Efecto de la temperatura en el pre-acondicionamiento de plantas in vitro donantes de piña para un protocolo de crioconservación de ápices

Villalobos-Olivera, Ariel; Rodríguez, J. María Tabares; Bernabe, N.; Souza, Fernanda Vidigal Duarte; Olmedo, Justo González; Montero, Miguel

doi:10.17660/actahortic.2019.1239.14

Cited by 2 publications

(6 citation statements)

References 17 publications

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“…Therefore, this study investigates in detail, for the first time, the morpho-anatomical and physiological characteristics of cryo-derived pineapple plants during acclimatization. The results reported here support the use of the droplet-vitrification cryopreservation procedure described by Souza et al [10] and modified by Villalobos et al [12] for the establishment of cryopreserved pineapple gene bank collections.…”

Section: Introductionsupporting

confidence: 84%

“…Subculture in fresh culture medium was performed every 45 days. After 45 days of subculture and before cryopreservation, shoots were preconditioned for 45 days in a liquid MS culture medium containing 100 mg•L −1 myoinositol, 1.0 mg•L −1 thiamine and 30 g•L −1 sucrose at a pH of 5.7, as defined by Villalobos-Olivera et al [12].…”

Section: Plant Materials and Stock Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Cryopreservation methods have already been developed for pineapple shoot tips [5,[7][8][9][10][11][12][13]. The vitrification-based cryopreservation method is the most widely applied for cryopreserving pineapple shoot tips [5,[10][11][12][13]. In this method, precultured shoot tips are exposed to a highly concentrated plant vitrification solution (PVS) before being directly immersed in liquid nitrogen (LN) [2,12,13].…”

Section: Introductionmentioning

confidence: 99%

“…The vitrification-based cryopreservation method is the most widely applied for cryopreserving pineapple shoot tips [5,[10][11][12][13]. In this method, precultured shoot tips are exposed to a highly concentrated plant vitrification solution (PVS) before being directly immersed in liquid nitrogen (LN) [2,12,13]. To date, the droplet-vitrification technique has been shown to be the most effective cryopreservation method across diverse genotypes.…”

Section: Introductionmentioning

confidence: 99%

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Morpho-Anatomical and Physiological Assessments of Cryo-Derived Pineapple Plants (Ananas comosus var. comosus) after Acclimatization

et al. 2023

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Studies on the morpho-physiology of cryo-derived pineapple plants after acclimatization have been quite limited. Therefore, in the present study, the morpho-anatomical and physiological characteristics of cryo-derived Ananas comosus var. comosus ‘MD-2’ plants after acclimatization were investigated. Plants obtained from cryopreserved and non-cryopreserved shoot tips, as well as in vitro stock cultures (control), showed similar morphological development (viz. plant height, number of leaves, D leaf length, D leaf width, D leaf area, diameter of stem base, number of roots, plant fresh weight and plant dry weight) to conventionally micropropagated and non-cryopreserved plants. The pineapple plantlets developed efficient anatomical leaf structures that allowed them to adapt to the transition process from in vitro to ex vitro. In all groups of plants, the content of water and chlorophylls (a, a + b, a/b) decreased during the first 15 days of acclimatization and then remained constant until the end of the evaluation. The mesophilic succulence index increased to its maximum value after 15 days, then decreased and remained constant up to 45 days. Although physiological indicators fluctuated during the 45 days of acclimatization, no differences were observed in any of the indicators evaluated when plantlets obtained from cryopreserved shoot tips were compared with controls. The results of the plants from cryopreserved shoot tips show that they switched from C3 to Crassulacean acid metabolism, which denoted metabolic stability during acclimatization.

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Section: Introductionsupporting

confidence: 84%

Section: Plant Materials and Stock Culturesmentioning

confidence: 99%