic callus and plant regeneration has been reported in Oryza sativa cv. Kitaake. Callus cultures were established from seeds of Kitaake on MS medium supplemented with 2,4-D (3.0 mg/l), BAP (0.25 mg/l) and proline (0.6 g/l) gelled with phytagel (3.0 g/l), after 9 days of seed culturing. The embryogenic calli were regenerated on regeneration medium, i.e. MS medium supplemented with BAP (3.0 mg/l) and NAA (0.2 mg/l) and gelled with agar (8.0 g/l) in combination with phytagel (2.0 g/l) and regeneration occurred in 18 days. The regeneration percentage achieved was very high i.e 82.66% on the above medium. Rooting was achieved on half strength MS medium. The plantlets were hardened and transferred to soil in earthen pots. The regeneration protocol so developed through callus formation was highly reproducible. The plants showed normal growth and flowering under glass house conditions as well as in field conditions.

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Section: Resultsmentioning

confidence: 99%

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

Sah¹,

Kaur²

2014

J Rice Res

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“…Varietas rekalsitran dikenal karena rendahnya kemampuan tanaman dalam produksi dan regenerasi kalus (Khatun et al, 2003). Kultur jaringan melalui embriogenesis somatik menggunakan eksplan kalus merupakan salah satu teknik yang sering digunakan dan merupakan langkah kunci dalam bioteknologi padi (Meneses et al, 2005). Embriogenesis somatik adalah proses perkembangan sel somatik membentuk sel embriogenik melalui tahap-tahap perubahan morfologi tanpa proses peleburan sel gamet (Quiroz-Figuera et al, 2006).…”

Section: Pendahuluanunclassified

Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang)

Azizah¹,

Restanto²,

Sugiharto³

2017

J. I. Dasar

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Indica rice variety Ciherang is the most planted variety in Indonesia, but the micropropagation technique is restricted because it is known has low regeneration frequency and included as recalcitrant cultivar for tissue culture and tranformation activities. One of solution to resolve that problem is developing a technique of somatic embryogenesis in callus of ciherang rice cultivar. The aims of study were to determine medium composition for inducting embryogenic callus in Ciherang rice and to know the effectivity of rice regeneration using callus as explant. The methods were included induction of embryogenic callus in callus induction media (CIM) containing MS basal, Proline 600 mg/l, Casein Hidrolisat 300 mg/l, phytagel 2,5 g/l, BAP 0.25 mg/l, sukrosa 30 %, and 2,4-D in different concentration, from 2,4-D 0 mg/l as control (CIM 1), 2,4-D 2 mg/l (CIM 2), 2,4-D 3 mg/l (CIM 3), and 2,4-D 4 mg/l (CIM 4). It then be continued to regenerate the calli in RM 1 medium containing MS basal, NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 10 g/l, and sukrosa 30 %, pH 5,8 for six days in dark and RM2 medium containing MS basal , NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 8 g/l, sukrosa 30 %, pH 5,8 in light room. Results showed CIM 4 medium using 2,4-D 4 mg/l gave optimum result in calli induction with procentage 57,63% and CIM 3 using 2,4-D 3 mg/l gave optimum result in embryonic calli induction with procentage 53,63%. Result of embryonic calli gave regeneration frequency procentage is 9,6%. The total planlet obtained after five weeks old in regeneration medum were 11 planlets ready for aclimatization.

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“…Somatic embryogenesis, a regeneration technique effective in plant mass propagation, has been established in many species, e.g., Daucus carota (Lee et al, 2001;Nishiwaki et al, 2000), Oryza sativa L. (Meneses et al, 2005;Raval and Chattoo, 1993), Arabidopsis thaliana (Ikeda-Iwai et al, 2002;Luo and Koop, 1997), Phalaenopsis (Chen and Chang, 2006;Chung et al, 2006), and Cymbidium (Huan et al, 2004). The developmental process of somatic embryogenesis shares considerable similarity with that of zygotic embryogenesis (Zimmerman, 1993) because of the proposed conservation of molecular mechanisms underlying both processes (Higashi et al, 1998).…”

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Initiation and Cytological Aspects of Somatic Embryogenesis in Dendrobium candidum Wall ex Lindl.

Cui

Zhao

et al. 2017

horts

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To find the characteristics of somatic embryogenesis of orchids and elucidate the mechanism, we had previously established an efficient plant regeneration system via somatic embryogenesis in Dendrobium candidum Wall ex Lindl. In this study, a detailed cytological investigation was carried out on the initiation and developmental process of somatic embryogenesis. Based on our observations, the somatic embryogenesis in D. candidum originated from the transition of an embryonic callus cell to the initial somatic embryo cell, and the somatic embryos initiated from those cells. During the transition process, condensation and devacuolation successively occurred in the cytoplasm of the embryonic callus cells, giving rise to the formation of a typical initial somatic embryo cell with dense cytoplasm and a clear nucleus. One of the two pathways in somatic embryogenesis is the single-cell-derived somatic embryo which is generated from an inner initial somatic embryo cell in embryonic callus and develops into a globular somatic embryo in a way similar to zygotic embryogenesis and then keeps developing into a protocorm-like body (PLB). The other is a multiple-cell-derived somatic embryo which is generated from peripheral grouped initial somatic cells in embryonic calli and directly forms globular embryo or multicellular somatic proembryo, lacking the typical early stages of embryogenesis. Both pathways were observed in the somatic embryogenesis system, indicating that the culture system in D. candidum can be a useful tool for investigating the mechanisms underlying orchid embryogenesis.

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Effect of 2,4-D, hydric stress and light on<i> indica</i> rice (<i>Oryza sativa</i>) somatic embryogenesis

Cited by 27 publications

References 13 publications

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang)

Initiation and Cytological Aspects of Somatic Embryogenesis in Dendrobium candidum Wall ex Lindl.

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