Efecto del Hongo Isaria fumosorosea Wize Sobre la Herbivoría por los Adultos del Escarabajo de Margen Amarillo, Microtheca ochroloma Stal (Coleoptera: Chrysomelidae)

Herrera, C. Gámez; Niño, Angie A.; Avery, Pasco B.; Cave, Ronald D.

doi:10.5377/ceiba.v54i2.3227

Cited by 3 publications

(7 citation statements)

References 18 publications

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“…Radial growths at day 15 for all EPF subjected to 50% concentration of bok choy and turnip leaf extracts were greater than radial growth on PDA without leaf extract; however, radial growths of C. fumosorosea Apopka strain and B. bassiana GHA strain exposed to the turnip leaf extract were greater at the 25% concentration level (Figure 3). Although interesting, these findings are contrary to what we expected to observe based on a previous in vivo study where the yellowmargined leaf beetle adult was rarely infected by C. fumosorosea Apopka strain while feeding on bok choy leaves sprayed with the fungus [17,18]. However, in corroboration with the findings in our study, Lin et al [41,42] observed that the herbivore-induced plant volatiles (HIPVs) emitted from the crucifer Arabidopsis thaliana (L.) Heynh, while its leaves were fed on by the aphid L. erysimi, were stimulatory to spore germination and appressorial formation of L. lecanii, which are requisites for pathogenicity of the fungal infection.…”

Section: Discussioncontrasting

confidence: 99%

“…Although speculative and interesting, this hypothesis requires further study to confirm this specific scenario. However, the yellowmargined leaf beetle was observed to be unaffected by the presence of C. fumosorosea propagules after feeding on bok choy leaf [17,18]. Researchers investigating similar tritrophic interactions have concluded that plant exudates and HIPVs released on the leaf phylloplane can affect: (1) the impact of insect fungal pathogens by enhancing or decreasing persistence on the leaf surface [35,41,46], (2) the encounter rate between the insect and the pathogen [47], (3) insect susceptibility to disease [33,[48][49][50][51][52].…”

Section: Discussionmentioning

confidence: 93%

“…It is important to determine the potential effect of a host plant's secondary allelochemicals on the growth of an EPF contained in a fungal biopesticide product in vitro, prior to it being applied under greenhouse or field conditions, in order to maximize the efficacy of any fungal biopesticide. In our case, the in vivo study was conducted first where we observed that the yellowmargined leaf beetle feeding on the bok choy leaves was difficult to induce mortality with C. fumosorosea applied at 2× the label rate [17,18]. We hypothesized that the growth and subsequent pathogenicity of the fungus may be negatively influenced by the presence of glucosinolates in the hemocoel sequestered per os from the leaves during beetle herbivory.…”

Section: Discussionmentioning

confidence: 99%

“…The ingestion and sequestration of allelochemical substances by arthropods that consume the brassicaceous plant tissue could play a role in these specialist herbivores rarely becoming infected by EPF [11,27,28]. Gámez Herrera et al [17] and Montemayor et al [18], who investigated the efficacy of commercially formulated C. fumosorosea applied against yellowmargined leaf beetles on bok choy (Brassica rapa subspecies chinensis) plants, observed in vivo, that an application rate 2× the label rate resulted in only 35% and 4% mortality of first instars and adults, respectively. Therefore, it appears that the efficacy of C. fumosorosea may be hindered due to the sequestration of glucosinolates by the beetle from feeding on the bok choy leaves.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Effects of Leaf Extracts from Brassica rapa on the Growth of Two Entomopathogenic Fungi

Cerritos-Garcia

Avery

Martini

et al. 2021

JoF

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This study aimed to determine the inhibitive or stimulatory effects of leaf extracts from two Brassica rapa subspecies on the hyphal growth of two well-known entomopathogenic fungi, Cordyceps fumosorosea and Beauveria bassiana. Extract concentrations of 50, 25, and 10% w/v based on leaf fresh weight were prepared from turnip (B. rapa subspecies rapa) and bok choy (B. rapa subspecies chinensis) leaves. Each concentration was individually incorporated into potato dextrose agar plates for in vitro bioassays. The center of each plate was inoculated with 20 µL of a fungal suspension that was allowed 24 h to soak into the agar before sealing the plates and incubating them at 25 °C under a 14-h photophase. The fungal colony perimeter was marked 5 days after inoculation on two perpendicular lines drawn on the bottom of each plate. Radial colony growth was measured from 4 marks per plate 5, 10, and 15 days later. Radial growth rates for both fungi were 1.3–2.0 and 0.9–1.4 times faster with bok choy and turnip extracts, respectively, at the 25% and 50% concentrations compared to the no-extract control treatment. Therefore, bok choy and turnip leaf extracts can stimulate entomopathogenic fungus growth within 15 days. Biochemical compounds in the extracts include sesquiterpenes, α-copaene, β-selinene, γ-gurjunene, calamenene, cubenene, and α-calacorene.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro Effects of Leaf Extracts from Brassica rapa on the Growth of Two Entomopathogenic Fungi

