Efeito Do Extrato De Piper Tuberculatum Sobre Adultos De Schistosoma Mansoni: Testes in Vitro E in Vivo

Abstract

Schistosomiasis is a severely neglected disease with a wide geographical distribution. It affects approximately 210 million people in the world and at least 800 million people live in risk areas. The search for new drugs to treat this parasitosis is significant due to the appearance of strains of the worm that are resistant to the currently available drugs. The retrieval of compounds extracted from plants that act on these parasites has increased scientific investigation of this subject. The present study demonstrates, in vitro and in vivo, the action of crude extract of Piper tuberculatum on adult Schistosoma mansoni. The extract was shown to be quite effective in the in vitro tests, causing soft tissue alterations and acting on the reproductive system of females and the mortality of the worms, with a greater effect on males. The in vivo experiment was performed with infected Mus musculus and a decrease in the number of eggs in the first and second oogram stages was found, suggesting action on oviposition. KEYWORDS: Schistosoma mansoni; Piper tuberculatum; in vitro; in vivo RESUMOEfeito do Extrato de Piper tuberculatum sobre adultos de Schistosoma mansoni: testes in vitro e in vivo A esquistossomose, doença negligenciada grave e de larga distribuição geográfica, atinge cerca de 210 milhões de pessoas no mundo e ao menos 800 milhões vivem em área de risco. A busca de novos medicamentos para o tratamento desta parasitose é relevante em razão do aparecimento de linhagens do verme resistentes aos fármacos disponíveis. A obtenção de compostos extraídos de plantas com ação sobre parasitos tem incrementado a investigação científica sobre este assunto. O presente trabalho mostra a ação in vitro e in vivo do extrato bruto de Piper tuberculatum sobre adultos de Schistosoma mansoni. O extrato mostrou-se bastante eficaz nos ensaios in vitro, provocando alterações tegumentares, tendo ação no sistema reprodutor das fêmeas e na mortalidade dos vermes

Antiparasitic effects of ethanolic extracts of Piper arboreum and Jatropha gossypiifolia leaves on cercariae and adult worms of Schistosoma mansoni

“…Simões et al . (2015) also demonstrated that the crude extract of P. tuberculatum is capable of acting internally in female worms, modifying some regions of the vitelline glands. However, the alterations in the female reproductive system are yet less explored; hence, the details mentioned in our study should be considered, as they may provide valuable information on new drug targets.…”

“…According to Matos-Rocha et al (2016), internal changes were also verified by transmission electron microscopy; the presence of vacuoles was found in the region of the syncytial matrix and of glycogen granules close to muscle fibres in adult worms of S. mansoni treated with the essential oil of Mentha x villosa leaves (500 μg mL −1 ), similar to the tegumentary dilations found in our study. Simões et al (2015) also demonstrated that the crude extract of P. tuberculatum is capable of acting internally in female worms, modifying some regions of the vitelline glands. However, the alterations in the female reproductive system are yet less explored; hence, the details mentioned in our study should be considered, as they may provide valuable information on new drug targets.…”

Parasitological and Biochemical Efficacy of the Active Ingredients of Allium sativum and Curcuma longa in Schistosoma mansoni Infected Mice

“…Authors recorded that the efficacy of praziquantel (PZQ) (the drug of choice) had been reduced as antischistosomal [ 13 , 14 ]. Scientists are still searching for antiparasitic drugs from natural origin for the development of new medications [ 15 , 16 ].…”

Evaluation of schistosomacidal activity of lannea schimperi and searsia longipes stem bark extracts

“…Schistosomias is endemic in tropical and sub-tropical regions and is estimated to kill 200 000 people per year and generally infects more than 210 million people (Simões et al, 2015;WHO, 2017). It was reported that around 800 million people globally are at high risk of getting Schistosomiasis (Simões et al, 2015).…”

“…Schistosomiasis infects more than 210 million peoples worldwide, whereby 91.4% occurs in African countries contributing to about 200 000 deaths annually (WHO, 2002;Rollinson et al, 2013;Simões et al, 2015). Furthermore, it's estimated that more than 800 million people are at risk of getting this disease globally (Simões et al, 2015). In Tanzania schistosomiasis is endemic all over the country; however the extent of endemicity is more pronounced in islands and regions bordering Lake Victoria.…”