Eff ects of Endophytes on Dysphania ambrosioides Heavy Metal Tolerance and Accumulation under Multi-heavy Metal Stress

Abstract

H igh concentration of h eavy metal (HM) is toxic to the plant and endophytes can improve host plants HM tolerance. In the present study, the role of endophytes FT2G7 (Phomopsis columnaris) and FT2G24 (Setophoma terrestris) on host plant Dysphania ambrosioides HM tolerance was evaluated under m ulti-HMs stress through pot experiments. The results showed that regardless at lower or higher multi-HMs stress, inoculation of isolate(s) improved host plants growth and HM tolerance through regulating their antioxidant capacities etc., and inoculation of multi-isolates (ME+) had better effects on host plants than inoculation of single isolate (SE+). Particularly, the malondialdehyde (MDA) of ME+ decreased 50% under the lower multi-HMs stress and the glutathione (GSH) increased 169.55% under the higher multi-HMs stress when compared with ME-. Both inoculation of single isolate and multi-isolates increased host plants HM accumulation when compared with non-inoculation. However, different isolates had different effects: under the lower multi-HMs stress, the accumulation of multi-HMs in ME+ plants was higher than that of FT2G7-inoculated plants but lower than that of F T2G24-inoculated plants, w ith a few e xceptions, such as Zn in underground parts of ME+ was higher than that of FT2G24-inoculated plants. The bioaccumulation factor (BAF) of ME+ was higher than that of SE+, but the translocation factor (TF) was lower than that of SE+, e xcept Pb of FT2G24 inoculated plants. Under the higher multi-HMs stress, Zn and Cd in ME+ plants were lower but Pb was higher than that of SE+, with a few exceptions, such as Pb in underground parts of ME+ plants was lower than that of FT2G24-inoculated plants. The BAF and TF of ME+ were lower than that of SE+, except that of Pb.

Trends in Harnessing Plant Endophytic Microbiome for Heavy Metal Mitigation in Plants: A Perspective

“…Several plant growths promoting bacteria from the genus Pseudomonas , Streptomyces , Bacillus, and Methylobacterium have been isolated with the potential of HM-tolerance and the mitigation of adverse effects of HMs on plant hosts [ 96 ]. Recently, Tang and coworkers [ 169 ] studied the prospects of endophytes, Phomopsis columnaris, and Setophoma terrestris isolated from Dysphania ambrosioides in bioaccumulation under multiple HM stresses and metal tolerances. The results showed that the beneficial effects of endophyte inoculation improved plant growth and HM tolerance via antioxidant mechanisms.…”

“…The results showed that the beneficial effects of endophyte inoculation improved plant growth and HM tolerance via antioxidant mechanisms. In addition, multi-isolate inoculation in the plant host had better effects than a single isolate on the host plant [ 169 ]. Further studies by Bibi et al [ 170 ] discussed the efficient biodegradation of Cr metal in the contaminated soil via fungal endophytes, and 114 fungal endophyte strains were isolated from different plant species, namely Zea mays , Beta vulgare , Saccharum officinarum , Ficus carica , Parthenium argentatum , Cucurbita maxima, and Colocasia esculenta .…”