Efeitos Terapêuticos Do Diodo Emissor De Luz - Led Em Mastites Lactacionais

Santos, Mônica Cristina Melo; Filho, Francisco da Costa Gomes; Nicolau, Renata Amadei

doi:10.18066/revunivap.v18i32.75

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…Among these, light interaction (external energy stimulus) with biological tissue 18 has shown to be effective as a modulator of cellular activity 19 due to its biomodulatory effects observed both in vitro and in vivo investigations. 20 Light emitting diodes (LEDs) represent an alternative source of light to lasers (light amplification by stimulated emission of radiation), and has been clinically approved for use in human trials once is classified as “non-significant risk” by the Food and Drug Administration due to low increase in temperature of the tissues during irradiation. 21 LED has been used due to their applicability in clinical and experimental studies and is also investigated as a potential light source in research with biomodulatory effects.…”

Section: Introductionmentioning

confidence: 99%

Red light accelerates the formation of a human dermal equivalent

et al. 2018

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Development of biomaterials' substitutes and/or equivalents to mimic normal tissue is a current challenge in tissue engineering. Thus, three-dimensional cell culture using type I collagen as a polymeric matrix cell support designed to promote cell proliferation and differentiation was employed to create a dermal equivalent in vitro, as well to evaluate the photobiomodulation using red light. Polymeric matrix cell support was prepared from porcine serous collagen (1.1%) hydrolyzed for 96 h. The biomaterial exhibited porosity of 95%, a median pore of 44 µm and channels with an average distance between the walls of 78 ± 14 µm. The absorption of culture medium was 95%, and the sponge showed no cytotoxicity to Vero cells, a non-tumor cell line. Additionally, it was observed that irradiation with light at 630 nm (fluency 30 J cm) leads to the cellular photobiomodulation in both monolayer and human dermal equivalent (three-dimensional cell culture system). It was also verified that the cells cultured in the presence of the polymeric matrix cell support, allows differentiation and extracellular matrix secretion. Therefore, the results showed that the collagen sponge used as polymeric matrix cell support and the photobiomodulation at 630 nm are efficient for the production of a reconstructed human dermal equivalent in vitro.

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Section: Introductionmentioning

confidence: 99%

Red light accelerates the formation of a human dermal equivalent

et al. 2018

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“…A temperatura controlada foi de 27 °C. deste resultado deve-se ao fato de que a água representa a maior percentagem do peso total da MEC in vivo (SANTOS et al, 2012). Portanto, a capacidade de retenção é fundamental para o crescimento celular, já que este é facilitado em materiais que absorvem mais fluidos (RODRIGUES, 2011).…”

Section: Ensaio De Absorçãounclassified

Derme reconstituída (equivalente) <i>in vitro</i>

Oliveira¹

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The development of biomaterials substitutes and/or equivalents to mimic normal tissue is a currently challenge in tissue engineering. Studies using cell monolayer culture presents limitations with respect to two-dimensional interactions between the cells, and experiments using animals cannot predict results in humans, due to the high viability, thus compromising their clinical relevance. In consequence, threedimensional cell culture (3D) using a biomaterial designed to promote cell proliferation and differentiation has been used to recreate the complexity of a normal tissue, allowing a larger and complex cellular interaction. Aiming to mimic the in vivo environment, the present work refers to create a reconstituted dermis (dermal equivalent) in vitro using collagen, the most abundant component of the dermis, as biological matrix, as support for human fibroblasts, as well evaluate the photobiomodulation with light at 630 nm. First, a sponge was prepared from serous 1.1% porcine collagen hydrolyzed for 96 h. The biomaterial was characterized by determination of its porosity, pore diameter, the fluid absorption and the biocompatibility assays, since these parameters are important to the cell proliferation and differentiation resulting in the in vitro tissue formation. The biomaterial showed porosity of 95.2%, with a median pore of 44 µM estimated by mercury porosimetry injection, and channels with an average distance between the walls of 7814 µM estimated by SEM. These values are considered as ideal for a biosupport fibroblast growth. The absorption of water and growth medium was 95%, and the sponge showed no cytotoxicity for the Vero cell line. Additionally, it was investigated the effect of irradiation in 3D culture with red light (dose 30 J cm-2), that showed photobiomodulation on the dose 30 J cm-2 , for culturing cells in monolayer and in the early-stage of the cell growth in three-dimensional culture. By confocal microscopy, it was verified that the cells cultured in the presence of the sponge (3D culture), allows differentiation and extracellular matrix secretion. Therefore, the results showed that the collagen sponge used as a biomaterial for cell support and the photobiomodulation at 630 nm and dose of 30 J cm-2 are efficient for the production of a reconstructed dermis (equivalent) in vitro.

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Efeitos Terapêuticos Do Diodo Emissor De Luz - Led Em Mastites Lactacionais

Cited by 2 publications

References 4 publications

Red light accelerates the formation of a human dermal equivalent

Red light accelerates the formation of a human dermal equivalent

Derme reconstituída (equivalente) <i>in vitro</i>

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