EEC/COLIPA project on in vitro phototoxicity testing: First results obtained with a Balb/c 3T3 cell phototoxicity assay

Spielmann, Horst; Balls, Michael; Brand, Michael; Döring, B.; Holzhütter, Hermann−Georg; Kalweit, S.; Klecak, G.; Eplattenier, H.L.; Liebsch, Manfred; Lovell, W.W.; Maurer, Tobias; Moldenhauer, F.; Moore, Lindsey; Pape, Wolfgang; Pfanenbecker, U.; Potthast, Jörg; Silva, O. De; Steiling, W.; Willshaw, A.

doi:10.1016/0887-2333(94)90069-8

Cited by 110 publications

(54 citation statements)

References 5 publications

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“…Note that the ">" symbol assigned to the Lomefloxacin PIF value indicates that the IC 50 value for cells kept in the dark (-UVA) was not achieved and that the highest concentration of the test article tested was included in the numerator of the PIF formula. Spielmann et al (1994) also reported an IC 50 (ÀUVA) concentration value of 24.3 mg/L and a PIF value of 6 for Amiodarone indicating a true "phototoxic" potential. 20.2.1.5 Difficulties Associated with the 3T3 NRU Phototoxicity Test As indicated previously, there have been difficulties associated with this assay.…”

Section: The 3t3 Nru Phototoxicity Testmentioning

confidence: 99%

Phototoxicity and Photocarcinogenicity

Osterberg¹,

Sambuco

Forbes³

2013

Nonclinical Safety Assessment

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Section: The 3t3 Nru Phototoxicity Testmentioning

confidence: 99%

Phototoxicity and Photocarcinogenicity

Osterberg¹,

Sambuco

Forbes³

2013

Nonclinical Safety Assessment

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“…In the prevalidation study conducted with 20 test chemicals, the 3T3 NRU PT in-vitro phototoxicity test was unexpectedly the only such test in which all 20 chemicals were correctly identifi ed as phototoxic or non-phototoxic [16,17]. Quite independently, a laboratory in Japan subsequently obtained the same correct results in the 3T3 NRU PT, when testing the same set of 20 test chemicals.…”

Section: In-vitro Phototoxicity Testmentioning

confidence: 99%

Ethical Environment and Scientific Rationale Towards In‐Vitro Alternatives to Animal Testing: Where Are We Going?

Spielmann

2006

Drug Testing in Vitro

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“…78 (EURL ECVAM DB-ALM; Spielmann et al, 1994aSpielmann et al, , 1994b. Balb/3T3 clone A31 cells in the logarithmic growth phase were seeded in a 96-well assay plate at 10 4 cells/well and cultured for 24 hr (37°C, 5% CO 2 ).…”

Section: Conventional Methodsmentioning

confidence: 99%

Development of a modified 3T3 Neutral Red Uptake Phototoxicity Test protocol for evaluation of poorly water-soluble substances

et al. 2017

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-The 3T3 neutral red uptake phototoxicity test (OECD TG432) is an alternative phototoxicity test method that is relatively easy and rapid to implement, with results obtainable in a short time, and is reported to have high reproducibility compared with in vivo assay methods. However, this method has been shown to be unsuitable for testing poorly water-soluble substances, which tend to separate out when mixed with the assay buffer solution. This causes difficulties in determining the dose dependency of substances and subsequent determination of the photoirritation factor because the ratio of cell viability, expressed as the half-maximal inhibitory concentration (IC 50 ) in the presence or absence of light, is not calculable. In this study, we investigated the optimum conditions for the evaluation of poorly water-soluble substances. In the conventional method, the final solvent concentration was 1% and the pre-incubation time was 60 min, but in the modified method, 10% and 5 min were used, respectively. Next, the results from the conventional method were compared with those of our modified method, which was found to be viable and comparable with the conventional method. Moreover, the false positive results frequently obtained with poorly water-soluble substances in the conventional method were not evident with the modified method, thus confirming its usefulness for the evaluation of such substances. We therefore propose that the modified method can be used for the in vitro testing of poorly water-soluble substances in phototoxicity evaluations.

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EEC/COLIPA project on in vitro phototoxicity testing: First results obtained with a Balb/c 3T3 cell phototoxicity assay

Cited by 110 publications

References 5 publications

Phototoxicity and Photocarcinogenicity

Phototoxicity and Photocarcinogenicity

Ethical Environment and Scientific Rationale Towards In‐Vitro Alternatives to Animal Testing: Where Are We Going?

Development of a modified 3T3 Neutral Red Uptake Phototoxicity Test protocol for evaluation of poorly water-soluble substances

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