Efeitos quantitativos da estocagem de sangue periférico nas determinações do hemograma automatizado

Dalanhol, Michele; Barros, Morgana; Mazuchelli, Josmar; Silva, Paulo Henrique; Hashimoto, Yuichi; Largura, Álvaro

doi:10.1590/s1516-84842010005000011

Cited by 7 publications

(8 citation statements)

References 2 publications

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“…However, our findings differ from the results obtained by Dalanhol et al (2010), who describe a reduction in thrombocyte counts only after 48 hours of storage. Besides, other studies using dogs show that storing time did not interfere with this cell type counts (Coelho 2006, Oliveira et al 2010.…”

Section: Resultscontrasting

confidence: 99%

“…Prolonged contact with EDTA provokes cellular degeneration that can difficult or even disable cellular differentiation. Since neutrophils are the predominant lymphocyte type in Giant anteaters, with old samples, a super estimation of their number could occur (Coelho 2006, Dalanhol et al 2010. After 48h, there was a decrease in eosinophil and lymphocyte numbers, a fact that was also described by Turhan et al (2011) who reported lower lymphocyte numbers over time.…”

Section: Resultsmentioning

confidence: 82%

“…Blood, when extracted, has limited duration (Dalanhol et al 2010). Therefore, it is important to investigate the changes that take place during storage because when leading with wild animals, the immediate hematologic study is not always possible (Shanmugam et al 2008, Graesli et al 2014.…”

Section: Resultsmentioning

confidence: 99%

“…It is known that prolonged contact of cells with EDTA increases cellular fragility, leading to structural modifications or even cell destruction (Mafuvadze & Erlwanger 2007, Walencik & Witeska 2007, Dalanhol et al 2010, Almeida et al 2012, Faggio et al 2014. Nevertheless, decreasing anticoagulant concentration was not effective in order to avoid those changes, being observed in M1 and more evident in M2.…”

Section: Resultsmentioning

confidence: 99%

“…In mammals, the most indicated anticoagulant for haematology analysis is ethylenediaminetetraacetic acid (EDTA). However, it is known that prolonged contact of this product with blood cells can considerably alter the exam results, leading to wrong values that do not reflect the real animal's condition (Buttarello 2004, Dalanhol et al 2010, Silva et al 2013.…”

Section: Introductionmentioning

confidence: 99%

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Giant Anteater (Myrmecophaga tridactyla Linnaeus, 1758) of the brazilian cerrado: hematology and storage effect

Oliveira

Trentin²,

Vila

et al. 2017

Pesq. Vet. Bras.

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Giant Anteater (Myrmecophaga tridactyla Linnaeus, 1758) of the brazilian cerrado: hematology and storage effect. Pesquisa Veterinária Brasileira 37 (7): [773][774][775][776][777][778][779][780]. Laborató-rio Clínico Veterinário, Hospital Veterinário, Departamento de Medicina Veterinária, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Rodovia Goiânia Km 8, Campus Samambaia, Goiânia, GO, Giant Anteater (Myrmecophaga tridactyla) is a vulnerable species because of progressive habitat destruction, mostly affected by wildfires and car accidents. The increasing number of animals that are attended by wildlife rescue centres reinforces the need of knowledge about haematological parameters, especially for those that inhabit Brazilian cerrado biome. For this purpose and in order to establish reference values for this species and also to compare them with previous studies, haematological analysis of captive giant anteaters from Brazilian cerrado were performed. Moreover, the alterations of blood samples after 24 and 48 hours of storage at refrigeration temperatures (4 o C) and preserved with two different EDTA concentrations (5% and 10%) were studied. Means and standard deviations of haematological parameters analysed immediately after collection were: RBC: 2,07 x106/µL ± 0,40; hematocrit: 38,08%± 5,93; haemoglobin: 11.33g/dL±2.15; MCV:186.52 fL±21.72; MCHC: 29.68g/dL±2.56; MCH: 55.08pcg±5,94; total leucocytes: 8.142/µL±2.441; neutrophils: 5.913/µL±2.168; lymphocytes: 1.460/ µL±740; eosinophil: 522/µL±385; monocytes: 247/µL±176; thrombocytes: 123.458/ µL±31.362 and total plasma protein: 6.23g/dL±0.49. This data shows evidence of the existence of important differences between these values and others from other areas, either from Brazil or from other South American countries. Those variations might be connected to environment, genetic, nutritional and/or management factors. Regarding the storage effect analysis, it can be concluded that in giant anteaters, haematological analysis can be performed until 24h after collection without any significant alterations on the haematological parameters, except for thrombocytes. Concerning the different EDTA concentrations, it can be concluded that there are no quantitative differences in haematological variables. Nevertheless, relevant morphologic alterations in blood cells can be observed after a 24h storage period, being most noticeable in the leucocytes. Those alterations can lead to misinterpretation of the results, interfering diagnosis, prognosis and treatment. (6,23g/dL±0,49). Tais dados permitem afirmar que existem importantes diferenças entre os valores hematoló-gicos destes em relação aos animais provenientes de outras regiões, tanto do Brasil quanto de outros países da América do Sul. Provavelmente, tais divergências estão associadas a fatores ambientais, genéticos, nutricionais e/ou de manejo. Quanto à análise das amostras estocadas, conclui-se que, em tamanduás-bandeiras, as amostras para a realização de hemograma podem ser processadas até 24 horas após a...

