Effect of 1,25(OH&lt;sub&gt;2&lt;/sub&gt;D&lt;sub&gt;3&lt;/sub&gt; on the proliferation of human mesangial cells and their expression of Ki67

Zhao, Dan; Zhang, C.-J.; Yang, Run; Chen, J.-P.; Ma, Lan; Liu, G.; Yang, Xiao

doi:10.4238/gmr16029191

Cited by 6 publications

(5 citation statements)

References 10 publications

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“…Ki67 protein is a widely used cellular marker of proliferation present during all active phases of the cell cycle ( Zhao et al, 2017 ). We examined the expression of ki67 in tumors via immunohistochemistry ( Figure 4G ).…”

Section: Resultsmentioning

confidence: 99%

“…Following 28 days of culturing, ZnS significantly inhibited tumor growth, while TCONS-00026762 caused significantly tumor formation (Figures 4A-F). Ki67 protein is a widely used cellular marker of proliferation present during all active phases of the cell cycle (Zhao et al, 2017). We examined the expression of ki67 in tumors via immunohistochemistry (Figure 4G).…”

Section: Tcons-00026762 Regulated Smmc-7721-derived Tumor Progressionmentioning

confidence: 99%

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Dioscorea Zingiberensis New Saponin Inhibits the Growth of Hepatocellular Carcinoma by Suppressing the Expression of Long Non-coding RNA TCONS-00026762

Liu

Zhou

et al. 2021

Front. Pharmacol.

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Background: The etiology and carcinogenesis of hepatocellular carcinoma (HCC) are associated with various risk factors. Saponins extracted from Dioscorea zingiberensis C. H. Wright exhibit antitumor activity against HCC. This study aimed to investigate the effect and the underlying mechanism of Dioscorea Zingiberensis new saponin (ZnS) on HCC.Methods: Human HCC cell lines, Huh7 and SMMC-7721, were treated with different concentrations of ZnS. Cell apoptosis was determined via flow cytometry assay. Differentially expressed lncRNAs (DElncRNAs) in ZnS-treated SMMC-7721 cells were determined through RNA-sequence. The role of lncRNA TCONS-00026762 in HCC was investigated gain of function analysis, along with cell proliferation, apoptosis, and invasion in HCC cells. A subcutaneous xenograft of SMMC-7721 cell lines was established to study the effects of TCONS-00026762 in vivo. The expression of apoptosis-related proteins was detected in vivo and in vitro via western blotting.Results: ZnS inhibited the proliferation of HCC cell in a dose-dependent manner. ZnS could induce apoptosis in HCC cells. Illumina sequencing results showed that 493 DElncRNAs were identified in ZnS-treated SMMC-7721 cells. TCONS-00026762 expression was down-regulated in the ZnS-treated SMMC-7721 cells. TCONS-00026762 inhibited the effect of ZnS on the proliferation, apoptosis, and invasion of HCC cells. ZnS inhibited the tumor growth, while, TCONS-00026762 promoted tumor growth in vivo. Furthermore, ZnS and TCONS-00026762 regulated cell apoptotic pathways.Conclusion: ZnS significantly inhibits the viability, apoptosis, invasion, and tumorigenicity of HCC cells by regulating the expression of TCONS-00026,762. Our findings provide novel insights into the potential role of lncRNA in HCC therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Tcons-00026762 Regulated Smmc-7721-derived Tumor Progressionmentioning

confidence: 99%

Dioscorea Zingiberensis New Saponin Inhibits the Growth of Hepatocellular Carcinoma by Suppressing the Expression of Long Non-coding RNA TCONS-00026762

Liu

Zhou

et al. 2021

Front. Pharmacol.

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“…This was evidenced by an increase in Ki67-positive cells, which are a reliable marker of cell proliferation (Wang al., 2019a). Zhao et al (2017) showed that Ki67 protein and mRNA levels increased in response to EGF stimulation. The expression of cell cycle-related factor PCNA, an auxiliary protein of DNA polymerases δ and ε, was enhanced by 200 µg kg -1 EGF (Cheung et al, 2009).…”

Section: Discussionmentioning

confidence: 95%

“…Its homeostasis is required for gastrointestinal structural integrity and functional maturation (Grosse et al, 2011). Early weaning removes piglets from sow milk which contains a number of growth factors that play key roles in epithelial cell regeneration and renewal, and places them on a less digestible, solid diet (Zhao et al, 2017;Yang et al, 2016c). This transition-related loss of milk-borne growth factors is often accompanied by intestinal epithelial growth abnormalities, villus atrophy with its associated morbidity, gut barrier impairment, and inflammatory bowel diseases (Smith et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Epidermal growth factor improves intestinal morphology by stimulating proliferation and differentiation of enterocytes and mTOR signaling pathway in weaning piglets

Wang

Zhu

Yang

et al. 2019

Sci. China Life Sci.

