EDTA induced changes in platelet structure and function: influence on particle uptake

Abstract

Ethylenediaminetetraacetic acid (EDTA) causes structural, biochemical and functional damage to blood platelets. The alterations induced are considered irreversible. However, the degree of irreversibility, and whether all functions are similarly compromised by EDTA have not been fully evaluated. The present study has examined platelets treated with EDTA to produce the structural changes in channels of the open canalicular system (OCS) associated with irreversible dissociation of the fibrinogen receptor, GPIIb-IIIa (alpha(IIb)beta(3)), for their ability to interact with particulates in suspension. Despite severe narrowing and near occlusion of peripherally oriented OCS channels by EDTA, treated cells were able to bind, translocate and take up fibrinogen-coated gold particles (Fgn/Au), colloidal gold and latex spheres. Thus exposure to EDTA may compromise some aspects of platelet function, but not others which may be important for participation in hemostatic events.

Contrast-enhanced imaging of SPIO-labeled platelets using magnetomotive ultrasound

“…Platelets have been shown to readily take up nanoparticles via immune processes (White et al 1999) and when labeled with imaging contrast agents, provide a platform for functional imaging. Since the 1970s, platelets have been used as radio labeled contrast agents in gamma ray imaging for the study of blood related diseases (Fuster et al 1979).…”