Efeitos citotóxicos de anestesia local com lidocaína/ropivacaína em linhagens celulares de melanoma humano

Kang, Ding-kun; Zhao, Liyan; Wang, Hongli

doi:10.1016/j.bjan.2016.08.002

Cited by 14 publications

(4 citation statements)

References 27 publications

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“…Though the high concentration of plain ropivacaine solution showed a higher anesthetic score than low dosage, the highest concentration of 1% Ropivacaine HCl Injection (8.3 mg/mL of ropivacaine base) available in clinic is almost equal to the concentration of low dosage in the present study. Some evidence suggests that the cell toxicity effect of local anesthetics may be determined in a concentration-dependent manner from in vitro testing with specific cell lines, such as Schwann cells,17 human melanoma cells18 or rabbit intervertebral disc cells 19. A comparative advantage of using a sustained-release formulation is it will decrease the local concentration of un-bound anesthetic by holding it in reserve in MVR to reduce the local toxicity.…”

Section: Discussionmentioning

confidence: 99%

<p>Formulation and evaluation of multilamellar vesicles ropivacaine in pain management</p>

Kao

Lin

Gwathney

et al. 2019

IJN

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PurposeThe improvement of postoperative pain control plays an important role in recovery outcomes and patient satisfaction. Multilamellar vesicles ropivacaine, MVR, is being developed to sustain the release of ropivacaine in situ while maintaining the local concentration of ropivacaine within the therapeutic window.MethodsThese studies summarized the processes of MVR formulation development and the evaluation of its releasing profile in vitro and the pharmacokinetics and anesthetic effect in vivo.ResultsThe MVR demonstrates a sustained-release profile in an in vitro serum environment model after 24 hrs of incubation which translates in the in vivo rat pharmacokinetic profile of ropivacaine as a prolonged half-life that is 10-fold longer in duration than plain ropivacaine solution. The anesthetic effect of single-dose MVR is apparent by providing a prolonged analgesia effect compared to plain ropivacaine solution in an in vivo guinea pig pin-prick wheal model after a single intracutaneous injection. From a safety evaluation, MVR is well tolerated after a subcutaneously injection at a dose level of 20 mg/kg in rats, with no observable changes in clinical observation, body weight, organ weight, hematology and serum chemistry analysis.ConclusionThese results suggest that single administration of MVR is a promising candidate in postoperative pain management.

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Section: Discussionmentioning

confidence: 99%

<p>Formulation and evaluation of multilamellar vesicles ropivacaine in pain management</p>

Kao

Lin

Gwathney

et al. 2019

IJN

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“…In addition, they reveal the potential of LDC for the treatment of melanoma, in association with an antineoplastic agent. Previous studies pointed out the anticarcinogenic effect of LDC in different tumor models, such as breast [68], bladder [20], squamous and basal cell carcinoma [69], gastric cancer [28] and melanoma cell [19]. A recent review shows that LDC acts as a chemosensitizer to other antineoplastic agents, inhibiting the growth of tumors after single use at different concentrations through the regulation of epigenetic modifications, promotion of pro-apoptotic pathways and angiogenic inhibitor.…”

Section: In Vivo Antitumor Efficacy In a Melanoma Modelmentioning

confidence: 99%

“…Recently, several works have described the adjuvant anticancer action of local anesthetics such as LDC [19,20], bupivacaine [21][22][23][24] and ropivacaine [25]. LDC can suppress cancer cell growth (in vitro and in vivo) through several mechanisms: regulation of epigenetic changes, promotion of pro-apoptotic pathways and regulation of ABC transporters, also preventing metastasis and angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

