Effect and Mechanism of ShiZhiFang on Uric Acid Metabolism in Hyperuricemic Rats

Wu, Yansheng; Yi-xing, Wang; Ou, Jiaoying; Wan, Qiang; Shi, Liqiang; Li, Yingqiao; He, Fangping; Wang, Huiling; He, Liqun; Gao, Jiandong

doi:10.1155/2018/6821387

Cited by 10 publications

(7 citation statements)

References 48 publications

(41 reference statements)

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“…The ShiZhiFang (SZF), a TCM formula which consists of plantago seeds/ Plantago asiatica L. (60 g), white mustard seeds/ Sinapis alba L. (30 g), vaccaria seeds/ Gypsophila vaccaria (L.) Sm 30 g, and abutilon seeds/ Malva verticillata L. (30 g), is a compound that our research group usually applied to the treatment of hyperuricemia and hyperuricemia-related diseases, such as urate nephropathy. The clinical efficacy and safety of lowering uric acid levels were confirmed in our previous clinical study [ 53 ]. In our later study to test the efficacy and mechanism of this formula upon metabolism of uric acid, we found that SZF may be involved in facilitating the transcription as well as expression of renal rOAT3 and rOAT1 and inhibiting serum XOD activity [ 53 ].…”

Section: Herbs That Reduce Uric Acid By Inhibiting Its Production and Enhancing Its Excretionsupporting

confidence: 57%

“…The clinical efficacy and safety of lowering uric acid levels were confirmed in our previous clinical study [ 53 ]. In our later study to test the efficacy and mechanism of this formula upon metabolism of uric acid, we found that SZF may be involved in facilitating the transcription as well as expression of renal rOAT3 and rOAT1 and inhibiting serum XOD activity [ 53 ].…”

Section: Herbs That Reduce Uric Acid By Inhibiting Its Production and Enhancing Its Excretionsupporting

confidence: 57%

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Traditional Chinese Herbal Medicine Plays a Role in the Liver, Kidney, and Intestine to Ameliorate Hyperuricemia according to Experimental Studies

Gao

2021

Evidence-Based Complementary and Alternative Medicine

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In the last few decades, hyperuricemia has drawn increasing attention owing to its global prevalence. Observational surveys have manifested that there is a relation between hyperuricemia and increased risks of hypertension, chronic kidney disease, cardiovascular events, metabolic disorders, end stage renal disease, and mortality. As alternatives, Traditional Chinese medicinal herbs have demonstrated concrete effects in mitigating hyperuricemia in different experiments. Researchers have made efforts to investigate the role of herbal medicine in attenuating hyperuricemia. This review focuses on traditional Chinese herbal medicines that have been reported to ameliorate hyperuricemia in experimental studies.

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Section: Herbs That Reduce Uric Acid By Inhibiting Its Production and Enhancing Its Excretionsupporting

confidence: 57%

Section: Herbs That Reduce Uric Acid By Inhibiting Its Production and Enhancing Its Excretionsupporting

confidence: 57%

Traditional Chinese Herbal Medicine Plays a Role in the Liver, Kidney, and Intestine to Ameliorate Hyperuricemia according to Experimental Studies

Gao

2021

Evidence-Based Complementary and Alternative Medicine

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“…Scientists suggested that inflammatory necrosis is associated with an innate immune response and sterile injury can also activate the innate immune system leading to unnecessary damage in normal tissue (Rock et al, 2010). Recently (Wu et al, 2018), it was realized that the activation of NLRP3 inflammasome promoted the secretion of IL-1β and IL-18 as well as triggered a new cell death which resulted from inflammation. Pyroptosis is an example of cell inflammatory necrosis which is characterized by the formation of a ruptured pore with a diameter ranging between 1.1 and 2.4 nm in the plasma membrane.…”

Section: Discussionmentioning

confidence: 99%

“…Studies indicate that gout-associated uric acid crystals stimulate GSDMD, which is dispensable for cell death as well as IL-1β release (Rashidi et al, 2019). From our previous study, it was evident that the renal tubules led to inflammatory injury via NLRP3 inflammasome activation (Wu et al, 2017) besides uric acid transporter down-regulation (Wu et al, 2018), in hyperuricemic kidneys. However, the knowledge on whether uric acid result in renal tubular epithelial cell pyroptosis is scanty.…”

