Effect liposome Nanoparticles Loaded with Natural Products Isolated From Commiphora myrrh on Plasminogen Activator Inhibitor-1 in Mice Induced Aortic Atherosclerosis

Abstract

HE study included the separation and diagnosis of various natural products from the Myrrh plant, where the oily substance and its fatty acids were separated, which were identified by applying capillary column gas chromatography (CGC) technology. Some flavonoids and alkaloids were also separated. The flavonoids were identified by applying high-performance liquid chromatography (HPLC) technology. The effect of these products on plasminogen activator inhibitor-1 (PAI-1) was studied and it was found that they had a positive effect in reducing the level of PAI-1 and some other measured variables. In addition, the study examined the effect of these natural products on some biochemical parameters related to aortic stiffness in the blood serum of mice induced with the disease. Biochemical variables included: low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC), triglycerides (TG), interleukin 6 (IL-6), and... Tumor necrosis alpha (TNF-alpha). The results showed that the concentrations of PAI-1, IL-6, LDL, TG, TC, and TNF-α increased significantly in infected mice, compared to healthy mice, and a significant decrease in the concentration of HDL was observed. Liposome nanoparticles (LNPs) were prepared from natural products of myrrh plant and characterized using field emission scanning electron microscopy (FE-SEM). Nanoparticles loaded with natural products showed a positive therapeutic effect in mice in which the disease was established through tissue sections of experimental animals.