Efeito dos extratos aquoso e oleoso de Nim [Azadirachta indica A. Juss (Meliaceae)] na produção de patulina em maçãs contaminadas por Penicillium expansum
“…Several assays with other fungi and toxins have shown similar results. Fungitoxic effects indicate that Neem extracts block the biosynthesis pathway of aflatoxin in Aspergillus parasiticus [31]; citrinin in Penicillium citrinum [35]; penicillic acid in Penicillium cycplopium [33]; patulin in Penicillium expansum in vitro [34] and in contaminated apples [32].…”
Section: Resultsmentioning
confidence: 99%
“…The Neem (Azadirachta indica A. Juss) is a denseleaved tree of the Meliaceae family whose biologically active extracts are obtained from its leaves, seeds, fruits and trunk, with acknowledged multiple therapeutic, insecticide, nematicide, anti-microbial and fungicide qualities [24][25][26]. Several studies show that Neem water and oil extracts are toxic to fungi [27][28][29][30][31] and may result in the reduction or inhibition of mycotoxin production [32][33][34][35][36]. Great interest exists in verifying the effects of the plant's extract on the fungi Fusarium oxysporum f. sp.…”
Fungi of the genus Fusarium are well known plant pathogens, cause several vascular diseases and are producers of toxins. In vitro assays evaluated the effects of Neem (Azadirachta indica) oil on the diameter of colonies, dry weight, spore production, spore viability and production of Fusaric Acid toxin on Fusarium oxysporum f. sp. medicagenis and Fusarium subglutinans isolates. Effects of Neem oil were analyzed at concentrations 0.25%, 0.5% and 1% in Czapek Yeast Agar medium. The production of Fu- saric acid was determined by Thin Layer Chromatography and quantified by UV spectrophotometry. Neem oil showed inhibitory effects on the isolates tested, although they varied according to type of isolate and oil concentration. Neem oil was efficient in reducing the colonies’ diameter and dry weight in concentration-dependent manner. Neem oil was efficacious at higher concentration in the decrease of sporulation. Spore germination was affected by Neem oil when the spore was grown in Neem-contained medium as when the spore emerged from a culture in a Neem medium. Neem oil decreased and even inhibited the production of Fusaric acid by the assayed isolates. Since these isolates are plant pathogens and producers of Fusaric acid, Neem oil may be introduced as an integral item in the management of host plants
“…Several assays with other fungi and toxins have shown similar results. Fungitoxic effects indicate that Neem extracts block the biosynthesis pathway of aflatoxin in Aspergillus parasiticus [31]; citrinin in Penicillium citrinum [35]; penicillic acid in Penicillium cycplopium [33]; patulin in Penicillium expansum in vitro [34] and in contaminated apples [32].…”
Section: Resultsmentioning
confidence: 99%
“…The Neem (Azadirachta indica A. Juss) is a denseleaved tree of the Meliaceae family whose biologically active extracts are obtained from its leaves, seeds, fruits and trunk, with acknowledged multiple therapeutic, insecticide, nematicide, anti-microbial and fungicide qualities [24][25][26]. Several studies show that Neem water and oil extracts are toxic to fungi [27][28][29][30][31] and may result in the reduction or inhibition of mycotoxin production [32][33][34][35][36]. Great interest exists in verifying the effects of the plant's extract on the fungi Fusarium oxysporum f. sp.…”
Fungi of the genus Fusarium are well known plant pathogens, cause several vascular diseases and are producers of toxins. In vitro assays evaluated the effects of Neem (Azadirachta indica) oil on the diameter of colonies, dry weight, spore production, spore viability and production of Fusaric Acid toxin on Fusarium oxysporum f. sp. medicagenis and Fusarium subglutinans isolates. Effects of Neem oil were analyzed at concentrations 0.25%, 0.5% and 1% in Czapek Yeast Agar medium. The production of Fu- saric acid was determined by Thin Layer Chromatography and quantified by UV spectrophotometry. Neem oil showed inhibitory effects on the isolates tested, although they varied according to type of isolate and oil concentration. Neem oil was efficient in reducing the colonies’ diameter and dry weight in concentration-dependent manner. Neem oil was efficacious at higher concentration in the decrease of sporulation. Spore germination was affected by Neem oil when the spore was grown in Neem-contained medium as when the spore emerged from a culture in a Neem medium. Neem oil decreased and even inhibited the production of Fusaric acid by the assayed isolates. Since these isolates are plant pathogens and producers of Fusaric acid, Neem oil may be introduced as an integral item in the management of host plants
“…Os resultados se assemelham aos apresentados neste trabalho, pois para estudos empregando o etanol como extrator, os efeitos de MIC não são promissores. Outros autores ainda reafirmam a baixa ação de extratos etanólicos de neem frente a fungos tais como (ARROTEIA; MACHINSKI, 2007).…”
Section: Discussionunclassified
“…Inúmeras substâncias com atividade antibacterianas foram isoladas ao longo da história, dentre eles os óleos essenciais, os terpenos, triterpenos e flavonoides (DI STASI, 1996;BRASIL, 2002;SUBAPRIYA et al, 2005;SUBRA-MANIAN et al, 2005;CORDEIRO et al, 2006). Mediantede vias metabólicas secundárias os vegetais podem produzir alcaloides, flavonoides, isoflavonoides, taninos, cumarinas, glicosídeos, terpenos (CECHINEL FILHO;YUNES, 1998;ARROTEIA et al, 2007). Várias espécies de plantas têm sido pesquisadas em relação a produção dos terpenoides (CAN-TRELL et al, 2001) e fisalinas (PIETRO et al, 2000;JA-NUÁRIO et al, 2002).…”
Azadirachta indica A. Juss, conhecida popularmente como "Neem" é uma árvore indiana, pertencente à família Meliaceae. Apresenta diferentes propriedades terapêuticas, dentre elas, a atividade antibacteriana, antiparasitária, antifúngica e moluliscida. O objetivo do trabalho foi avaliar a atividade antifúngica do extrato bruto das folhas de A. indica frente às cepa padrão de Candida albicans ATCC 10231. Os extratos de A. indica foram preparados utilizando o álcool etílico 70%, acetato de etila e hexano como agentes extratores. Para a determinação da CIM (Concentração Inibitória Mínima) dos extratos foram realizadas as técnicas de macrodiluição em tubos e microdiluição em placa empregando a técnica do MABA (Microplate Alamar Blue Assay). Para o extrato bruto produzido com álcool etílico 70% e hexano, revelaram-se incapazes de inibir a cepa C. albicans mesmo na concentração de 2000 µg/mL, o extrato a base de acetato de etila foi o único capaz de exercer efeitos inibitórios em C. albicans ATCC 10231 na concentração de 2000µg/mL.
“…Extracts from neem (Azadirachta indica A. Juss), employed in pest control in plant cultures, (15,20) reveal bioactive substances in all parts of the tree, especially in its seeds (12). It has been shown that neem seeds oil has fungitoxic effects (10), whereas in vitro investigations revealed the inhibitory effects of neem extracts in the production of several mycotoxins (1,5,13,14). Results show that the production of ZEA in neem-less medium (control) was higher than that in extracts with NO (tests) ( Figure 1 and Table 1).…”
Zearalenone, a mycotoxin produced by fungi of the genus Fusarium, including F. graminearum, triggers reproduction disorders in certain animals and hyperestrogen syndromes in humans. Current research investigates three concentrations of neem oil extract (0.1, 0.25 and 0.5%) in reducing the production of zearalenone. Neem oil extract decreased zearalenone amount in the three concentrations but highest inhibition (59.05%) occurred at 0.1%
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.