Efecto del análogo de L-carnitina, la b-hidroxifosfocarnitina sobre el metabolismo de Nocardia brasiliensis

Abstract

Objectives: To determine whether β-HFC, in addition to its immunomodulatory effect as a therapeutic alternative in the combat of the mycetoma has an effect on the biochemical activity of N. brasiliensis. Methodology: determination of bacterial growth using N. brasilensis in the presence of β-HFC and biochemical analysis of its metabolism. Contribution: The immunomodulatory function of β-HFC in the treatment of chronic infections has recently been studied but it is not known whether it also has any antimicrobial effect, so in this paper the direct effect of β-HFC in N. brasiliensis will be elucidated in part.

Effect of β − hydroxy − γ -aminophosphonate (β − HPC) on the hydrolytic activity of Nocardia brasiliensis as determined by FT−IR spectrometry

“…Therefore, the stronger signal of β-HPC equalized the hydrolysis of such compounds at 1650 to 1400 cm −1 , but perhaps the phosvitin catabolism that has a remarkable resistance to hydrolysis, thanks to the β-HPC the amide II stretching vibration increased, as well as the heavier phosphate signals 10.3389/fmicb.2023.1089156 at 1071 and 520 cm −1 , also a signal of increased hydrolysis (Huang et al, 2019;Zhao et al, 2021;Figure 5, 6D). N. brasiliensis + β-HPC consumed proteins, lipids and carotenes, as witnessed by the colorless supernatant after growth, compared to N. brasiliensis, which maintained a yellowish hydrolyzate (data not shown) (Martínez-Robles et al, 2019). In this particular substrate, statistical summaries placed N. brasiliensis at 187 µL and N. brasiliensis + β-HPC at 93.75 µL instead of PCA.…”