Section: Introductionmentioning

confidence: 99%

Hyperuricemia triggers Renal Tubular Epithelial Pyroptosis by using ROS to activate the NLRP3 inflammasome

et al. 2022

Preprint

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Renal injury resulting from hyperuricemia has gained a lot of interest. Pyroptosis refers to inflammatory cell death. The activated caspase-1 cleavage, and the pivotal protein - GSDMD could have an association with the hyperuricemic kidney lesion pathogenesis. ROS is a vital NLRP3 inflammasome antagonist in various cells. We investigated the mechanism through which ROS stimulates NLRP3 to modulate pyroptosis in renal tubular epithelial cells as well as hyperuricemic rat kidneys. Methods: In vitro cultured renal tubular epithelial cells（NRK-52E cells）were incubated with a gradient concentration of uric acid for 24 hr to investigate the pyroptosis through flow cytometry. Next, we used the inhibitors of ROS, mitochondrial ROS, NLRP3 and Caspase-1 respectively to intervene in uric acid treated cells to analyse pyproptosis and activation of ROS- NLRP3 inflammasome signal pathway. Finally, we evaluated the mechanism of hyperuricemia triggering renal tubular epithelial pyroptosis in rat kidney tissues. Results: The levels of ROS and mitochondrial ROS, the mRNA and protein expression of pyroptosis-associated factors Caspase-1 (p45, p20/10), NLRP3, and GSDMD were upregulated in uric acid, the induced NRK-52E cells as well as hyperuricemic model kidneys. The inhibition of ROS, mitochondrial ROS, NLRP3, or caspase-1 in the uric acid-induced NRK-52E cells may help in controlling pyroptosis. The expression of mRNA and protein by the cytokines IL-18 and IL-1β also increased. Conclusions: Generally, hyperuricemia triggered renal tubular epithelial pyroptosis via excessive ROS to modulate NLRP3 inflammasome activation in uric acid stimulated renal tubular epithelial cells as well as the oxonic acid potassium induced hyperuricemia.

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“…The serum lipid peroxidation level was measured as described elsewhere (Samarghandian et al 2014). The serum levels of uric acid and nitric oxide (NO) were determined by calorimetric assay (Wu et al 2018;Van Beezooijen et al 1998). The serum levels of ceruloplasmin, zinc, copper, PGE 2 , MMP-3, TNF-α, IL-1β, and IL-6 were determined as described previously (Dai et al 2018).…”

Section: Biochemical Markersmentioning

confidence: 99%

Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis

et al. 2020

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Rheumatoid arthritis (RA) is an autoimmune disorder that affects the joint synovium. Anserine is a functional dipeptide containing methylhistidine and β-alanine, and is present in the brain and skeletal muscle of birds and mammals. Glucosamine is an amino sugar used in the synthesis of glycosylated proteins and lipids. We evaluated the effects of anserine and glucosamine on RA. Rats were assigned into the control group, RA group, anserine group (1 mg/kg), glucosamine group (200 mg/kg), or anserine plus glucosamine group (anserine, 1 mg/kg + glucosamine, 200 mg/ kg). Treatment was continued for 45 consecutive days and was administered orally. The serum levels of catalase, glutathione peroxidase (Gpx), superoxide dismutase (SOD), reduced glutathione (GSH), lipid peroxidation, uric acid, nitric oxide, ceruloplasmin, zinc, copper, prostaglandin E 2 (PGE 2 ), matrix metalloproteinase (MMP)-3, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 were assayed. The mRNA and protein levels of nuclear factor (NF)-κB and inducible nitric oxide synthase (iNOS) in synovial tissue were also determined. Anserine plus glucosamine significantly increased the catalase, SOD, Gpx, GSH, and zinc levels compared to the control, anserine, and glucosamine groups. Also, anserine plus glucosamine significantly reduced the PGE 2 , MMP-3, TNF-α, IL-1β, and IL-6 levels compared to the control, anserine, and glucosamine groups. Furthermore, anserine plus glucosamine significantly reduced the mRNA and protein levels of NF-κB and iNOS compared to the control, anserine, and glucosamine groups. Therefore, supplementation of anserine plus glucosamine shows therapeutic potential for RA.

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Effect and Mechanism of ShiZhiFang on Uric Acid Metabolism in Hyperuricemic Rats

Cited by 10 publications

References 48 publications

Traditional Chinese Herbal Medicine Plays a Role in the Liver, Kidney, and Intestine to Ameliorate Hyperuricemia according to Experimental Studies

Traditional Chinese Herbal Medicine Plays a Role in the Liver, Kidney, and Intestine to Ameliorate Hyperuricemia according to Experimental Studies

Hyperuricemia triggers Renal Tubular Epithelial Pyroptosis by using ROS to activate the NLRP3 inflammasome

Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis

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