Effect and mechanism of Qishen Yiqi Pills on adriamycin- induced cardiomyopathy in mice

Tong, Jiayi; Xu, Yingqi; Ye-ping, Bian; Shen, Xiang-bo; Lei, Yan; Zhu, Xinyi

doi:10.1016/s1875-5364(13)60093-x

Cited by 8 publications

(7 citation statements)

References 5 publications

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“…Moreover, myocardial apoptosis and myocardial fibrosis were detected to assess the cardiotoxicity induced by Doxo in mice. Previous studies reported that QYDP attenuate cardiac dysfunction in Doxo-induced cardiomyopathy, and the potential mechanism could be reduction of cardiac apoptosis [3]. Consistent with these studies, we also observed that QYDP could effectively attenuate Doxo-induced cardiac apoptosis.…”

Section: Discussionsupporting

confidence: 90%

“…In our study, M-mode echocardiography was carried out to assess LV function and anti-actinin-2 antibody staining by immunofluorescence was applied to show the structure of cardiac muscle fibers. Previous studies reported that QYDP attenuate cardiac dysfunction in Doxo-induced cardiomyopathy, which could be attributed to reduction of cardiac apoptosis [3]. Besides, QYDP was also reported to significantly improve Doxo-induced cardiac muscle fibers disruption and its effect might be attributed to the elevation in myocardial ATP content [4].…”

Section: Discussionmentioning

confidence: 99%

“…However, few studies have explored whether QYDP exerted an effect on doxorubicin (Doxo)-induced cardiotoxicity. Some results showed that QYDP could effectively improve Doxo-induced cardiac dysfunction [3,4]. However, its mechanism remains inconclusive.…”

Section: Introductionmentioning

confidence: 99%

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Qishen Yiqi Dropping Pills Ameliorates Doxorubicin-induced Cardiotoxicity in Mice via Enhancement of Cardiac Angiogenesis

Wang

Zhou

et al. 2019

Med Sci Monit

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Background Qishen Yiqi Dropping Pills (QYDP) is a Chinese traditional medicine that has been applied to treat coronary heart disease and ischemic heart failure in China. However, few studies have explored whether QYDP exerted an effect on doxorubicin (Doxo)-induced cardiotoxicity. Hence, in this study we investigated the effect of QYDP on cardiotoxicity induced by doxorubicin (Doxo) and its potential mechanism. Material/Methods Male C57BL/6 mice (20–25 g, 8–10 weeks old) were randomly assigned to 4 groups: Control group, QYDP group, Doxo group, and QYDP+Doxo group. The mice were intraperitoneal injected with Doxo weekly for 4 weeks to mimic the chronic toxicity. Four weeks after Doxo injection, echocardiography was applied to evaluate the left ventricular (LV) function, and the structure of the cardiac muscle fibers was analyzed with anti-actinin-2 antibody staining by immunofluorescence. Moreover, TUNEL staining and western blot analysis of Bax protein, Bcl-2 protein, and cleaved caspase-3 protein expression levels were conducted to explore whether QYDP exerted effect on cardiac apoptosis. In addition, Masson trichrome staining and western blot analysis of α-SMA protein expression levels were used to evaluate whether QYDP exerted an effect on cardiac fibrosis. Western blots and quantitative real-time polymerase chain reaction were applied to detect the vascular endothelial growth factor (VEGF) protein and mRNA levels in the myocardial tissue, and anti-CD31 antibody staining by immunohistochemistry was employed to explore whether QYDP exerted an effect on cardiac angiogenesis. Results QYDP effectively attenuated cardiac dysfunction and cardiac muscle fibers disruption in Doxo treated mice. Moreover, QYDP reduced myocardial apoptosis and myocardial fibrosis in Doxo treated mice, accompanied with elevated protein levels of VEGF and enhancement of myocardial microvessel density. Conclusions QYDP could protect against Doxo-induced cardiotoxicity, which may be closely associated with enhanced cardiac angiogenesis. Hence, QYDP could be a promising alternative for the treatment of Doxo-induced cardiotoxicity.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Qishen Yiqi Dropping Pills Ameliorates Doxorubicin-induced Cardiotoxicity in Mice via Enhancement of Cardiac Angiogenesis

Wang

Zhou

et al. 2019

Med Sci Monit

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“…Moreover, QSYQ protected against left ventricular remodeling induced by left anterior descending coronary artery ligation by attenuating the AngII–NADPH oxidase pathway (Li et al, 2014 ) or downregulating the RAAS pathway (Wang et al, 2015b ). Additionally, QSYQ could effectively improve the cardiac function in adriamycin-induced cardiomyopathy, and the probable mechanism of action could be the inhibition of myocardial cell apoptosis (Tong et al, 2013 ). In our study, we found that QSYQ could remarkably reduce cardiac apoptosis in TAC mice.…”

