EDTA dependent pseudothrombocytopenia caused by antibodies against the cytoadhesive receptor of platelet gpIIB-IIIA.

Casonato, Alessandra; Bertomoro, Antonella; Pontara, Elena; Dannhauser, D.; Ar, Lazzaro; Girolami, Antonio

doi:10.1136/jcp.47.7.625

Cited by 82 publications

(86 citation statements)

References 26 publications

(4 reference statements)

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“…1,10,22,24,36 In humans, EDTA-dependent platelet clumping is presumptively affected by agglutinating antibodies that recognize cytoadhesive receptors on platelet GPIIb-IIIa and/or by calcium chelation-triggered conformational changes in the cellular membrane. 2,17,18 Notably, in the present study, there was a significant variability of the frequency of platelet clumping among dogs, which accounted for 51% of the total variation. This finding is in accordance with the results of a previous study, 9 in which the distribution and the size of the platelet clumps was not always homogeneous in the blood smears, indicating that the evaluation of a single smear may not be an accurate reflection of the overall degree of platelet clumping in a particular sample.…”

Section: Discussionmentioning

confidence: 85%

Effect of Anticoagulant and Storage Conditions on Platelet Size and Clumping in Healthy Dogs

et al. 2008

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Abstract. The potential impact of preanalytical factors, such as type of anticoagulant, storage temperature, and time, on the formation of macroplatelets and platelet aggregates (platelet clumping) in dogs is largely elusive. The objective of the current study was to assess the effect of anticoagulant, temperature, and blood storage time in the light microscopy-generated macroplatelet percentages and the frequency of visually inspected platelet aggregates in clinically healthy dogs. Giemsa-stained blood smears from 70 healthy dogs were reviewed after exposure to different anticoagulants (ethylenediamine tetra-acetic acid [EDTA] vs. citrate), temperatures (25uC vs. 4uC), and storage times (up to 24 hr postsampling). The mean percentage of macroplatelets (platelets with diameter or length $5 mm) was higher (P 5 0.0006) when EDTA was used as the anticoagulant. For either anticoagulant, the mean percentage of macroplatelets was higher (P , 0.0001) at 25uC than at 4uC. Platelet clumping was 1.9 times (P , 0.0001) more likely to occur when citrate-rather than EDTA-anticoagulated blood was examined; regardless of the anticoagulant used, clumping occurred 3 times (P , 0.0001) more often when samples were preserved at 4uC than when they were preserved at 25uC. Storage time did not significantly influence the macroplatelet percentages or the frequency of platelet clumping. The results of this study indicate that macroplatelet percentages in the canine blood should be interpreted in relation to anticoagulant-and temperature-specific reference intervals and that future studies are warranted in order to investigate the clinical relevance of this calculation. In addition, the significant association of citrate with the formation of platelet aggregates may preclude its use for platelet enumeration in the dog.

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Section: Discussionmentioning

confidence: 85%

Effect of Anticoagulant and Storage Conditions on Platelet Size and Clumping in Healthy Dogs

et al. 2008

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“…The platelet agglutination in the presence of EDTA is because of a reaction with circulating EDTA-sensitive antibodies. Platelet clumping is then caused by an immunoglobulin recognising an epitope exposed on platelets in vitro [10]. EDTA-induced degranulation has been very rarely reported [11].…”

Section: Discussionmentioning

confidence: 99%

“…Her initial CBC carried out in EDTA sample revealed Hb 10.8 mg/dl (10.1-12.3), RBC count 3.98 million/mm 3 (4.1-5.0), normal RBC indices, WBC count 24,100/mm 3 (6,000-18,000), with DLC comprising of N 12%, Eo 40% (AEC-9,640/mm 3 ), L 47%, M 0.1%. She had a platelet count of 2.02 lakhs/mm 3 and MPV was 12.1 fl (7)(8)(9)(10)(11). The microscopic examination of the PB smear confirmed eosinophilia but also revealed the presence of hardly visible platelets ( Fig.…”

Section: Casementioning

confidence: 98%

Pseudo-Grey Platelet Syndrome: A Rare Artifact

Agarwal

2011

Indian J Hematol Blood Transfus

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Two cases that were identified during routine blood examination, presented an artifact the pseudo-grey platelet syndrome. The platelets from the blood collected with ethylene diamine tetra-acetic acid (EDTA) stained poorly on the blood smear and appeared agranular under the microscope. This artifactual anomaly did not occur when samples were collected in vacutainers containing citrate or collected by finger prick. Reports of pseudo-grey platelet syndrome are few, possibly because of the poorly explained and difficult to diagnose phenomenon.

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“…Ancak EDTA, nadiren de olsa trombositlerin kümeleşmesiyle karakterize bir psödot-rombositopeniye neden olabilir. Trombositlerin bu kü-meleşmesinden trombosit yüzey antijenlerine karşı oluş-muş IgM, IgG ve IgA yapısındaki otoantikorlar sorumludur [1][2][3]. Düşük sıcaklık ve EDTA'nın kalsiyum iyonlarına bağlanırken oluşturduğu kombine etki, normalde saklı olan ve trombosit yüzeyinde bulunan glikoprotein IIb/IIIa molekülünü etkileyerek glikoprotein IIb epitopunu açığa çıkarır.…”

Section: Introductionunclassified

EDTA-dependent pseudothrombocytopenia in a patient with the prediagnosis of idiopathic thrombocytopenic purpura

Özçeli̇k¹,

Öztosun²,

Gülsün³

et al. 2012

Turk J Bioch

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EDTA dependent pseudothrombocytopenia caused by antibodies against the cytoadhesive receptor of platelet gpIIB-IIIA.

Cited by 82 publications

References 26 publications

Effect of Anticoagulant and Storage Conditions on Platelet Size and Clumping in Healthy Dogs

Effect of Anticoagulant and Storage Conditions on Platelet Size and Clumping in Healthy Dogs

Pseudo-Grey Platelet Syndrome: A Rare Artifact

EDTA-dependent pseudothrombocytopenia in a patient with the prediagnosis of idiopathic thrombocytopenic purpura

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