Effect directed analysis and TLC screening of Schisandra chinensis fruits

Sobstyl, Ewelina; Szopa, Agnieszka; Ekiert, Halina; Gnat, Sebastian; Typek, Rafał; Choma, Irena

doi:10.1016/j.chroma.2020.460942

Cited by 33 publications

(30 citation statements)

References 31 publications

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“…The fruit extract is much more active against B. subtilis than the leaves ones ( Figure 2 b, Section 4.3.3 ). There is a large inhibition zone at the start, just as it was observed earlier in the case of the S. chinensis fruit where citric acid was detected [ 20 ].…”

Section: Resultssupporting

confidence: 58%

“…The enormously high content of lignans in fraction VII is probably related to the zones of AChE inhibition seen at R F 0.92–0.99, especially for fruits ( Section 2.2.4 and Figure 2 d). As it was proved, many dibenzocyclooctadiene lignans are responsible for AChE inhibition [ 20 ]. Hung et al [ 27 ] carried out an AChE activity assay using an acetylthiocholine iodide substrate, based on a colorimetric method previously described by Ellman [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…In the method, a TLC plate is immersed or sprayed with an enzyme solution and its substrate to obtain spots of enzyme inhibitors that differ in color from the background. The enzyme inhibitors may be the potential ingredients of drugs used to cure various diseases such as Alzheimer’s, Parkinson’s, depression, diabetes, obesity, and viral infections [ 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Schisandra rubriflora Fruit and Leaves as Promising New Materials of High Biological Potential: Lignan Profiling and Effect-Directed Analysis

et al. 2022

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The effect-directed detection (EDD) of Schisandra rubriflora fruit and leaves extracts was performed to assess their pharmacological properties. The EDD comprised TLC—direct bioautography against Bacillus subtilis, a DPPH assay, as well as α-glucosidase, lipase, tyrosinase, and acetylcholinesterase (AChE) inhibition assays. The leaf extracts showed stronger antioxidant activity than the fruit extract as well as inhibition of tyrosinase and lipase. The fruit extract was found to be extremely active against B. subtilis and to inhibit α-glucosidase and AChE slightly more than the leaf extracts. UHPLC–MS/MS analysis was carried out for the bioactive fractions and pointed to the possible anti-dementia properties of the dibenzocyclooctadiene lignans found in the upper TLC fractions. Gomisin N (518 mg/100 g DW), schisanhenol (454 mg/100 g DW), gomisin G (197 mg/100 g DW), schisandrin A (167 mg/100 g DW), and gomisin O (150 mg/100 g DW) were the quantitatively dominant compounds in the fruit extract. In total, twenty-one lignans were found in the bioactive fractions.

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Section: Resultssupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Schisandra rubriflora Fruit and Leaves as Promising New Materials of High Biological Potential: Lignan Profiling and Effect-Directed Analysis

et al. 2022

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“…In the present study, the dried leaf with a leaf blade and a stalk was extracted with acetone or methanol, a TLC-based simple identification method was performed, and the results showed that the acetone extract had specific spots visualized with UV lamp irradiation (254 and 365 nm) and phosphomolybdic acid staining. The results indicated that the crude extract of MB may contain aromatic compounds with a certain number of conjugated double bond ( 30 – 35 ) and/or an antioxidant compound with a hydroxy group ( 36 , 37 ), suggesting polyphenols and/or flavonoids as potential active components. The extract of Chinese propolis contains polyphenols and flavonoids ( 38 – 40 ).…”

Section: Discussionmentioning

confidence: 99%

Extracts ofMusa basjooinduce growth inhibition and changes in the protein expression of cell cycle control molecules in human colorectal cancer cell lines