Docetaxel and Lidocaine Co-Loaded (NLC-in-Hydrogel) Hybrid System Designed for the Treatment of Melanoma

et al. 2021

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Melanoma is the most aggressive skin carcinoma and nanotechnology can bring new options for its pharmacological treatment. Nanostructured lipid carriers (NLC) are ideal drug-delivery carriers for hydrophobic drugs, such as the antineoplastic docetaxel (DTX), and hybrid (NLC-in-hydrogel) systems are suitable for topical application. This work describes a formulation of NLCDTX in xanthan-chitosan hydrogel containing lidocaine (LDC) with anticancer and analgesia effects. The optimized nanoparticles encapsulated 96% DTX and rheological analysis revealed inherent viscoelastic properties of the hydrogel. In vitro assays over murine fibroblasts (NIH/3T3) and melanoma cells (B16-F10), human keratinocytes (HaCaT) and melanoma cells (SK-MEL-103) showed reduction of docetaxel cytotoxicity after encapsulation in NLCDTX and HGel-NLCDTX. Addition of LDC to the hybrid system (HGel-NLCDTX-LDC) increased cell death in tumor and normal cells. In vivo tests on C57BL/6J mice with B16-F10-induced melanoma indicated that LDC, NLCDTX, HGel-NLCDTX-LDC and NLCDTX + HGel-LDC significantly inhibited tumor growth while microPET/SPECT/CT data suggest better prognosis with the hybrid treatment. No adverse effects were observed in cell survival, weight/feed-consumption or serum biochemical markers (ALT, AST, creatinine, urea) of animals treated with NLCDTX or the hybrid system. These results confirm the adjuvant antitumor effect of lidocaine and endorse HGel-NLCDTX-LDC as a promising formulation for the topical treatment of melanoma.

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“…A hialuronidase aumenta a velocidade de absorção, diminue o desconforto da injeção subcutânea e aumenta a eficácia da anestesia local 10 ; o desoxicolato de sódio destrói os adipócitos e causa uma retração na pele, mostrando eficácia no tratamento da gordura localizada 11 ; l-carnitina, auxilia transformação de gordura em energia e participa do metabolismo de lipídeos 12 ; tripeptídeo, quebra das moléculas de gordura, facilitando sua remoção 13 ; trissilinol regula atividade dos fibroblastos aumentando síntese de colágeno, elastina e proteoglicanas 14 ; SAC (siloxanetriol alginate caffeine) produto que contribui para a firmeza e tonicidade da pele, auxiliando de forma natural o processo de eliminação da gordura localizada 11 . A lidocaína é indicado para anestesia local ou regional 15 .…”

Section: Introductionunclassified

Bichectomia associada ao slim face

Penoni¹,

Silva²

2022

J Multidiscip Dent

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A harmonização orofacial é uma especialidade da Odontologia que tem como objetivo a reabilitação estética e funcional das estruturas orofaciais. Dentre os procedimentos da harmonização orofacial está a bichectomia que é um procedimento cirúrgico que remove a bola de Bichat, tecido adiposo localizado na bochecha entre o músculo bucinador e masseter, tendo como objetivo diminuir o volume da mesma por estética ou função. O slim face se apresenta num kit, composto por seis produtos: hialuronidase, desoxicolato, l-carnitina, tripeptídeo, trissilinol, SAC (siloxanetriol alginate caffeine) e Lidocaína que quando manipulados de maneira homogênea e injetados na face resulta em reduzir a gordura localizada, regenerando o tecido conjuntivo, diminuindo a flacidez, ativando a quebra de gordura, promovendo a autoestima do paciente. Este trabalho teve como objetivo relatar um caso clínico de emagrecimento facial em uma paciente portadora de adiposidade facial que foi submetida ao tratamento de harmonização orofacial utilizando a técnica de bichectomia associada ao slim face. Os resultados obtidos, a remoção da bola de Bichat e a redução da gordura localizada, foram satisfatórios para o profissional e para o paciente. Concluiu-se que a bichectomia associada ao slim face promove o emagrecimento facial.

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Efeitos citotóxicos de anestesia local com lidocaína/ropivacaína em linhagens celulares de melanoma humano

Abstract: LAs have cytotoxic activity on human melanoma cell lines in a time-, concentration- and agent-dependant manner. Apoptosis in the cell lines was mediated through activity of caspases-3 and caspases-8.

Cited by 14 publications

References 27 publications

<p>Formulation and evaluation of multilamellar vesicles ropivacaine in pain management</p>

<p>Formulation and evaluation of multilamellar vesicles ropivacaine in pain management</p>

Docetaxel and Lidocaine Co-Loaded (NLC-in-Hydrogel) Hybrid System Designed for the Treatment of Melanoma

Bichectomia associada ao slim face

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