Ef2: a new LY-3-linked light-chain marker expressed in normal mouse serum immunoglobulin.

Abstract

A new light-chain marker has been detected in normal mouse serum immunoglobulin light chains by gel isoelectric focusing. The marker (Ef2) involves the presence of two major and several minor bands in the normal light-chain IF profiles. Strains expressing the marker IF bands are designated Igk-Ef2a, whereas those lacking the bands are Igk-Ef2b. The majority of inbred strains are Igk-Ef2a. Strains found to be Igk-Ef2b are NZB/BlNJ, BDP/J, C58/J, I/LnJ, CE/J, and P/J. The strain distribution of the alleles differs from the distribution of alleles at the Ly-2 and Ly-3 loci, suggesting the new marker may represent a separate locus. Genetic studies have shown that Igk-Ef2 locus is closely linked to Igk-Ef1 and Hd loci on Chromosome 6, indicating that it is also closely linked to Ly-3. The relative importance of the bands controlled by the Igk-Ef2 locus suggests that the entire normal light-chain pool could be controlled by as few as 100 such loci.

LFA‐1 and Lyt‐2,3, Molecules Associated with T Lymphocyte‐Mediated Killing; and Mac‐1, an LFA‐1 Homologue Associated with Complement Receptor Function1

“…The simplest interpretation of this would be that Lyt-2,3 is a receptor for H-2K,D as shown in Figure 5 A, but there is no direct evidence for this. In view of the suggestions that Lyt-2,3 is a receptor, the close linkage of immunoglobulin kappa chain and Lyt-2,3 genes on chromosome 6 (Gibson & MacLean 1979) is extremely tantalizing. VL BIOCHEMICAL STRUCTURE OF LFA-1 AND ITS HOMOLOGY TO The above studies suggest that LFA-1 and Lyt-2,3 contribute to the avidity of CTL for targets, but a detailed understanding of their mechanism of action is dependent on biochemical characterization.…”

A major group of mouse kappa chains controlled by the chromosome 6 locus, IgK-Ef2.

“…In these studies, we believe we have identified one of the important V, groups involved in the Ef2 polymorphism previously described (5). Sequencing of the first three of the eight BALB/c myelomas found to possess light chains corresponding to the Ef2 marker bands (63 and 54) revealed an identical sequence over the first 30…”

“…This survey led to the identification of 8 BALB/c myelomas (out of a total of 277 examined) that have light chains indistinguishable from the marker light chain bands. In contrast, no light chains that cofocused precisely with the marker bands were identified among a total of 133 NZB myelomas (5).…”

“…Procedures for purification of normal immunoglobulin, light chain isolation, and IF of normal light chains have been described (3,5). For the analysis of myeloma ascites fluids, 1/~1 of fluid was subjected to complete reduction and alkylation with [*4C]iodoacetamide (50 mCi/ mmol; Amersham Corp., Arlington Heights, Ill.), as described.…”

“…We have concentrated our attention on the structural differences controlled by the lgK-Ef2 locus (5). This locus controls differences in normal light chain isoelectrie focusing (IF) patterns, and it has been shown to be closely linked to two chromosome 6 markers, Ly-3 and Hd (5).…”