Efect of zinc and iron deficiency on phytos1derophore release in wheat genotypes differing in zinc efficiency

Cakmak, Smail; Gülüt, Kemal; Marschner, H.; Graham, Robin D.

doi:10.1080/01904169409364706

Cited by 172 publications

(25 citation statements)

References 36 publications

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“…Measured exudation rates are in a comparable range with release rates of Fe deficient wheat (Cakmak Triticum aestivum cv Aroona, 25 days old, 1.14 nmol g −1 s −1 ) and other grasses reported in the literature (e.g. Clark Hordeum vulgare cv Europa, 19 days old, 3.1 nmol g −1 s −1 ) with values from literature here being converted to SI units for better comparability.…”

Section: Resultssupporting

confidence: 63%

Accurate LC‐ESI‐MS/MS quantification of 2′‐deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a ¹³C₄‐labeled internal standard

et al. 2014

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For the first time the phytosiderophore 2'-deoxymugineic acid (DMA) could be accurately quantified by LC-MS/MS in plant and soil related samples. For this purpose a novel chromatographic method employing porous graphitic carbon as stationary phase combined with ESI-MS/MS detection in selected reaction monitoring was developed. Isotope dilution was implemented by using in-house synthesized DMA as external calibrant and ¹³C₄-labeled DMA as internal standard (concentration levels of standards 0.1-80 μM, determination coefficient of linear regression R² > 0.9995). Sample preparation involved acidification of the samples in order to obtain complete dissociation of metal-DMA complexes. Excellent matrix related LOD and LOQ depending on different experimental setups were obtained in the range of 3-34 nM and 11-113 nM, respectively. Standard addition experiments and the implementation of the internal ¹³C₄-DMA standard proved the accuracy of the quantification strategy even in complex matrices such as soil solution. The repeatability of the method, including sample preparation, expressed as short- and long term precision was below 4 and 5% RSD, respectively. Finally, application in the context of plant and soil research to samples from rhizosphere sampling via micro suction cups, from soil solutions and soil adsorption/extraction studies revealed a DMA concentration range from 0.1 to 235 μM.

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Section: Resultssupporting

confidence: 63%

Accurate LC‐ESI‐MS/MS quantification of 2′‐deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a ¹³C₄‐labeled internal standard

et al. 2014

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“…Zinc deficiency increases the secretion of MAs from wheat and barley roots into the rhizosphere (Cakmak et al . 1994; Walter et al .…”

Section: Discussionmentioning

confidence: 99%

Synthesis of nicotianamine and deoxymugineic acid is regulated by OsIRO2 in Zn excess rice plants

Ishimaru

Suzuki

Ogo

et al. 2008

Soil Science and Plant Nutrition

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Zinc (Zn) excess has significant toxicity to biological systems through metal-based cytotoxic reactions. Nicotianamine (NA) and deoxymugineic acid (DMA) are low-molecular-weight, high-affinity transition metal chelators. Studies have shown that NA may have a role in the tolerance of excess Zn. We show that a gene coding the iron (Fe)-regulated DNA-binding transcription factor (OsIRO2) and the downstream genes of OsIRO2, such as NA synthase, DMA synthase and the DMA-Fe 3+ transporter, were induced in rice roots by excess Zn. Consistent with the expression of these genes, the amounts of endogenous NA, endogenous DMA and DMA secretion increased in the excess Zn roots. Although the Fe concentration in the excess Zn roots was much higher than that in the control, rice ferritin gene, OsFer1, was downregulated in Zn excess roots. OsIRT1, which is upregulated by Fe deficiency, was not induced in Zn excess roots, suggesting that OsIRO2 may not be induced simply by the Fe deficiency caused by excess Zn. The data indicate that the induction of OsIRO2 by excess Zn is responsible for the production of NA and DMA, which may play a role in maintaining cellular Zn availability.

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“…It was reported that Zn deficiency increases the secretion of MAs from wheat and barley roots into the rhizosphere (Cakmak et al, 1994;Walter et al, 1994;Zhang et al, 1989). However, other studies have shown that significant secretion of MAs from barley or wheat only occurs in plants deficient in Fe, and not in those suffering Zn deficiency (Gries et al, 1995;Pedler et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis and secretion of mugineic acid family phytosiderophores in zinc‐deficient barley

et al. 2006

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SummaryMugineic acid family phytosiderophores (MAs) are metal chelators that are produced in graminaceous plants in response to iron (Fe) deficiency, but current evidence regarding secretion of MAs during zinc (Zn) deficiency is contradictory. Our studies using HPLC analysis showed that Zn deficiency induces the synthesis and secretion of MAs in barley plants. The levels of the HvNAS1, HvNAAT-A, HvNAAT-B, HvIDS2 and HvIDS3 transcripts, which encode the enzymes involved in the synthesis of MAs, were increased in Zn-deficient roots. Studies of the genes involved in the methionine cycle using microarray analysis showed that the transcripts of these genes were increased in both Zn-deficient and Fe-deficient barley roots, probably allowing the plant to meet its demand for methionine, a precursor in the synthesis of MAs. In addition, HvNAAT-B transcripts were detected in Zn-deficient shoots, but not in those that were deficient in Fe. Increased synthesis of MAs in Zndeficient barley was not due to a deficiency of Fe, because Zn-deficient barley accumulated more Fe than did the control plants, ferritin transcripts were increased in Zn-deficient plants, and Zn deficiency promoted Fe transport from root to shoot. Moreover, analysis using the positron-emitting tracer imaging system (PETIS) confirmed that more 62 Zn(II)-MAs than 62 Zn 2þ were absorbed by the roots of Zn-deficient barley plants. These data suggest that the increased biosynthesis and secretion of MAs arising from a shortage of Zn are not due to an induced Fe deficiency, and that secreted MAs are effective in absorbing Zn from the soil.

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Efect of zinc and iron deficiency on phytos1derophore release in wheat genotypes differing in zinc efficiency

Cited by 172 publications

References 36 publications

Accurate LC‐ESI‐MS/MS quantification of 2′‐deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a ¹³C₄‐labeled internal standard

Accurate LC‐ESI‐MS/MS quantification of 2′‐deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a ¹³C₄‐labeled internal standard

Synthesis of nicotianamine and deoxymugineic acid is regulated by OsIRO2 in Zn excess rice plants

Biosynthesis and secretion of mugineic acid family phytosiderophores in zinc‐deficient barley

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Efect of zinc and iron deficiency on phytos1derophore release in wheat genotypes differing in zinc efficiency

Cited by 172 publications

References 36 publications

Accurate LC‐ESI‐MS/MS quantification of 2′‐deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a 13C4‐labeled internal standard

Accurate LC‐ESI‐MS/MS quantification of 2′‐deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a 13C4‐labeled internal standard

Synthesis of nicotianamine and deoxymugineic acid is regulated by OsIRO2 in Zn excess rice plants

Biosynthesis and secretion of mugineic acid family phytosiderophores in zinc‐deficient barley

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Accurate LC‐ESI‐MS/MS quantification of 2′‐deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a ¹³C₄‐labeled internal standard

Accurate LC‐ESI‐MS/MS quantification of 2′‐deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a ¹³C₄‐labeled internal standard