Efektivitas Penambahan Serum Sapi Pesisir Fase Berahi Terhadap Pematangan Oosit Kerbau Secara in Vitro

Abstract

This study aims to determine the percentage of buffalo oocytes matured in TCM-199 medium supplemented bovine serum pesisir estrus phase in vitro. Furthermore, to improve the efficiency of in vitro embryo production in buffalo cattle with bovine serum pesisir supplementation estrus phase.Buffalo oocytes matured in TCM-199 medium in 5% CO2 incubator and each treatment was added bovine serum pesisir estrus phase with different concentrations (0%, 10% and 20%).Parameters observed in this study is the percentage of buffalo oocytes matured without the addition of bovine serum pesisir estrus phase, the percentage of mature oocytes by the addition of bovine serum pesisir estrus phase 10% and the percentage of buffalo oocytes were matured by the addition of bovine serum pesisir estrus phase 20% are in vitro.Percentage of buffalo oocytes matured with the addition of bovine serum pesisir phase estrus as much 20% showed a highly significant difference (P <0.01) which is 70.04% when compared with the maturation by the addition of 10% bovine serum pesisir is 56.00% and without bovine serum pesisir estrus phase is 36.96% are in Virto.Based on the results of this study concluded that supplementation with bovine serum pesisir phase concentration of 20% in the maturation medium TCM-199 significantly (P <0.01) in increasing the level of maturation of buffalo oocytes in vitro compared with the addition of bovine serum pesisir estrus phase and without the addition of 10% bovine serum pesisir (control).