Effect-based trigger values for in vitro bioassays: Reading across from existing water quality guideline values

Escher, Beate I.; Neale, Peta A.; Leusch, Frédéric D.L.

doi:10.1016/j.watres.2015.05.049

Cited by 86 publications

(87 citation statements)

References 60 publications

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“…Adaptive stress responses are key events (Simmons et al 2009) and very recent work has elucidated the key event relationships of the toxicologically relevant Nrf2and p53-mediated adaptive stress responses in response to reference chemicals (Hiemstra et al 2017, Wink et al 2014, Wink et al 2017. Nrf2, which activates the oxidative stress response, was most responsive in water samples in a large number of studies , Escher et al 2015b, Neale et al 2015, while p53 activity was only occasionally detected in water samples (e.g., Yeh et al 2014). A recent study using the Attagene battery as part of the ToxCast high-throughput screening assays identified this same transcription factor Nrf2 as being relevant for surface water quality monitoring and also identified the hypoxia pathway as relevant (Schroeder et al 2016).…”

Section: Assembling a Bioanalytical Test Battery For Surface Water Qumentioning

confidence: 99%

“…In turn this does not mean that for water quality monitoring that many different assays indicative of the one endpoint should be used or that one assay should be favored over others. However, when undertaking mixture toxicity modeling or when deriving effect-based trigger values, one should use data from the same bioassay as the BEQ value will be specific for each assay (Escher et al 2015a).…”

Section: Comparing Cellular Effects With Whole Organism Specific Effectsmentioning

confidence: 99%

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Development of a bioanalytical test battery for water quality monitoring: Fingerprinting identified micropollutants and their contribution to effects in surface water

et al. 2017

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Surface waters can contain a diverse range of organic pollutants, including pesticides, pharmaceuticals and industrial compounds. While bioassays have been used for water quality monitoring, there is limited knowledge regarding the effects of individual micropollutants and their relationship to the overall mixture effect in water samples. In this study, a battery of in vitro bioassays based on human and fish cell lines and whole organism assays using bacteria, algae, daphnids and fish embryos was assembled for use in water quality monitoring. The selection of bioassays was guided by the principles of adverse outcome pathways in order to cover relevant steps in toxicity pathways known to be triggered by environmental water samples. The effects of 34 water pollutants, which were selected based on hazard quotients, available environmental quality standards and mode of action information, were fingerprinted in the bioassay test battery. There was a relatively good agreement between the experimental results and available literature effect data. The majority of the chemicals were active in the assays indicative of apical effects, while fewer chemicals had a response in the specific reporter gene assays, but these effects were typically triggered at lower concentrations. The single chemical effect data were used to improve published mixture toxicity modeling of water samples from the Danube River. While there was a slight increase in the fraction of the bioanalytical equivalents explained for the Danube River samples, for some endpoints less than 1% of the observed effect could be explained by the studied chemicals. The new mixture models essentially confirmed previous findings from many studies monitoring water quality using both chemical analysis and bioanalytical tools. In short, our results indicate that many more chemicals contribute to the biological effect than those that are typically quantified by chemical monitoring programs or those regulated by environmental quality standards. This study not only demonstrates the utility of fingerprinting single chemicals for an improved understanding of the biological effect of pollutants, but also highlights the need to apply bioassays for water quality monitoring in order to prevent underestimation of the overall biological effect.

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Section: Assembling a Bioanalytical Test Battery For Surface Water Qumentioning

confidence: 99%

Section: Comparing Cellular Effects With Whole Organism Specific Effectsmentioning

confidence: 99%

Development of a bioanalytical test battery for water quality monitoring: Fingerprinting identified micropollutants and their contribution to effects in surface water

et al. 2017

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“…and varying composition, such as surface and waste water Napierska et al 2018). There is an ongoing discussion whether the setting of thresholds, such as environmental quality standards could or should be effect-based (Escher et al 2015(Escher et al , 2018. However, with the increasing number of effect-based methods, it is important to identify how these tests can be used to complement chemical monitoring without increasing the overall monitoring burden.…”

Section: Discussionmentioning

confidence: 99%

Regulatory assessment and risk management of chemical mixtures: challenges and ways forward