Section: Discussionmentioning

confidence: 99%

Cardioprotective Effects of QiShenYiQi Dripping Pills on Transverse Aortic Constriction-Induced Heart Failure in Mice

et al. 2018

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QiShenYiQi dripping pills (QSYQ), a traditional Chinese medicine, are commonly used to treat coronary heart disease, and QSYQ was recently approved as a complementary treatment for ischemic heart failure in China. However, only few studies reported on whether QSYQ exerts a protective effect on heart failure induced by pressure overload. In this study, we explored the role of QSYQ in a mouse model of heart failure induced by transverse aortic constriction (TAC). Twenty-eight C57BL/6J mice were divided into four groups: Sham + NS group, Sham + QSYQ group, TAC + NS group, and TAC + QSYQ group. QSYQ dissolved in normal saline (NS) was administered intragastrically (3.5 mg/100 g/day) in the Sham + QSYQ and TAC + QSYQ groups. In the Sham + NS and TAC + NS groups, NS was provided every day intragastrically. Eight weeks after TAC, echocardiography, and cardiac catheterization were performed to evaluate the cardiac function, and immunofluorescent staining with anti-actinin2 antibody was performed to determine the structure of the myocardial fibers. Moreover, TUNEL staining and Masson trichrome staining were employed to assess the effects of QSYQ on cardiac apoptosis and cardiac fibrosis. Western blots and real-time polymerase chain reaction (PCR) were used to measure the expression levels of vascular endothelial growth factor (VEGF) in the heart, and immunohistochemical staining with anti-CD31 antibody was performed to explore the role of QSYQ in cardiac angiogenesis. Results showed that TAC-induced cardiac dysfunction and disrupted structure of myocardial fibers significantly improved after QSYQ treatment. Moreover, QSYQ treatment also significantly improved cardiac apoptosis and cardiac fibrosis in TAC-induced heart failure, which was accompanied by an increase in VEGF expression levels and maintenance of microvessel density in the heart. In conclusion, QSYQ exerts a protective effect on TAC-induced heart failure, which could be attributed to enhanced cardiac angiogenesis, which is closely related to QSYQ. Thus, QSYQ may be a promising traditional Chinese medicine for the treatment of heart failure induced by pressure overload such as hypertension.

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“…However, the mechanism of QSYQ was mostly elucidated macroscopically by pharmacology indexes of animal experiments or clinical trials. For example, Tong et al [ 6 ] had reported that the myocardial protection function of QSYQ may relate to the reduction of myocardial cell apoptosis in adriamycin-induced cardiomyopathy animal model. The research of Cui et al [ 7 ] had shown that, by detecting clinical indexes which included the right ventricular end-diastolic volume (RVEDV), end-systolic volume (RVESV), stroke volume (SV), and right ventricular ejection fraction (RVEF), QSYQ could significantly improve the right heart function on patients undergoing valve replacement.…”

Section: Introductionmentioning

confidence: 99%

The Mechanism Research of Qishen Yiqi Formula by Module-Network Analysis

Zheng

Zhang

Qiao

2015

Evidence-Based Complementary and Alternative Medicine

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Qishen Yiqi formula (QSYQ) has the effect of tonifying Qi and promoting blood circulation, which is widely used to treat the cardiovascular diseases with Qi deficiency and blood stasis syndrome. However, the mechanism of QSYQ to tonify Qi and promote blood circulation is rarely reported at molecular or systems level. This study aimed to elucidate the mechanism of QSYQ based on the protein interaction network (PIN) analysis. The targets' information of the active components was obtained from ChEMBL and STITCH databases and was further used to search against protein-protein interactions by String database. Next, the PINs of QSYQ were constructed by Cytoscape and were analyzed by gene ontology enrichment analysis based on Markov Cluster algorithm. Finally, based on the topological parameters, the properties of scale-free, small world, and modularity of the QSYQ's PINs were analyzed. And based on function modules, the mechanism of QSYQ was elucidated. The results indicated that Qi-tonifying efficacy of QSYQ may be partly attributed to the regulation of amino acid metabolism, carbohydrate metabolism, lipid metabolism, and cAMP metabolism, while QSYQ improves the blood stasis through the regulation of blood coagulation and cardiac muscle contraction. Meanwhile, the “synergy” of formula compatibility was also illuminated.

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Effect and mechanism of Qishen Yiqi Pills on adriamycin- induced cardiomyopathy in mice

Cited by 8 publications

References 5 publications

Qishen Yiqi Dropping Pills Ameliorates Doxorubicin-induced Cardiotoxicity in Mice via Enhancement of Cardiac Angiogenesis

Qishen Yiqi Dropping Pills Ameliorates Doxorubicin-induced Cardiotoxicity in Mice via Enhancement of Cardiac Angiogenesis

Cardioprotective Effects of QiShenYiQi Dripping Pills on Transverse Aortic Constriction-Induced Heart Failure in Mice

The Mechanism Research of Qishen Yiqi Formula by Module-Network Analysis

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