Efects of growth hormone and cadmium on the transcription regulation of two metallothionein isoforms

Vergani, Laura; Lanza, Cristina; Borghi, C.; Scarabelli, Laura; Panfoli, Isabella; Burlando, Bruno; Dondero, Francesco; Viarengo, Aldo; Gallo, Gabriella

doi:10.1016/j.mce.2006.08.010

Cited by 28 publications

(28 citation statements)

References 56 publications

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“…In this context, the MT concentration in controls and production of TH showed clear correlations. This observation was in agreement with a previous report that demonstrated possible interactions between TH production and MT synthesis in fish hepatic cells (Vergani et al, 2007). Changes in liver structure were also described during metamorphosis (Cohen, 1970).…”

Section: Decontamination Phasesupporting

confidence: 93%

Recovery capabilities of Xenopus laevis after exposure to Cadmium and Zinc

et al. 2015

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Section: Decontamination Phasesupporting

confidence: 93%

Recovery capabilities of Xenopus laevis after exposure to Cadmium and Zinc

et al. 2015

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“…First strand cDNA was synthesized from 1 lg of RNA [5]. The gene expression levels were quantified by quantitative RT-PCR (q-PCR) using Chromo4 TM System PCR apparatus and iTaq SYBR Green Supermix (Biorad, Milano, Italy) [27]. PPAR-d primers (FWD: 5 0 -AATGCCTACCTGAAAAACTTCAAC-3 0 and REV: 5 0 -TGCCTGCCACAGCGTCTCAAT-3 0 ) were designed ad hoc starting from the coding sequences of Rattus norvegicus.…”

Section: Rna Extraction and Real-time Rt-pcrmentioning

confidence: 99%

Direct effects of iodothyronines on excess fat storage in rat hepatocytes

Grasselli

Voci

Canesi

et al. 2011

Journal of Hepatology

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“…Metallothioneins are a class of low-molecular weight metal-binding proteins involved in scavenging of free radicals (Chung and West 2004) storage and metabolism of essential metals and detoxification of toxic metals (Vergani et al 2007). In the CNS, three MT isoforms are present that show distinct patterns of expression.…”

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confidence: 99%

Neuroprotective mesenchymal stem cells are endowed with a potent antioxidant effect in vivo

Lanza

Morando

Voci

et al. 2009

Journal of Neurochemistry

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1These authors contributed equally to this study.Abbreviations used: CAT, catalase; CM, conditioned medium; EAE, experimental autoimmune encephalomyelitis; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GFAP, glial fibrillary acid protein; GST, glutathione-S-transferase; MOG, myelin oligodendrocyte glycoprotein peptide; MS, multiple sclerosis; MSC, mesenchimal stem cell; MT, metallothionein; NeuN, neuronal nuclei; PARP-1, poly(ADP-ribose) polymerase-1; PBS, phosphate-buffered saline; RPMI, Royal Park Memorial Institute; SOD, superoxide dismutase. AbstractExperimental autoimmune encephalomyelitis (EAE), an animal model for human multiple sclerosis, is characterized by demyelination, inflammation and neurodegeneration of CNS in which free radicals play a role. Recently, the efficacy of murine mesenchimal stem cells (MSCs) as treatment of EAE induced in mice by the encephalitogenic peptide MOG(35-55) was demonstrated. The present study analyzed some markers of oxidative stress, inflammation/degeneration and apoptosis such as metallothioneins (MTs), antioxidant enzymes (superoxide dismutase, catalase and glutathione-S-transferase), poly(ADP-ribose) polymerase-1 and p53 during EAE progression and following MSC treatment. Expression of the three brain MT isoforms increased significantly in EAE mice compared with healthy controls, but while expression of MT-1 and MT-3 increased along EAE course, MT-2 was up-regulated at the onset, but returned to levels similar to those of controls in chronic phase. The changes in the transcription and activity of the antioxidant enzymes and in expression of poly(ADP-ribose) polymerase-1 and p53 showed the same kinetics observed for MT-1 and MT-3 during EAE. Interestingly, i.v. administration of MSCs reduced the EAE-induced increases in levels/activities of all these proteins. These results support an antioxidant and neuroprotective activity for MSCs that was also confirmed in vitro on neuroblastoma cells exposed to an oxidative insult.

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Efects of growth hormone and cadmium on the transcription regulation of two metallothionein isoforms

Cited by 28 publications

References 56 publications

Recovery capabilities of Xenopus laevis after exposure to Cadmium and Zinc

Recovery capabilities of Xenopus laevis after exposure to Cadmium and Zinc

Direct effects of iodothyronines on excess fat storage in rat hepatocytes

Neuroprotective mesenchymal stem cells are endowed with a potent antioxidant effect in vivo

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