Effect-directed analysis via hyphenated high-performance thin-layer chromatography for bioanalytical profiling of sunflower leaves

Móricz, Ágnes M.; Ott, Péter G.; Yüce, Imanuel; Darcsi, András; Béni, Szabolcs; Morlock, Gertrud E.

doi:10.1016/j.chroma.2017.12.034

Cited by 31 publications

(10 citation statements)

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“…Despite of being less intensively modernized in comparison to column-based techniques like (U)HPLC, this method is still favored when cost-effectiveness and simplicity of sample preparation is important, for example in herbal industries and educational use. Some recent developments in mass spectrometry hyphenation in form of matrix transfer or DART (direct analysis in real time) used already in studying of other species (Móricz et al, 2018 ) should be useful also for C. majus alkaloids.…”

Section: Methods For Analysis Of Active Components From Chementioning

confidence: 99%

Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

et al. 2018

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As antique as Dioscorides era are the first records on using Chelidonium as a remedy to several sicknesses. Inspired by the “signatura rerum” principle and an apparent ancient folk tradition, various indications were given, such as anti-jaundice and cholagogue, pain-relieving, and quite often mentioned—ophthalmological problems. Central and Eastern European folk medicine has always been using this herb extensively. In this region, the plant is known under many unique vernacular names, especially in Slavonic languages, associated or not with old Greek relation to “chelidon”—the swallow. Typically for Papaveroidae subfamily, yellow-colored latex is produced in abundance and leaks intensely upon injury. Major pharmacologically relevant components, most of which were first isolated over a century ago, are isoquinoline alkaloids—berberine, chelerythrine, chelidonine, coptisine, sanguinarine. Modern pharmacology took interest in this herb but it has not ended up in gaining an officially approved and evidence-based herbal medicine status. On the contrary, the number of relevant studies and publications tended to drop. Recently, some controversial reports and sometimes insufficiently proven studies appeared, suggesting anticancer properties. Anticancer potential was in line with anecdotical knowledge spread in East European countries, however, in the absence of directly-acting cytostatic compounds, some other mechanisms might be involved. Other properties that could boost the interest in this herb are antimicrobial and antiviral activities. Being a common synanthropic weed or ruderal plant, C. majus spreads in all temperate Eurasia and acclimates well to North America. Little is known about the natural variation of bioactive metabolites, including several aforementioned isoquinoline alkaloids. In this review, we put together older and recent literature data on phytochemistry, pharmacology, and clinical studies on C. majus aiming at a critical evaluation of state-of-the-art from the viewpoint of historical and folk indications. The controversies around this herb, the safety and drug quality issues and a prospective role in phytotherapy are discussed as well.

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Section: Methods For Analysis Of Active Components From Chementioning

confidence: 99%

Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

et al. 2018

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“…On-line coupling of UV detectors with Q-Orbitrap were also reported in characterization [55], metabolic [56] and dereplication [57] studies, demonstrating that (U)HPLC-DAD-Q-Orbitrap-MS hyphenation can effectively detect and quantify small organic compounds such as phenolics and sesquiterpene lactones. Móricz et al [58] reported the benefit of highperformance thin-layer chromatography (HPTLC)-hyphenations in the discovery and characterization of active natural compounds. Non-targeted screening of sunflower leaf extract by HPTLC-ESI-Q-Orbitrap-MS allowed the targeted characterization of components exhibiting antioxidant activity, and diterpenes with antibacterial and/or cholinesterase enzyme inhibitory effects.…”

Section: Orbitrap Ms Methodsmentioning

confidence: 99%

Recent applications of high resolution mass spectrometry for the characterization of plant natural products

Álvarez-Rivera

Ballesteros-Vivas

Parada-Alfonso

et al. 2019

TrAC Trends in Analytical Chemistry

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Recently, the high analytical power provided by the new HRMS instruments (working in the MS or MS/MS mode, stand alone or hyphenated with separation techniques) is making more feasible the characterization of plant secondary metabolites. In this regard, HRMS-based techniques are becoming the technique of choice, since they are capable of answering many questions regarding analytical characterization of secondary metabolites, usually found in very complex matrices, including their structure, composition and concentration in a very fast and sensitive way. In this manuscript we will provide a critical and updated revision of this topic covering the works published in the last 4 years (2015)(2016)(2017)(2018), including the different configurations in which HRMS may be used (stand alone or hyphenated), the data treatment, the strategies for the identification of unknown metabolites, the current software for structure elucidation, as well as the last trends and future outlooks in this hot area of research.

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“…Comparison of HPTLC-UV/Vis chromatograms before and after derivatization using the anisaldehyde-sulfuric acid reagent and HPTLC-Vis-EDA autograms allowed determining bioactive compound zones, which then were subjected to analysis by DART with a high-resolution mass analyzer. A similar approach was used for bioanalytical profiling of sunflower leaves (Móricz et al, 2018).…”

Section: Thin-layer Chromatography and Novel Desorption/ionization Mass-spectrometry Techniquesmentioning

confidence: 99%

Recent Advances in Combinations of TLC With MALDI and Other Desorption/Ionization Mass-Spectrometry Techniques

2021

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The combination of planar chromatography with desorption/ionization mass-spectrometry (MS) techniques provides chemists with unique tools for fast and simple separation of mixtures followed by the detection of analytes by the most powerful analytical method. Since its introduction in the early 1990s, thin-layer chromatography (TLC)/matrix-assisted mass spectrometry (MALDI) has been used for the analysis of a wide range of analytes, including natural and synthetic organic compounds. Nowadays, new desorption/ionization approaches have been developed and applied in conjunction with planar chromatography competing with MALDI. This review covers recent developments in the combination of TLC with various desorption/ionization MS methods which were made in recent several years.

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Effect-directed analysis via hyphenated high-performance thin-layer chromatography for bioanalytical profiling of sunflower leaves

Cited by 31 publications

References 50 publications

Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

Recent applications of high resolution mass spectrometry for the characterization of plant natural products

Recent Advances in Combinations of TLC With MALDI and Other Desorption/Ionization Mass-Spectrometry Techniques

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