Effect of 0-(β-Hydroxyethyl)-Rutoside on the Glucose Metabolism of Cultured Human Varicose Saphenous Veins

Abstract

The flavonoid O-(beta-hydroxyethyl)-rutoside appears to have a slight inhibitory action upon the oxidative metabolism and lactate production of culured human varicose saphenous veins. The possibility that exogenous glucose would be utilized in other metabolic pathways is discussed.

Venous Hypoxia: A Poorly Studied Etiological Factor of Varicose Veins

“…An early study of oxidative metabolism and glucose transport in varicose vein organ cultures suggested that the oxygen consumption of human varicose veins was relatively low and comparable to healthy veins investigated in other studies [55]. However, in the absence of a control group, it is difficult to draw firm conclusions regarding oxygen consumption between varicose and non-varicose veins from this study [55]. …”

“…Venoactive drugs were also shown to target complexes I and III of the mitochondrial respiratory chain or adenine nucleotide translocase, reduce oxidative stress, and increase ATP synthesis during hypoxia [8,41,64]. A further study used an ex vivo vein explant model and found that flavonoids significantly reduced the oxygen consumption of varicose veins [55]. …”

“…The most commonly studied group of pharmacological agents in this context has been the venoactive drugs such as flavonoids [19,41,55,57,62]. Venoactive drugs are often derived from plant extracts, and their therapeutic actions include increased venous tone, reduced vein wall inflammation and decreased capillary permeability [6,63].…”

Cell death pattern of a varicose vein organ culture model

“…1 Organ cultures have often been used to study the effects of various exogenous factors and stimuli including potential pathological stresses and therapeutic agents on the organ or tissue of interest including in diseases of venous tissues. [5][6][7][8][9][10][11] In the study of VV disease, organ cultures of varicosities have been used, and they were often relatively similar, with some modifications, to a non-varicose saphenous vein organ culture model that was initially developed for the study of neointimal hyperplasia in vein grafts. [3][4][5]7,12,13 Although the original nonvaricose saphenous vein organ culture model was well validated, 14,15 the viability of the VV organ culture model has never been reported.…”

“…5–11 In the study of VV disease, organ cultures of varicosities have been used, and they were often relatively similar, with some modifications, to a non-varicose saphenous vein organ culture model that was initially developed for the study of neointimal hyperplasia in vein grafts. 3–5,7,12,13 Although the original non-varicose saphenous vein organ culture model was well validated, 14,15 the viability of the VV organ culture model has never been reported. It is unwise to extrapolate results and assume that VV and non-VV organ culture have similar viability despite similarities in technical set-up of the organ culture since VVs are different structurally, physiologically and biochemically from non-VVs.…”