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Epidermal growth factor (EGF) has been shown to improve piglet intestinal morphology and epithelial recovery. In an attempt to further understand the mechanisms behind these improvements, this study tested the hypothesis that dietary EGF may affect intestinal morphology by stimulating the proliferation and differentiation of enterocytes in weaning piglets. In piglets receiving 200 µg kg -1 EGF, crypt depth and villus height increased (P<0.05). Adding 400 µg kg -1 EGF increased villus height-to-crypt depth ratio (P<0.05), but reduced crypt depth (P<0.05). Dietary supplementation with 200 µg kg -1 EGF significantly increased the number of Ki67-positive cells (P<0.01) and tended to increase the mRNA level of proliferating cell nuclear antigen (P<0.10). However, this supplementation decreased the expression level of intestinal fatty acid-binding protein (P<0.05). Piglets fed with 400 µg kg -1 EGF had an increased mRNA level of intestinal alkaline phosphatase (P<0.05). The phosphorylation of mTOR (mammalian target of rapamycin) was observed in the 200 µg kg -1 EGF group. These results suggest that dietary supplementation with a low level of EGF improved piglet intestinal morphology through stimulating the proliferation and differentiation of enterocytes, and the mTOR signaling pathway may partly be involved in this process.

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“…The dose of DAPT requires strict control, however, a single low dose may not effectively prevent cancer progression. 1,25(OH) 2 D 3 has several biological activities, including maintaining a stable calcium environment, regulating immune function and affecting tumor biological activity, which may affect cell proliferation and apoptosis (32). 1,25(OH) 2 D 3 functions mainly through binding to a specific vitamin D receptor to inhibit myeloid leukemia cell proliferation (33).…”

Section: Discussionmentioning

confidence: 99%

Combined antitumor effects of 1,25‑dihydroxy vitamin�D3 and Notch inhibitor in liver cancer

et al. 2018

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The combined antitumor effects of 1,25‑dihydroxy vitamin D3 [1,25(OH)2D3] and the Notch inhibitor N‑[N‑-(3,5‑difluorophenacetyl)‑L‑alanyl]‑S‑phenylglycine t‑butyl ester (DAPT, a synthetic γ secretase inhibitor) in liver cancer cells remain to be fully elucidated. In the present study, HepG2 cells were divided into six groups and different treatments were applied: Control, 10‑10 M 1,25(OH)2D3, 10‑8 M 1,25(OH)2D3, 10‑6 M 1,25(OH)2D3, 1 µM DAPT, 5 µM DAPT, 10 µM DAPT, and 10‑6 M 1,25(OH)2D3 + 10 µM DAPT. The proliferation, cell cycle, apoptosis, migration and invasion of the cells were then examined. The expression levels of Notch and its ligand Jagged were detected by reverse transcription‑quantitative polymerase chain reaction and western blot analyses. The results revealed that 1,25(OH)2D3 inhibited cell proliferation, migration and invasion; arrested cell cycle at the G1 phase, and promoted apoptosis in a concentration‑dependent manner between 10‑10 and 10‑6 M. DAPT inhibited cell proliferation, migration and invasion, arrested cell cycle at the G1 phase, and promoted apoptosis in a concentration‑dependent manner between 1 and 10 µM. Additionally, 1,25(OH)2D3 and/or DAPT reduced the expression of Notch1, Notch2, Jagged1 and Jagged2. The co‑application of 10 µM DAPT further increased the anticancer effect of 10‑6 M 1,25(OH)2D3. Collectively, these results indicated that the treatment of HepG2 cells with 1,25(OH)2D3 inactivated Notch signaling, prevented proliferation, migration and invasion, and promoted apoptosis. The combined application of 1,25(OH)2D3 with DAPT may be a useful treatment for preventing the onset or progression of liver cancer.

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Effect of 1,25(OH<sub>2</sub>D<sub>3</sub> on the proliferation of human mesangial cells and their expression of Ki67

Cited by 6 publications

References 10 publications

Dioscorea Zingiberensis New Saponin Inhibits the Growth of Hepatocellular Carcinoma by Suppressing the Expression of Long Non-coding RNA TCONS-00026762

Dioscorea Zingiberensis New Saponin Inhibits the Growth of Hepatocellular Carcinoma by Suppressing the Expression of Long Non-coding RNA TCONS-00026762

Epidermal growth factor improves intestinal morphology by stimulating proliferation and differentiation of enterocytes and mTOR signaling pathway in weaning piglets

Combined antitumor effects of 1,25‑dihydroxy vitamin�D3 and Notch inhibitor in liver cancer

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