Effect of 15% Alcohol Dependence on Alveolar Bone Loss and TNF-α Secretion in Wistar Rats

Abstract

The aim of the present study was to evaluate the effect of 15% alcohol dependence on ligature-induced alveolar bone loss and TNF-α secretion in Wistar rats. Thirty-three male Wistar rats aged 45-60 days (mean weight=253 g) were randomly allocated test or control groups. Test group (n=18) received 15% alcohol as liquid intake and control group (n=15) received water during the experimental period. TNF-α was analyzed by ELISA assay in 11 animals per group. After 14 days of alcohol/water intake, alcohol dependency was assessed and silk ligatures were placed around the left second upper molars. Ligature presence and body weight were checked weekly. After 40 days, animals were sacrificed and the maxillae were defleshed for morphometric analysis using standardized images. All animals in the test group displayed signs of alcohol dependency at day 14. No statistically significant differences in final body weight (334.83±21.38 vs. 322.48±30.65 g, p=0.20) were observed between groups. In relation to alveolar bone loss, no statistically significant difference was observed among test and control groups both for ligated teeth (0.76±0.06 vs. 0.74±0.10 mm, p=0.60) and unligated teeth (0.41±0.16 vs. 0.35±0.05 mm, p=0.22). The TNF-α secretion also did not display statistically significant differences between test and control groups (10.78±1.84 vs. 12.13±2.11 pg/mL, p=0.12). It may be concluded that 15% alcohol dependency was not capable to alter alveolar bone loss and TNF-α secretion in Wistar rats.

Ethanol binge drinking exposure affects alveolar bone quality and aggravates bone loss in experimentally-induced periodontitis

“…Different protocols have tested the effect of ethanol exposure on periodontal diseases in animal models with distinct results [ 6 , 7 , 10 , 13 , 22 , 23 ]. The idea behind such studies comes from the demonstrated association between alcohol consumption, especially in high doses, with periodontal diseases in populational studies even in a longitudinal approach [ 6 ].…”

“…Ethanol harmful effects in the oral cavity seem to be proportional to the alcohol-exposure pattern [ 7 , 12 , 13 ]. Enzymatic induction, immune modifier, as well as cementum formation and osteoblast activity reduction have been suggested to alcohol-related oral cavity health disorders [ 8 , 10 ].…”

Chronic Ethanol Consumption Induces Osteopenia via Activation of Osteoblast Necroptosis

“…However, the specific regulatory mechanism of TNF- α on bone cells and the molecular mechanism in the pathogenesis of osteoporosis remain unclear. It has been reported that TNF- α plays a role in promoting alcohol-induced osteoporosis [ 69 , 70 ]. Given that necroptosis is a special type of necrosis mainly mediated by the stimulating death receptor of TNF- α [ 27 , 71 ], we examined the level of TNF- α in mouse serum and osteoblastic culture medium after EtOH intervention and Nec-1 treatment by ELISA.…”

Efeito do consumo de álcool no desenvolvimento de lesão periapical induzida em ratos: uma análise microtomográfica

“…13 Different concentrations of alcoholic solutions have been used in several studies to simulate the alcoholic beverages consumed by humans. Wagner et al (2016) 14 used different concentrations of alcohol, defining low concentration as 5%, intermediate concentration as 13%, and high concentration as 20%. Another study also used 20% of alcohol as chronic consumption dose.…”