Efeito da adição de Glutationa na função e estresse oxidativo em sêmen ovino criopreservado

Perez, Eduardo Gualtieri de Andrade; Nichi, Marcílio; Viana, Carlos Henrique Cabral; Silva, Rodrigo Otávio Correia da; Dalmazzo, Andressa; Góes, Paola Almeida de Araújo; Gurgel, João Rafael Chinait; Barnabé, Valquíria Hyppólito; Barnabé, Renato Campanarut

doi:10.11606/d.10.2009.tde-01022011-100638

Cited by 1 publication

(1 citation statement)

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“…No effect of glutathione was observed on ovine sperm characteristics cryopreserved using tris-egg yolk extender supplemented with 0, 1, 5 and 10 mM glutathione (Perez et al 2012) nor with 100, 200, and 400 mM (Câmara et al 2011). On the other hand, Mata-Campuzano et al (2014) observed that 5 mM glutathione had a positive impact on ovine semen quality because improved mitochondrial activity compared with 0.2 and 1 mM, but did not see increase in farrow rates sheep after artificial insemination.…”

Section: Groupsmentioning

confidence: 99%

Effect of different cryopreservation extenders added with antioxidants on semen quality and in vitro embryo production efficiency in cattle

Silva

Leão

Pádua

et al. 2021

An. Acad. Bras. Ciênc.

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To evaluate the addition of antioxidants in extenders on post-thaw bovine semen quality and in vitro embryo production effi ciency. Six semen samples were collected from fi ve Holstein bulls. In the experiment I, the samples were diluted with AndroMed® and Bovimix® and added antioxidants glutathione (1.5 and 2.5 mM) and melatonin (0.5 and 1.0 mM). In the experiment II, the best treatments obtained in experiment I were used for in vitro fecundation. Glutathione did not improve sperm viability. Melatonin had a negative effect on semen characteristics. Andromed® showed better results in sperm kinetics parameters. Bovimix® was more effi cient in maintaining cell integrity parameters. Signifi cant correlation was found between sperm kinetics parameters and between cell integrity parameters. For in vitro embryo production, after oocyte selection, maturation, fertilization and cultivation were performed using the four treatments previously evaluated. Andromed® was more effi cient in the cleavage rate, no effect of the addition of glutathione. However, the addition of 2.5 mM glutathione in the Bovimix® improved the cleavage rate. There was a signifi cant moderate correlation between cleavage rate and sperm kinetic characteristics. Glutathione did not improve sperm viability. Melatonin reduced the maintenance of sperm characteristics. Andromed® was more effi cient in in vitro embryo production and no effect of glutathione was found in this extender. Addition of 2.5 mM glutathione in the Bovimix® extender provided a higher cleavage rate.

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Section: Groupsmentioning

confidence: 99%