Bopp

Kienzler

Richarz

et al. 2019

Critical Reviews in Toxicology

160

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This paper summarizes current challenges, the potential use of novel scientific methodologies, and ways forward in the risk assessment and risk management of mixtures. Generally, methodologies to address mixtures have been agreed; however, there are still several data and methodological gaps to be addressed. New approach methodologies can support the filling of knowledge gaps on the toxicity and mode(s) of action of individual chemicals. (Bio)Monitoring, modeling, and better data sharing will support the derivation of more realistic co-exposure scenarios. As knowledge and data gaps often hamper an in-depth assessment of specific chemical mixtures, the option of taking account of possible mixture effects in single substance risk assessments is briefly discussed. To allow risk managers to take informed decisions, transparent documentation of assumptions and related uncertainties is recommended indicating the potential impact on the assessment. Considering the large number of possible combinations of chemicals in mixtures, prioritization is needed, so that actions first address mixtures of highest concern and chemicals that drive the mixture risk. As chemicals with different applications and regulated separately might lead to similar toxicological effects, it is important to consider chemical mixtures across legislative sectors. ARTICLE HISTORY

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“…Until we can quantitatively extrapolate from in vitro to in vivo (steps 1 and 2 above) and quantify the repair ability for each type of dysfunction, it will be difficult to accurately link an in vitro response to an in vivo adverse effect. In the meantime, several different approaches have been proposed to produce effects-based trigger (EBT) values, including reading across from current chemical guidelines (Escher et al 2015 or de novo derivation (Brand et al 2013;Jarošová et al 2014) and how to use them in a practical context (Leusch and Snyder 2015;Ron et al 2017).…”

Section: Tools For Improving Risk Assessmentmentioning

confidence: 99%

Towards Sustainable Environmental Quality: Priority Research Questions for the Australasian Region of Oceania

Gaw

Harford

Pettigrove

et al. 2019

Integr Envir Assess & Manag

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Environmental challenges persist across the world, including the Australasian region of Oceania, where biodiversity hotspots and unique ecosystems such as the Great Barrier Reef are common. These systems are routinely affected by multiple stressors from anthropogenic activities, and increasingly influenced by global megatrends (e.g., the food–energy–water nexus, demographic transitions to cities) and climate change. Here we report priority research questions from the Global Horizon Scanning Project, which aimed to identify, prioritize, and advance environmental quality research needs from an Australasian perspective, within a global context. We employed a transparent and inclusive process of soliciting key questions from Australasian members of the Society of Environmental Toxicology and Chemistry. Following submission of 78 questions, 20 priority research questions were identified during an expert workshop in Nelson, New Zealand. These research questions covered a range of issues of global relevance, including research needed to more closely integrate ecotoxicology and ecology for the protection of ecosystems, increase flexibility for prioritizing chemical substances currently in commerce, understand the impacts of complex mixtures and multiple stressors, and define environmental quality and ecosystem integrity of temporary waters. Some questions have specific relevance to Australasia, particularly the uncertainties associated with using toxicity data from exotic species to protect unique indigenous species. Several related priority questions deal with the theme of how widely international ecotoxicological data and databases can be applied to regional ecosystems. Other timely questions, which focus on improving predictive chemistry and toxicology tools and techniques, will be important to answer several of the priority questions identified here. Another important question raised was how to protect local cultural and social values and maintain indigenous engagement during problem formulation and identification of ecosystem protection goals. Addressing these questions will be challenging, but doing so promises to advance environmental sustainability in Oceania and globally.

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Effect-based trigger values for in vitro bioassays: Reading across from existing water quality guideline values

Cited by 86 publications

References 60 publications

Development of a bioanalytical test battery for water quality monitoring: Fingerprinting identified micropollutants and their contribution to effects in surface water

Development of a bioanalytical test battery for water quality monitoring: Fingerprinting identified micropollutants and their contribution to effects in surface water

Regulatory assessment and risk management of chemical mixtures: challenges and ways forward

Towards Sustainable Environmental Quality: Priority Research Questions for the Australasian Region of Oceania

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