Efecto del tratamiento previo de ácido diluido - peróxido en la hierba napier (Pennisetum purpureum schumach) para mejorar la reducción Producción de azúcar por hidrólisis enzimática

Abstract

The napier grass (Pennisetum purpureum Schumach.) is a potential feedstock biomass for conversion to ethanol, bio-energy or high added value products. Variation in prehydrolysis parameters (time, acid strength, biomass %, particle size) and enzymatic saccharification conditions were examined for conversion of napier grass into fermentable sugars. A pretreatment of the water insoluble substrate (WIS H 2 SO 4 ) at 15.1 % of reducing sugar yield (RSY) was done with peroxide (H 2 0 2 ) at 122 °C. The relationship between RSY and the acid process parameters are described by a mathematical model derived from the experimental data.The peroxide pretreatment at 0.7 % (w/w), 4.75 mm particle size, 8% (w/v) biomass concentration, improved the RSY production from enzymatic hydrolysis in 97.6 % after 75 min. The enzymatic hydrolysis produced 287.81 mg/(g initial dry sample) of glucose and 245.81 mg/(g initial dry sample) of Xylose. Therefore, it was concluded that combination peroxide -acid pretreatment is an effective and environmentally friendly method for the enzyme hydrolysis of napier grass.