Effect and Tolerability of the Combined Plant Extract (PAC) in Treatment of Acne Vulgaris

Abstract

This study was undertaken to identify a new combined plant extract (PAC) intended to treat in patients with acne vulgaris. We detected strong antibacterial activities of Pinus densiflora leaf extract (LP) against Propionibacterium acnes (P. acnes) and Staphylococcus epidermidis (S. epidermidis) and anti-inflammatory effect of Artemisia annua L. extract (AA). To develop more effective agents in multiple targeting of the physiopathology of acne, a combination of three plant extracts (PAC), Pinus densiflora leaf extract (LP), Artemisia annua L. extract (AA), and Citrus junos fruit juice (FC), indicated anti-proliferative effect on P. acnes-induced HaCaT cells proliferation, was prepared mixing at ratio 2:1:1. PAC with MIC values of 2.25 mg/ml and 0.75 mg/ml against P. acnes and S. epidermidis was more active than LP (2.50 mg/ml and 1.00 mg/ml), was the most active of three extracts. PAC also showed more effective anti-inflammatory activity than a single plant extract in P. acnes-treated NF, HaCaT cells and LPS-treated Raw 264.7 cells. And then, we evaluated sebosuppressive effect of PAC on differentiation and lipogenesis of sebocytes. Results showed that PAC reduced sebum production and IGF-1-stimulated proliferation on sebocytes. In addition, PAC (final 2%) applied in a test product clinically decreased skin oil content, sebum output level, and the number of closed comedones and open comedones in a period of 4 weeks. The results of the studies demonstrate that PAC is clinically effective with anti-bacterial, anti-inflammatory, sebum protective properties for acne prone skin. These results suggest that in patients with acne, PAC can provide adjunctive care by targeting pathological factors including bacterial proliferation, inflammation and sebum production.