Efecto de diferentes tipos de sustratos y auxinas en el establecimiento ex vitro de segmentos nodales de arándano Var. Biloxi

Abstract

Micropropagation of Vaccinium spp. using Murashige Skoog (MS) and benzylaminopurine (BAP) has been successfully developed. This work aimed to determine the effect of different types of substrates and auxins (naftalen acetic acid NAA, indol acetic acid IAA y, indol butyric acid IBA) on ex vitro establishment and rooting of nodal segments of V. corymbosum Var. Biloxi to create a donor bank of blueberry plants. The influence of five different substrates and three types of auxins using five concentrations was assessed for a better nodal segment ex vitro establishment. The 40 % coconut fiber substrate, 20 % pomine + 40 % peat improved ex vitro establishment of V. corymbosum Var. Biloxi nodal segments. Otherwise, using 100 ppm of NAA the ex vitro establishment of V. corymbosum Var. Biloxi nodal segments increased. These results will facilitate the conformation of a donor bank of youth plants of V. corymbosum, increasing possibilities of in vitro establishment for massive micropropagation. Keywords: acclimatization, eriaceae, hormones, Vaccinum.