et al. 2022

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Musa basjoo (MB) is a species of the banana plant belonging to the genus Musa that has been used as a folk medicine. However, evidence-based biological activities and the molecular mechanism of action of MB are unknown. Thus, the aim of the present study was to examine whether the crude dried leaf extracts of MB inhibit the growth of colorectal (HT29 and HCT116) and other types (HepG2, MCF-7 and PC-3) of human cancer cell lines. Crude extracts of MB inhibited the growth of cells with IC 50 values of 136 µg/ml (acetone extract, HT29), 51 µg/ml (acetone extract, HCT116), 45 µg/ml (acetone extract, HepG2), 40 µg/ml (acetone extract, MCF-7), 29 µg/ml (acetone extract, PC-3), 175 µg/ml (methanol extract, HT29), 137 µg/ml (methanol extract, HCT116), 102 µg/ml (methanol extract, HepG2), 85 µg/ml (methanol extract, MCF-7), and 85 µg/ml (methanol extract, PC-3) in colony formation assays, and 126 µg/ml (acetone extract, HT29), 68 µg/ml (acetone extract, HCT116), 260 µg/ml (methanol extract, HT29), and 216 µg/ml (methanol extract, HCT116) in MTT assays. Thin layer chromatography analysis revealed the potential existence of aromatic compounds in the acetone extract of MB. Flow cytometric analysis indicated that the percentage of cells in G1 increased, and this was associated with a concomitant decrease of cells in the S and/or G2-M phases of the cell cycle. When colorectal cancer cells were treated with acetone extract of MB, there was a marked decrease in the levels of expression of the cyclin D1, cyclin E, cdk2 and cdk4 proteins and a marked increase in the levels of the expression of the p21 CIP1 , p27 KIP1 , and p53 proteins, but those of apoptosis-associated protein PARP did not change. There was a tendency for acetone extract of MB to inhibit xenograft tumor growth in mice. Collectively, the crude extracts of MB contain active components that exert growth inhibition of human cancer cells. This is the first systematic study of the anticancer activity of MB and may broaden insights into the possible clinical approach of specific herbal medicines.

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“…Schisandra chinensis is a dry and mature fruit of perennial deciduous woody vines [ 12 ], and it has been used as a Chinese traditional herbal medicine [ 13 ]. Schisandra chinensis is rich in lignans, which have a variety of pharmacological effects and display good therapeutic effects on many diseases [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Fermentation Process of Pomegranate Peel and Schisandra Chinensis and the Biological Activities of Fermentation Broth: Antioxidant Activity and Protective Effect Against H2O2-induced Oxidative Damage in HaCaT Cells

Liu

Cheng

et al. 2021

Molecules

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In this study, the lactobacillus fermentation process of pomegranate (Punica granatum L.) peel and Schisandra chinensis (Turcz.) Baill (PP&SC) was optimized by using the response surface method (RSM) coupled with a Box-Behnken design. The optimum fermentation condition with the maximal yield of ellagic acid (99.49 ± 0.47 mg/g) was as follows: 1:1 (w:w) ratio of pomegranate peel to Schisandra chinensis, 1% (v:v) of strains with a 1:1 (v:v) ratio of Lactobacillus Plantarum to Streptococcus Thermophilus, a 37 °C fermentation temperature, 33 h of fermentation time, 1:20 (g:mL) of a solid–liquid ratio and 3 g/100 mL of a glucose dosage. Under these conditions, the achieved fermentation broth (FB) showed stronger free radical scavenging abilities than the water extract (WE) against the ABTS+, DPPH, OH− and O2− radicals. The cytotoxicity and the protective effect of FB on the intracellular ROS level in HaCaT cells were further detected by the Cell Counting Kit-8 (CCK-8) assay. The results showed that FB had no significant cytotoxicity toward HaCaT cells when its content was no more than 8 mg/mL. The FB with a concentration of 8 mg/mL had a good protective effect against oxidative damage, which can effectively reduce the ROS level to 125.94% ± 13.46% (p < 0.001) compared with 294.49% ± 11.54% of the control group in H2O2-damaged HaCaT cells. The outstanding antioxidant ability and protective effect against H2O2-induced oxidative damage in HaCaT cells promote the potential for the FB of PP&SC as a functional raw material of cosmetics.

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Effect directed analysis and TLC screening of Schisandra chinensis fruits

Cited by 33 publications

References 31 publications

Schisandra rubriflora Fruit and Leaves as Promising New Materials of High Biological Potential: Lignan Profiling and Effect-Directed Analysis

Schisandra rubriflora Fruit and Leaves as Promising New Materials of High Biological Potential: Lignan Profiling and Effect-Directed Analysis

Extracts ofMusa basjooinduce growth inhibition and changes in the protein expression of cell cycle control molecules in human colorectal cancer cell lines

Optimization of Fermentation Process of Pomegranate Peel and Schisandra Chinensis and the Biological Activities of Fermentation Broth: Antioxidant Activity and Protective Effect Against H2O2-induced Oxidative Damage in HaCaT Cells

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