Effect of 17% EDTA and MTAD on intracanal smear layer removal: A scanning electron microscopic study

Abstract

The purpose of this study was to determine the effectiveness of MTAD as the final irrigant to remove the smear layer, compared with that of 17% EDTA, both following root canal irrigation with 5.25% sodium hypochlorite (NaOCl). Fifty-five extracted maxillary and mandibular single-rooted human teeth were prepared by a crown-down technique using rotary 0.04 and 0.06 taper nickel-titanium files. 5.25% sodium hypochlorite was used as the intracanal irrigant. The canals were then treated with 5 mL of one of the following solutions as final rinse: 5.25% sodium hypochlorite, 17% EDTA or MTAD. The presence or absence of smear layer in the coronal, middle and apical portion of each canal was examined with a scanning electron microscope. The results showed that MTAD is an effective final rinse solution for removing the smear layer in canals irrigated with sodium hypochlorite. When 17% EDTA was used as a final rinse, the smear layer was removed from the middle and coronal thirds of canal preparations, but it was less effective in the apical third of the canals.

An in vitro scanning electron microscopic study comparing MTAD (intracanal irrigant) and various root biomodifiers on periodontally involved human teeth

“…MTAD had higher statistically significant number of patent and % patency of dentinal tubules when compared with TTC HCl (P = 0.0001), CA (P < 0.0001) and NS (P < 0.0001).This was in agreement with the study done by Torabinejad et al (2003), and Mozayeni et al (2009) who inferred that MTAD was more efficacious in removing smear layer and opening dentinal tubules of instrumented root canals because of being a combination agent of doxycycline, CA and a detergent tween-80, which reduces the surface tension and helps in better penetration of these acids into smear layer. Zhang et al (2003) demonstrated in his study that MTAD is less cytotoxic to the surrounding cells and tissues than EDTA.…”

“…The lowest score was achieved in Group I/MTAD, which indicates complete smear layer removal and complete opening of dentinal tubules, which was in accordance with the study by Mozayeni et al (2009) who stated that MTAD revealed the presence of more abundant and larger dentinal tubule opening when compared with other agents. [19] The result of group I was statistically significant when compared with group II, III, IV. [5,9,11] However until date, no in vitro or in vivo studies have been conducted utilizing MTAD as a root biomodifier and comparing these four agents.…”

Time-dependent effects of chitosan on dentin structures

“…Previous studies have shown that solutions with considerably higher concentrations than 0.2% chitosan and with longer application times than the 3 min used in this study (18,19) are necessary for removing this layer. Spanó et al (4) evaluated the cleaning capacity of 15% EDTA, 10% citric acid, 10% sodium citrate, apple vinegar, 5% acetic acid and 5% malic acid.…”

Effect of Ethylenediaminetetraacetic Acid on Root Fracture with Respect to Concentration at Different Time Exposures

“…However, NaOCl cannot dissolve inorganic components of dentin and thus prevent the formation of a smear layer during instrumentation (14). Therefore, demineralizing agents such as ethylenediaminetetraacetic acid (EDTA) have been recommended as adjuvants in root canal therapy (15).…”