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Section: Resultscontrasting

confidence: 99%

Section: Resultsmentioning

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Giant Anteater (Myrmecophaga tridactyla Linnaeus, 1758) of the brazilian cerrado: hematology and storage effect

Oliveira

Trentin²,

Vila

et al. 2017

Pesq. Vet. Bras.

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A functional assay using human whole blood and flow cytometry analysis to evaluate cytotoxicity and immunomodulatory effect of anti-Trypanosoma cruzi drugs

Lopes

Ribeiro

Teixeira-Carvalho

et al. 2023

Experimental Parasitology

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Glycemic Control and Muscle Damage in 3 Athletes With Type 1 Diabetes During a Successful Performance in a Relay Ultramarathon: A Case Report

Belli

Macedo

Scariot

et al. 2017

Wilderness & Environmental Medicine

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Ultramarathon races are fairly demanding and impose substantial physiological stress on healthy athletes. These competitions may thus be considerably more challenging for individuals with diabetes. This case study aims to describe glycemic control, muscle damage, inflammation, and renal function in 3 athletes with type 1 diabetes during a successful performance in a relay ultramarathon. The team completed the race in 29 hours and 28 minutes, earning third place. The total distance covered by each athlete was 68.7, 84.5, and 65.1 km. Most blood glucose levels showed that athletes were in a zone where it was safe to exercise (90-250 mg/dL or 5.0-13.9 mmol/L). Creatine kinase, lactate dehydrogenase, and aspartate aminotransferase serum levels increased 1.2- to 50.7-fold prerace to postrace, and were higher than the reference ranges for all the athletes postrace. Blood leukocytes, neutrophils, and serum C-reactive protein (CRP) increased 1.6- to 52-fold prerace to postrace and were higher than the reference ranges for 2 athletes after the race. Serum creatinine increased 1.2-fold prerace to postrace for all the athletes but did not meet the risk criteria for acute kidney injury. In conclusion, our main findings show evidence of satisfactory glycemic control in athletes with type 1 diabetes during a relay ultramarathon. Moreover, elevation of muscle damage and inflammatory biomarkers occurred without affecting renal function and challenging the maintenance of blood glucose among athletes. These findings are novel and provide an initial understanding of the physiological responses in athletes with type 1 diabetes during ultramarathon races.

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Efeitos quantitativos da estocagem de sangue periférico nas determinações do hemograma automatizado

Cited by 7 publications

References 2 publications

Giant Anteater (Myrmecophaga tridactyla Linnaeus, 1758) of the brazilian cerrado: hematology and storage effect

Giant Anteater (Myrmecophaga tridactyla Linnaeus, 1758) of the brazilian cerrado: hematology and storage effect

A functional assay using human whole blood and flow cytometry analysis to evaluate cytotoxicity and immunomodulatory effect of anti-Trypanosoma cruzi drugs

Glycemic Control and Muscle Damage in 3 Athletes With Type 1 Diabetes During a Successful Performance in a Relay Ultramarathon: A Case Report

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