Cerritos-Garcia

Avery

Martini

et al. 2021

JoF

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“…Consumption by the cowpea leaf beetle, Ootheca mutabilis Shalberg, treated with B. bassiana CPD 3 in feeding assays was significantly reduced within 2 days post-treatment, and this trend continued until 7 days when all the beetles died [44]. Most recently, this trend was also observed where the yellowmargined leaf beetle, Microtheca ochroloma Stål, consumption of bok choy leaves treated with Ifr was significantly reduced compared to the untreated plants [45]. …”

Section: Discussionmentioning

confidence: 99%

Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

Avery

Hunter

Hall

et al. 2016

Insects

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The efficacy of topical, leaf residue, and soil drench applications with Isaria fumosorosea blastospores (Ifr strain 3581) was assessed for the management of the citrus root weevil, Diaprepes abbreviatus (L.). Blastospores of Ifr were applied topically at a rate of 107 blastospores mL−1 on both the larvae and adults, and each insect stage was incubated in rearing cups with artificial diet at 25 °C, either in the dark or in a growth chamber under a 16 h photophase for 2 weeks, respectively. Percent larval and adult mortality due to the infection of Ifr was assessed after 14 days as compared to untreated controls. Leaf residue assays were assessed by feeding the adults detached citrus leaves previously sprayed with Ifr (107 blastospores mL−1) in Petri dish chambers and then incubating them at 25 °C for 2–3 weeks. Efficacy of the soil drench applications was assessed on five larvae feeding on the roots of a Carrizo hybrid citrus seedling ~8.5–10.5 cm below the sterile sand surface in a single 16 cm × 15.5 cm pot inside a second pot lined with plastic mesh to prevent escapees. Drench treatments per pot consisted of 100 mL of Ifr suspension (107 blastospores mL−1), flushed with 400, 900, or 1400 mL of water compared to 500, 1000, and 1500 mL of water only for controls. The mean concentration of Ifr propagules as colony forming units per gram (CFUs g−1) that leached to different depths in the sand profile per treatment drench rate was also determined. Two weeks post-drenching of Ifr treatments, larvae were assessed for percent mortality, size differences, and effect of treatments in reducing feeding damage to the plant root biomass compared to the controls. Topical spray applications caused 13 and 19% mortality in larvae and adults after 7 days compared to none in the control after 14 days, respectively. Adults feeding on a single Ifr treated leaf for 24 h consumed less than the control, and resulted in 100% mortality 35 days post-treatment compared to 33% in the untreated control. Although offered fresh, untreated leaves after 24 h, only adults in the control group consumed them. Ifr CFUs g−1 were isolated 8.5–10.5 cm below the sand surface for the 1000 and 1500 mL drench rates only, resulting in 2%–4% larval mortality. For all the Ifr drench treatments, no differences were observed in percent larval mortality and size or the effect of treatments in reducing feeding damage to the plant root biomass compared to the controls. These results suggest that the foliar application of Ifr may be an efficient biocontrol strategy for managing adult populations of D. abbreviatus; potential alternative larval management strategies are discussed.

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The Consumption and Survival Rate of Lilioceris cheni (Coleoptera: Chrysomelidae) on Air Potato Leaves Exposed to Cordyceps javanica (Hypocreales: Cordycipitaceae)

Kariuki

Lovo

Price

et al. 2022

Florida Entomologist

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Efecto del Hongo Isaria fumosorosea Wize Sobre la Herbivoría por los Adultos del Escarabajo de Margen Amarillo, Microtheca ochroloma Stal (Coleoptera: Chrysomelidae)

Cited by 3 publications

References 18 publications

In Vitro Effects of Leaf Extracts from Brassica rapa on the Growth of Two Entomopathogenic Fungi

In Vitro Effects of Leaf Extracts from Brassica rapa on the Growth of Two Entomopathogenic Fungi

Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

The Consumption and Survival Rate of Lilioceris cheni (Coleoptera: Chrysomelidae) on Air Potato Leaves Exposed to Cordyceps javanica (Hypocreales: Cordycipitaceae)

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