Effect of 1.10-phenanthroline, a photodynamic herbicide on the development and structure of maize chloroplasts

Mostowska, Agnieszka

doi:10.1007/s11738-998-0029-x

Cited by 5 publications

(1 citation statement)

References 22 publications

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“…Cell expansion is driven by turgor pressure, and in healthy tissues is usually limited by the extensibility of the cell wall or sometimes by the wall yield threshold (Cosgrove, 1993; Kutschera, 2001). Theoretically, therefore, a herbicide could inhibit cell expansion by decreasing the turgor pressure (Mostowska, 1998). In the present paper we have tested for possible effects of OAC on processes that govern turgor and wall acidification.…”

Section: Introductionmentioning

confidence: 99%

The novel herbicide oxaziclomefone inhibits cell expansion in maize cell cultures without affecting turgor pressure or wall acidification

O'Looney

Fry

2005

New Phytologist

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Summary• Oxaziclomefone [OAC; IUPAC name 3-(1-(3,5-dichlorophenyl)-1-methylethyl)-3,4-dihydro-6-methyl-5-phenyl-2 H -1,3-oxazin-4-one] is a new herbicide that inhibits cell expansion in grass roots. Its effects on cell cultures and mode of action were unknown. In principle, cell expansion could be inhibited by a decrease in either turgor pressure or wall extensibility.• Cell expansion was estimated as settled cell volume; cell division was estimated by cell counting. Membrane permeability to water was measured by a novel method involving simultaneous assay of the efflux of 3 H 2 O and [ 14 C]mannitol from a 'bed' of cultured cells. Osmotic potential was measured by depression of freezing point.• OAC inhibited cell expansion in cultures of maize ( Zea mays ), spinach ( Spinacia oleracea ) and rose ( Rosa sp.), with an ID 50 of 5, 30 and 250 n M , respectively. In maize cultures, OAC did not affect cell division for the first 40 h. It did not affect the osmotic potential of cell sap or culture medium, nor did it impede water transport across cell membranes. It did not affect cells' ability to acidify the apoplast (medium), which may be necessary for 'acid growth'.• As OAC did not diminish turgor pressure, its ability to inhibit cell expansion must depend on changes in wall extensibility. It could be a valuable tool for studies on cell expansion.

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Section: Introductionmentioning

confidence: 99%

The novel herbicide oxaziclomefone inhibits cell expansion in maize cell cultures without affecting turgor pressure or wall acidification

O'Looney

Fry

2005

New Phytologist

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Aspects of programmed cell death during leaf senescence of mono- and dicotyledonous plants

et al. 2000

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Monitoring the mitochondrial transmembrane potential with the JC-1 fluorochrome in programmed cell death during mesophyll leaf senescence

Simeonova¹,

Garstka²,

Kozioł-Lipińska³

et al. 2004

Protoplasma

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Analysis of the mitochondrial transmembrane potential (Delta Psi(m)) with the help of the JC-1 fluorochrome (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide) during mesophyll leaf senescence was performed in order to determine whether a reduction of Delta Psi(m) takes place during mesophyll senescence and whether plant mitochondria, like mammalian ones, might be involved in the induction of programmed cell death. Fluorescence analysis of mesophyll protoplasts of Pisum sativum in a confocal microscope, fluorescent spectra analysis and time dependence of fluorescence intensity of monomers and of J-aggregates revealed that JC-1 is incorporated and accumulated specifically in plant mitochondria. Analysis of Delta Psi(m) during mesophyll protoplast senescence revealed that two subpopulations of mitochondria which differ in Delta Psi(m) exist in all analyzed stages of leaf senescence. The first subpopulation contains mitochondria with red fluorescence of J-aggregates due to an unperturbed high Delta Psi(m). The second subpopulation comprises mitochondria with green fluorescence of monomers due to a low Delta Psi(m), proving total depolarization of mitochondrial membranes. Fluorescence analysis demonstrated that even in the latest analyzed stages of leaf senescence, mitochondria with a high Delta Psi(m) still exist. Fluorometric measurements revealed that the fluorescence intensity of J-aggregates decreases with the age of plants, which indicates that a reduction of Delta Psi(m) during the mesophyll senescence process takes place; however, it does not take place within the whole population of mitochondria of the same protoplast. The reason of this can be due to a dramatic reorganization of mitochondria in mesophyll cells and the appearance of large mitochondria with local heterogeneity of Delta Psi(m) in the oldest analyzed stages. All mitochondria in every stage of senescence maintained their membrane organization even when their size, distribution, and spatial organization in protoplasts changed dramatically. We stated that the reduction of Delta Psi(m) does not directly induce programmed cell death in mesophyll cells, as opposed to animal apoptosis.

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Effect of 1.10-phenanthroline, a photodynamic herbicide on the development and structure of maize chloroplasts

Cited by 5 publications

References 22 publications

The novel herbicide oxaziclomefone inhibits cell expansion in maize cell cultures without affecting turgor pressure or wall acidification

The novel herbicide oxaziclomefone inhibits cell expansion in maize cell cultures without affecting turgor pressure or wall acidification

Aspects of programmed cell death during leaf senescence of mono- and dicotyledonous plants

Monitoring the mitochondrial transmembrane potential with the JC-1 fluorochrome in programmed cell death during mesophyll leaf senescence

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