Efeito hepatoprotetor do consumo crônico de dieptanoína e trieptanoína contra a esteatose em ratos

Abstract

OBJETIVO: Avaliar o efeito do consumo crônico de di e trieptanoínas sobre a esteatose hepática (EH) em ratos. METODOLOGIA: Ratos Wistar submetidos à dieta AIN-93 com 0%, 30% ou 50% de substituição do óleo por óleo rico em di e trieptanoína, grupos TAGC(7)0, TAGC(7)30 e TAGC(7)50, respectivamente, por nove meses. O grupo-controle recebeu ração Labina®. Analisaram-se histologia e provas de função e lesão hepática, glicemia e perfil lipídico sérico. Realizaram-se análise de variância, teste F, teste de Dunnet e análises de regressão uni e multivariadas (p < 0,05). RESULTADOS: TAGC(7)0, TAGC(7)30 e TAGC(7)50 desenvolveram EH; 80% de casos graves no TAGC(7)0 contra 40% no TAGC(7)50. Os pesos absoluto (PAF) e relativo do fígado (PRF) foram maiores em TAGC(7)0 e TAGC(7)30 e a glicemia foi maior em TAGC(7)30 e TAGC(7)50, que no grupo-controle. Colesterol total, LDL-c, LDL-c/HDL-c e proteínas totais foram maiores no grupo-controle. O óleo experimental reduziu o PRF e determinou tendência de redução do peso corporal, PAF, percentual de lipídios hepáticos e graus de EH (GHE). As variáveis explicativas para GHE foram peso final, glicemia, albumina, HDL-c, LDL-c, LDL-c/HDL-c, VLDL-c e fosfatase alcalina. CONCLUSÕES: Sugere-se que di e trieptanoínas exerçam efeito hepatoprotetor contra a EH, em ratos, em uma feição dose-dependente.

Choline and Cystine Deficient Diets in Animal Models with Hepatocellular Injury: Evaluation of Oxidative Stress and Expression of RAGE, TNF-α, and IL-1β

“…This fact had already been identified in previous studies in different contexts [34–37], suggesting that the AIN-93 composition is inappropriate and justifying, therefore, our option for the present diet (Table 1), and which, independent of changes in diet composition, led to HS. One hypothesis to explain this liver damage is related to inadequate amounts of choline and sulfur-containing amino acids in the diet.…”

Metabolic dysfunctions promoted by AIN-93G standard diet compared with three obesity-inducing diets in C57BL/6J mice

“…Since the AIN-93G diet is used as a basis to produce hyperlipidic diets, the use of its original formulation as a control seems to be something interesting. However, animals fed this standard diet have shown hepatic steatosis and hyperlipidemia, leading researchers to question whether this type of formulation would be the best option for use as a lean control group ( Silva et al, 2008 ; Ataíde et al, 2009 ; Farias Santos et al, 2015 ). We hypothesize that ingestion of normocaloric diet AIN-93G promotes weight gain and metabolic dysfunctions similar to the hypercaloric diet, raising the possibility of using it as a control.…”

Obesity-Linked PPARγ Ser273 Phosphorylation Promotes Beneficial Effects on the Liver, despite Reduced Insulin Sensitivity in Mice

“…Previous studies have suggested that a diet consisting of a specific proportion of macronutrients, sulfur-containing amino acids, and lipotropic agents (known as the MNT diet, based on the AIN-93M formula) can lead to increased hepatocyte volume and fatty liver, as well as changes in serum markers such as cholesterol, glucose, and triglycerides [ 36 , 37 , 38 ]. Our study aimed to investigate the effects of the MNT diet on KI animals and found that it had a more pronounced impact on this group.…”